|
|
Line 102: |
Line 102: |
| correlate the diversity and composition of those microbiome-data, to behaviours noted in | | correlate the diversity and composition of those microbiome-data, to behaviours noted in |
| the surveys, to uncover any trends. In parallel, we chose the skin commensal microbe | | the surveys, to uncover any trends. In parallel, we chose the skin commensal microbe |
− | Staphylococcus epidermidis to be a chassis for our future vision of microbial | + | <i>Staphylococcus epidermidis</i> to be a chassis for our future vision of microbial |
| therapeutics enabled by synthetic biology. In projects EpiFlex, EpiGlow, and EpiGrow, we | | therapeutics enabled by synthetic biology. In projects EpiFlex, EpiGlow, and EpiGrow, we |
| built a MoClo kit, expressed fluorescent proteins as a proof of concept, and optimised | | built a MoClo kit, expressed fluorescent proteins as a proof of concept, and optimised |
Line 138: |
Line 138: |
| <p><b class="heading">2. Design tools to engineer the bacterium <i>S. epidermidis</i></b> | | <p><b class="heading">2. Design tools to engineer the bacterium <i>S. epidermidis</i></b> |
| </p> | | </p> |
− | <p>Our second objective is to make S. epidermidis an efficient synthetic biology chassis | + | <p>Our second objective is to make <i>S. epidermidis</i> an efficient synthetic biology chassis |
| that can be used to monitor the population dynamics of the skin microbiome. This in | | that can be used to monitor the population dynamics of the skin microbiome. This in |
| order to maintain its equilibrium and to avoid pathologies induced by dysbiosis. | | order to maintain its equilibrium and to avoid pathologies induced by dysbiosis. |
Line 290: |
Line 290: |
| <br /> | | <br /> |
| <p><b class="heading">2. EpiFlex, EpiGlow, EpiGrow</b></p> | | <p><b class="heading">2. EpiFlex, EpiGlow, EpiGrow</b></p> |
− | <p>These three projects aims to develop tools to make S. epidermidis a good chassis for | + | <p>These three projects aims to develop tools to make <i>S. epidermidis</i> a good chassis for |
| Synthetic biology. | | Synthetic biology. |
| </p> | | </p> |
Line 331: |
Line 331: |
| the skin by pathological microorganisms, and help to train the immune system to make it | | the skin by pathological microorganisms, and help to train the immune system to make it |
| more prepared to face pathogens. It also directly involved in various pathologies, like | | more prepared to face pathogens. It also directly involved in various pathologies, like |
− | atopic dermatitis, where its overall diversity is reduced and Staphylococcus aureus is | + | atopic dermatitis, where its overall diversity is reduced and <i>Staphylococcus aureus</i> is |
| over represented. | | over represented. |
| </p> | | </p> |
Line 557: |
Line 557: |
| <!--Write the text explaining this section --> | | <!--Write the text explaining this section --> |
| <div class="text"> | | <div class="text"> |
− | <p>The bacterium Staphylococcus epidermidis has only few tools available for efficient | + | <p>The bacterium <i>Staphylococcus epidermidis</i> has only few tools available for efficient |
| expression of recombinant DNA and genetic engineering. This generated the idea to | | expression of recombinant DNA and genetic engineering. This generated the idea to |
| develop a MoClo toolkit for S.epidermidis, the EpiFlex toolkit. | | develop a MoClo toolkit for S.epidermidis, the EpiFlex toolkit. |
Line 673: |
Line 673: |
| </li> | | </li> |
| <li>Chen, Y. Erin, et al. "Decoding commensal-host communication through genetic | | <li>Chen, Y. Erin, et al. "Decoding commensal-host communication through genetic |
− | engineering of Staphylococcus epidermidis."bioRxiv(2019): 664656. | + | engineering of <i>Staphylococcus epidermidis</i>."bioRxiv(2019): 664656. |
| </li> | | </li> |
| </ol> | | </ol> |
Line 700: |
Line 700: |
| src="https://static.igem.org/mediawiki/2020/3/3e/T--Paris_Bettencourt--Poster_EpiGrowLogo.png"> | | src="https://static.igem.org/mediawiki/2020/3/3e/T--Paris_Bettencourt--Poster_EpiGrowLogo.png"> |
| | | |
− | <p>We used the S. epidermidis strain ATCC12228 | + | <p>We used the <i>S. epidermidis</i> strain ATCC12228 |
| </p> | | </p> |
| | | |
Line 730: |
Line 730: |
| <p><b class="heading">Results</b></p> | | <p><b class="heading">Results</b></p> |
| <ol> | | <ol> |
− | <li>The growth of S. epidermidis is stunted at lower temperatures, and it grows best at | + | <li>The growth of <i>S. epidermidis</i> is stunted at lower temperatures, and it grows best at |
| 37°C which is the normal human body temperature. | | 37°C which is the normal human body temperature. |
| </li> | | </li> |
Line 739: |
Line 739: |
| </li> | | </li> |
| </ol> | | </ol> |
| + | <br/> |
| + | |
| <img | | <img |
| src="https://static.igem.org/mediawiki/2020/b/b6/T--Paris_Bettencourt--Poster_EpiGrowGrowthRates.png"> | | src="https://static.igem.org/mediawiki/2020/b/b6/T--Paris_Bettencourt--Poster_EpiGrowGrowthRates.png"> |
− | | + | <br/> |
| + | |
| <p>References</p> | | <p>References</p> |
| <ol> | | <ol> |
Line 825: |
Line 828: |
| <p><b class="heading">EpiGrow</b></p> | | <p><b class="heading">EpiGrow</b></p> |
| <ul> | | <ul> |
− | <li>We would like to investigate the difference of growth kinetic between S. epidermidis | + | <li>We would like to investigate the difference of growth kinetic between <i>S. epidermidis</i> |
| grown in 2D and in 3D media. | | grown in 2D and in 3D media. |
| </li> | | </li> |