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− | <a class=" | + | <a class="mainitem" href="https://2020.igem.org/Team:Virginia">HOME</a> |
<div class="dropdown-content"> | <div class="dropdown-content"> | ||
<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia">Main</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia">Main</a> | ||
− | <a class="hvr-sweep-to-right" href="#abstract">Abstract | + | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia#abstract">Abstract</a> |
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<a class="mainitem" href="#project">PROJECT</a> | <a class="mainitem" href="#project">PROJECT</a> | ||
<div class="dropdown-content"> | <div class="dropdown-content"> | ||
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<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Description">Description</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Description">Description</a> | ||
<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Design">Design</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Design">Design</a> | ||
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<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Results">Results</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Results">Results</a> | ||
<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Model">Modeling</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Model">Modeling</a> | ||
+ | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Engineering">Engineering</a> | ||
<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Implementation">Implementation</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Implementation">Implementation</a> | ||
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<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Notebook">Notebook</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Notebook">Notebook</a> | ||
<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Safety">Safety</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Safety">Safety</a> | ||
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<a class="mainitem" href="#parts">PARTS</a> | <a class="mainitem" href="#parts">PARTS</a> | ||
<div class="dropdown-content"> | <div class="dropdown-content"> | ||
− | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/New_parts"> | + | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/New_parts"> Parts</a> |
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</div> | </div> | ||
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<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Members">Members</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Members">Members</a> | ||
<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Attributions">Attributions</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Attributions">Attributions</a> | ||
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<a class="mainitem" href="#about">RESOURCES</a> | <a class="mainitem" href="#about">RESOURCES</a> | ||
<div class="dropdown-content"> | <div class="dropdown-content"> | ||
− | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Papers"> | + | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Papers">Sources</a> |
<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/nucleic_acids">Nucleic Acids</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/nucleic_acids">Nucleic Acids</a> | ||
<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Protocols">Protocols</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Protocols">Protocols</a> | ||
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<div class="section"> | <div class="section"> | ||
<div class="paragraph"> | <div class="paragraph"> | ||
− | <li><b>Julia Ball</b>: As our team captain, member of the Human Practices and Wetlab Committees, Julia helped organize our team, communicated with other iGEM teams, advisors, and experts through almost 100 emails, helped fill out safety forms, organized the recruitment for next year’s team, and was the primary point of contact with the iGEM organization overall. Also, Julia wrote the code of ethical conduct, worked on the blog for the iGEM engineering school website, and helped develop the | + | <li><b>Julia Ball</b>: As our team captain, member of the Human Practices and Wetlab Committees, Julia helped organize our team, communicated with other iGEM teams, advisors, and experts through almost 100 emails, helped fill out safety forms, organized the recruitment for next year’s team, and was the primary point of contact with the iGEM organization overall. Also, Julia wrote the code of ethical conduct, worked on the blog for the iGEM engineering school website, and helped develop the Resource Hub and the Wiki. As part of the DNA subteam of the Wetlab committee, Julia helped design the DNA scaffold procedures and worked in the lab. </li> |
</div> | </div> | ||
<div class="paragraph"> | <div class="paragraph"> | ||
− | <li><b>Collin Marino</b>: As head of the Wetlab committee and member of the Wiki and Entrepreneurship committees, Collin was the member that originally came up with the project idea. As Wetlab lead, he spearheaded the initial planning of experimental procedures and oversaw the procedure development and flow chart design. In addition, Collin wrote and successfully filed the provisional patent, allowing the team to begin sharing our idea with experts and getting their feedback. Also, Collin did the coding for the Wiki and created a template for the pages, which allowed other team members to help | + | <li><b>Collin Marino</b>: As head of the Wetlab committee and member of the Wiki and Entrepreneurship committees, Collin was the member that originally came up with the project idea. As Wetlab lead, he spearheaded the initial planning of experimental procedures and oversaw the procedure development and flow chart design. In addition, Collin wrote and successfully filed the provisional patent, allowing the team to begin sharing our idea with experts and getting their feedback. Also, Collin did the coding for the Wiki and created a template for the pages, which allowed other team members to help add content to the wiki.</li> |
