Difference between revisions of "Team:Paris Bettencourt/Poster"

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                                 <p>As this situation was unprecedented, we were wondering if the lockdown could have an
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                                 <p id="padding-left">As this situation was unprecedented, we were wondering if the lockdown could have an
 
                                     impact on human health and more specifically on the composition of our skin
 
                                     impact on human health and more specifically on the composition of our skin
 
                                     microbiome.
 
                                     microbiome.
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                                 <p>We met some dermatologists as Stéphanie Leclerc-Mercier. She pointed out the fact
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                                 <p id="padding-right">We met some dermatologists as Stéphanie Leclerc-Mercier. She pointed out the fact
 
                                     that the new sanitary measures and hygiene habits imposed by the pandemic were a
 
                                     that the new sanitary measures and hygiene habits imposed by the pandemic were a
 
                                     real issue for people with eczema symptoms since they cannot wash their hands as
 
                                     real issue for people with eczema symptoms since they cannot wash their hands as
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                                 <p>Our team personal values led us to create a project who enters in the framework of
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                                 <p id="padding-right">Our team personal values led us to create a project who enters in the framework of
 
                                     Open and Citizen Science. Especially during the lockdown which forced us to be
 
                                     Open and Citizen Science. Especially during the lockdown which forced us to be
 
                                     physically isolated of each other, we wanted to find a way to connect people through
 
                                     physically isolated of each other, we wanted to find a way to connect people through

Revision as of 19:18, 10 November 2020

SynDerma - The Skin Microbiome in the Spotlight:
From Sampling to Engineering

Presented by Team Paris Bettencourt 2020


Amandine Maire1, Anu Susan Kurian1, Chetan Kumar Velumurugan1, Nicolas Levrier1, Nikola Zarevski1, Valerie March1, Xavier Olessa-Daragon1, Ariel Lindner2, Jake Wintermute2, Radoslaw Ejsmont2, Alexis Casas2, Darshak Bhatt2


1Student Team Member, 2Team Mentor


Abstract

Objectives

1. Sample and Sequence the Human Skin Microbiome

Our first objective, pursued as part of the Quaranskin project, is to study the impact of environmental and behavioral factors on the skin microbiome and to provide a new database to study its composition in the context of reduced social and environmental interactions.


2. Design tools to engineer the bacterium S. epidermidis

Our second objective is to make S. epidermidis an efficient synthetic biology chassis that can be used to monitor the population dynamics of the skin microbiome. This in order to maintain its equilibrium and to avoid pathologies induced by dysbiosis.

Inspiration

In the context of COVID-19 pandemic, we were, as most of the other iGEM teams confined at home.



As this situation was unprecedented, we were wondering if the lockdown could have an impact on human health and more specifically on the composition of our skin microbiome.


We met some dermatologists as Stéphanie Leclerc-Mercier. She pointed out the fact that the new sanitary measures and hygiene habits imposed by the pandemic were a real issue for people with eczema symptoms since they cannot wash their hands as often as healthy people. This discussion drew our interest for dysbiosis induced pathologies and ways to treat them using synthetic biology.




Our team personal values led us to create a project who enters in the framework of Open and Citizen Science. Especially during the lockdown which forced us to be physically isolated of each other, we wanted to find a way to connect people through science.

Challenges

1. Study the Skin Microbiome

Human skin microbiome sampling is a challenge in itself since it implies reaching out to a large cohort and collecting human being derived samples. In the context of a pandemic other constraints are added because of social distancing measures.


2. Engineer the Skin Microbiome

  • Staphylococcus epidermidis is not used in synthetic biology and no tools are currently available.
  • In addition to that two main challenges remain to use S. epidermidis in synthetic biology
Responses

1. Quaranskin

Quaranskin is a study collecting skin microbiome samples during the COVID-19 pandemic and observing the impact of environmental factors on the skin microbiome.

  • We took ethical considerations very seriously by submitting our project to the french national ethics committee in order to obtain their approval before starting the study
  • In order to include citizen participation, we are setting up a mailing kit system containing all the material necessary for skin microbiome sampling.
  • We are building an Open Database of skin microbiome in this unpreceded context of pandemic.

2. EpiFlex, EpiGlow, EpiGrow

These three projects aims to develop tools to make S. epidermidis a good chassis for Synthetic biology.

  • EpiFlex is a MoClo tool kit for S. epidermidis
  • EpiGlow is the proof of concept of EpiFlex that aims to optimize transformation protocol S. epidermidis
  • EpiGrow is the optimization of S. epidermidis growth
Skin Microbiome
Here you can explain the engineering process behind your project.
Engineering
Here you can explain the engineering process behind your project.
Engineering
Here you can explain the engineering process behind your project.
Engineer
Objectives 1. Investigate the impact of behavioural factors on the skin microbiome and provide novel data base to explore its composition within the context of a pandemic.

Objectives 2. Develop tools to engineer S. epidermidis.
Control populations dynamics of the skin microbiome in order to maintain its equilibrium and avoid dysbiosis induced pathologies.
Section 1
Use this section to explain whatever you would like! Suggestions: Safety, Human Practices, Measurement, etc.
Section 2
Use this section to explain whatever you would like! Suggestions: Safety, Human Practices, Measurement, etc.
Section 3
Use this section to explain whatever you would like! Suggestions: Safety, Human Practices, Measurement, etc.
Results
What did your team achieve? What do you plan to work on moving forward?
References and Acknowledgements
If not already cited in other sections of your poster, what literature sources did you reference on this poster? Who helped or advised you?
iGEM
Here you can explain the engineering process behind your project.
Acknowledgements
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