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− | <div class="title"> RESHAPE: Tuning morphology and secretion in Aspergillus niger for improved industrial applications</div> | + | <div class="title"> RESHAPE: Tuning morphology and secretion in <i>Aspergillus niger</i> for improved industrial applications</div> |
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| <p style="font-size:10px"> <sup>a</sup>DTU Bioengineering, <sup>b</sup>DTU Health Tech, Technical University of Denmark </p> | | <p style="font-size:10px"> <sup>a</sup>DTU Bioengineering, <sup>b</sup>DTU Health Tech, Technical University of Denmark </p> |
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− | <b>Abstract:</b> Every week, almost 6 billion people use products made with the aid of fungal cell factories. Many of these fungi stem from the Aspergillus genus. We aim to improve the production efficiency of Aspergillus niger by creating a synthetic biology toolbox that focuses on mycelial morphology and secretion. Morphology has a significant effect on productivity of certain compounds, while proper secretion is important for later recovery and purification of a compound. We have approached our goal in three ways: | + | <b>Abstract:</b> Every week, almost 6 billion people use products made with the aid of fungal cell factories. Many of these fungi stem from the Aspergillus genus. We aim to improve the production efficiency of <i>Aspergillus niger</i> by creating a synthetic biology toolbox that focuses on mycelial morphology and secretion. Morphology has a significant effect on productivity of certain compounds, while proper secretion is important for later recovery and purification of a compound. We have approached our goal in three ways: |
| <ol type="1"> | | <ol type="1"> |
| <li>Characterising morphological changes of A. niger by engineering seven morphology related genes. </li> | | <li>Characterising morphological changes of A. niger by engineering seven morphology related genes. </li> |
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| Shifting production of industrially important compounds such as enzymes, to be less reliant on petrochemicals, would alleviate some of the stress which is put on the current supply chains. One way of making this shift is by using filamentous fungi as cell factories. For many compounds which could be produced by cell factories, however, the cost and nuisance of working with filamentous fungi is prohibitive to their widespread use. | | Shifting production of industrially important compounds such as enzymes, to be less reliant on petrochemicals, would alleviate some of the stress which is put on the current supply chains. One way of making this shift is by using filamentous fungi as cell factories. For many compounds which could be produced by cell factories, however, the cost and nuisance of working with filamentous fungi is prohibitive to their widespread use. |
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− | We hoped to contribute to the development of more sustainable production practices by improving the filamentous fungus Aspergillus niger as a cell factory. | + | We hoped to contribute to the development of more sustainable production practices by improving the filamentous fungus <i>Aspergillus niger</i> as a cell factory. |
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− | Genetic engineering of the Aspergillus niger genome can be achieved using various methods, including CRISPR-Cas9 recently adapted from Streptococcus pyogenes (Nodvig et al., 2018). | + | Genetic engineering of the <i>Aspergillus niger</i> genome can be achieved using various methods, including CRISPR-Cas9 recently adapted from Streptococcus pyogenes (Nodvig et al., 2018). |
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| Here, we present our extensive collection of CRISPR-Cas9 parts for engineering the morphology of A. niger. The presented collection comprises 40 parts for knockout of a total of 7 genes with morphological relation. These parts can be combined to make a wide range of knockouts. Transform one plasmid into protoplasts for a single knockout - confirm the knockout and make protoplasts of the mutant - transform a second plasmid into the mutant protoplasts - confirm and make protoplasts - transform… You get the drill. | | Here, we present our extensive collection of CRISPR-Cas9 parts for engineering the morphology of A. niger. The presented collection comprises 40 parts for knockout of a total of 7 genes with morphological relation. These parts can be combined to make a wide range of knockouts. Transform one plasmid into protoplasts for a single knockout - confirm the knockout and make protoplasts of the mutant - transform a second plasmid into the mutant protoplasts - confirm and make protoplasts - transform… You get the drill. |
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| <b>Morphological toolbox for A. niger</b><br> | | <b>Morphological toolbox for A. niger</b><br> |
− | We have made a collection of CRISPR-Cas9 parts that can be used to engineer morphology in Aspergillus niger. This part collection consists of in total 40 parts including seven CRISPR-Cas9 vectors that can be used to knockout 7 morphology related genes in A. niger. The parts can be combined in endless ways to help engineer morphology in the future. | + | We have made a collection of CRISPR-Cas9 parts that can be used to engineer morphology in <i>Aspergillus niger</i>. This part collection consists of in total 40 parts including seven CRISPR-Cas9 vectors that can be used to knockout 7 morphology related genes in A. niger. The parts can be combined in endless ways to help engineer morphology in the future. |
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| <b>Analysis of fungal microscopic images</b><br> | | <b>Analysis of fungal microscopic images</b><br> |