</div> | </div> | ||
<div class="paragraph"> | <div class="paragraph"> | ||
− | <li><b>Colin Haws</b>: As head of the Modeling committee and member of the Wetlab committee, Colin focused on leading the modeling efforts to describe the efficiency of the | + | <li><b>Colin Haws</b>: As head of the Modeling committee and member of the Wetlab committee, Colin focused on leading the modeling efforts to describe the efficiency of the Manifold system. More specifically he worked on the promotor calculator and the reaction kinetics models. In Wetlab, Colin helped in the collection and assembly of BMC parts, both in the lab and virtually, and worked on the BMC procedures in the lab with Veronica.</li> |
</div> | </div> | ||
<div class="paragraph"> | <div class="paragraph"> | ||
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</div> | </div> | ||
<div class="paragraph"> | <div class="paragraph"> | ||
− | <li><b> | + | <li><b>Aparna Kola</b>: As co-head of the Entrepreneurship Committee and a member of the Modeling and Human Practices Committee, Aparna worked to conduct industry research, and network with the stakeholders in the local community while organizing discussions regarding IP protection and patentability. In addition, she worked on the promoter calculator, flux balance analysis, and pore diffusion models. As part of HP, she helped to author the Code of Ethical conduct, organize the virtual Resource Hub, and worked on the blog for the iGEM engineering school website.</li> |
</div> | </div> | ||
<div class="paragraph"> | <div class="paragraph"> | ||
− | <li><b> | + | <li><b>Sophia Link</b>: As a member of the Wetlab and Wiki committees, Sophia worked on the design of the DNA scaffold and helped with the writeup and organization of Reverse transcriptase assembly Wetlab procedures. In addition, Sophia created most (if not all) of Manifold's graphics, including the one on our home page and the design of the flowcharts that can be seen on the experiments page. </li> |
</div> | </div> | ||
<div class="paragraph"> | <div class="paragraph"> | ||
− | <li><b> | + | <li><b>Veronica Gutierrez</b>: As a member of the Wetlab, Wiki, and Entrepreneurship committees, Veronica helped research and write procedures regarding the construction of the BMC, aided in the overall structure and organization of the wiki and helped collect details on current or pending patents. In the lab, Veronica worked with Colin Haws to go through the different procedures pertaining to BMC formation and analyze the results. </li> |
</div> | </div> | ||
+ | |||
<div class="paragraph"> | <div class="paragraph"> | ||
<li><b>Apekchha Pradhan</b>: As a member of the Wetlab, Entrepreneurship, and Wiki committees, Apekchha was in charge of forming the sequences and parts for the 4Cl and STS enzymes in the resveratrol pathway. She worked with Peitro and Jacob in the lab, following the protocols for their enzyme sub-team. She also took part in helping build the Wiki and got to be more familiar with web design and HTML/CSS. </li> | <li><b>Apekchha Pradhan</b>: As a member of the Wetlab, Entrepreneurship, and Wiki committees, Apekchha was in charge of forming the sequences and parts for the 4Cl and STS enzymes in the resveratrol pathway. She worked with Peitro and Jacob in the lab, following the protocols for their enzyme sub-team. She also took part in helping build the Wiki and got to be more familiar with web design and HTML/CSS. </li> | ||
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<li><b>Dr. George McArthur</b>: Further into the summer, we reached out to Dr. McArthur, the founder of the Virginia iGEM Team, Director of Genetic Design-Build at Arzeda, and new Head of Product at Ansa Biotechnologies, Inc. We did a short presentation for him on the Wetlab component of our project, and he gave us advice on next steps for the project: get funding, find other ways to market the device (ie. expand its applications) and figure out how to get data without having normal access to our lab (outsource to Teselagen/Honeybee/Culture Biosciences). </li> | <li><b>Dr. George McArthur</b>: Further into the summer, we reached out to Dr. McArthur, the founder of the Virginia iGEM Team, Director of Genetic Design-Build at Arzeda, and new Head of Product at Ansa Biotechnologies, Inc. We did a short presentation for him on the Wetlab component of our project, and he gave us advice on next steps for the project: get funding, find other ways to market the device (ie. expand its applications) and figure out how to get data without having normal access to our lab (outsource to Teselagen/Honeybee/Culture Biosciences). </li> | ||
</div> | </div> | ||
+ | <div class="paragraph"> | ||
+ | <li><b>Dr. Stacey Joe Criswell</b>: Dr. Criswell, who works at the University of Virginia’s Advanced Microscopy Facility, was happy to help us with our TEM images of our BMCs. We are especially thankful for her patience and flexibility.</li></div> | ||
+ | <div class="paragraph"> | ||
+ | <li><b>Dr. Deborah Roach</b>: We would like to thank Dr. Roach, the Chair of Biology at the Univeristy of Virginia, who provided the laboratory space and the funds to help run the lab.</li></div> | ||
+ | <div class="paragraph"> | ||
+ | <li><b>Dr. Anthony Spano</b>: Dr. Spano, a Research Assistant Professor of Biology at the Univeristy of Virginia, graciously provided us with the BL21(DE3) <i>E. coli</i> cells that were used throughout our experiments. </li></div> | ||
+ | <div class="paragraph"> | ||
+ | <li><b>Dr. Dorothy Schafer</b>: We would also like to extend a huge thank you to Dr. Schafer for donating various restriction enzymes, chemicals, lab supplies, the PCR machine, and much more. </li></div> | ||
</div> | </div> | ||
<div class="sectionTitle" id="Section 4">Human Practices Advising and Support</div> | <div class="sectionTitle" id="Section 4">Human Practices Advising and Support</div> | ||
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<li><b>Professor Mimi Riley</b>: Now armed with the relevant questions and our tentative answers, we reached out to Professor Riley, a professor of law, PHS, and public policy at UVA, and the chair of UVA's Embryonic Stem Cell Research Oversight Committee, as well as a legal advisor to the Health Sciences Institutional Review Board. In our first meetings, we asked general questions regarding synthetic biology society, and our conversations gradually shifted to discussing the current social movements occurring in the country, and if we have a role as a research team to take a stance on such issues. We spoke intensely about our capability to positively and directly impact our community as a team of researchers, and we learned the true difficulty of going beyond merely making a statement and moving on. Through this, Professor Riley encouraged us to think internally first and to detail our own responsibilities and actions before blindly charging into the public eye. She further endorsed the idea of a COEC, as a means to debate and understand our values as a team and how they affect the work we put out into society. Professor Riley later reviewed our COEC gave us direct advice on individual statements and sections, and how to finalize this document for others to adapt and improve.</li> | <li><b>Professor Mimi Riley</b>: Now armed with the relevant questions and our tentative answers, we reached out to Professor Riley, a professor of law, PHS, and public policy at UVA, and the chair of UVA's Embryonic Stem Cell Research Oversight Committee, as well as a legal advisor to the Health Sciences Institutional Review Board. In our first meetings, we asked general questions regarding synthetic biology society, and our conversations gradually shifted to discussing the current social movements occurring in the country, and if we have a role as a research team to take a stance on such issues. We spoke intensely about our capability to positively and directly impact our community as a team of researchers, and we learned the true difficulty of going beyond merely making a statement and moving on. Through this, Professor Riley encouraged us to think internally first and to detail our own responsibilities and actions before blindly charging into the public eye. She further endorsed the idea of a COEC, as a means to debate and understand our values as a team and how they affect the work we put out into society. Professor Riley later reviewed our COEC gave us direct advice on individual statements and sections, and how to finalize this document for others to adapt and improve.</li> | ||
</div> | </div> | ||
− | |||
</div> | </div> | ||
<div class="sectionTitle" id="Section 5">Modeling Advising and Support</div> | <div class="sectionTitle" id="Section 5">Modeling Advising and Support</div> | ||
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<div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/d/d4/T--Virginia--sponsors--UVA.png"/></div> | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/d/d4/T--Virginia--sponsors--UVA.png"/></div> | ||
<div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/c/c1/T--Virginia--sponsors--IDT.png"/></div> | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/c/c1/T--Virginia--sponsors--IDT.png"/></div> | ||
− | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/ | + | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/4/41/T--Virginia--images--neb.png"/></div> |
− | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/ | + | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/e/e6/T--Virginia--images--twist.png"/></div> |
− | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/ | + | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/4/41/T--Virginia--images--cvillbiohub.png"/></div> |
− | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/ | + | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/b/b8/T--Virginia--sponsors--addgene.png"/></div> |
− | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/ | + | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/7/75/T--Virginia--sponsors--matlab.png"/></div> |
− | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/ | + | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/9/96/T--Virginia--sponsors--Benchling.png"/></div> |
− | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/ | + | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/6/69/T--Virginia--sponsors--qiagen.png"/></div> |
− | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/ | + | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/f/f5/T--Virginia--sponsors--lucid.png"/></div> |
<div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/d/dc/T--Virginia--sponsors--GenScript.png"/></div> | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/d/dc/T--Virginia--sponsors--GenScript.png"/></div> | ||
</div> | </div> |
Latest revision as of 05:28, 18 December 2020
Index:
Attributions
Team
Advisors
Wetlab Advising and Support
Human Practices Advising and Support
Modeling Advising and Support
Entrepreneurship, Legal and/or Business Advising and Support
And a special thanks to...