Difference between revisions of "Team:IIT Roorkee"

 
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                      Gold Medalist • iGEM IIT Roorkee •
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                      Gold Medalist • iGEM IIT Roorkee •
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       <div class="banner banner-home">
 
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           <h1 class="wiki-h wiki-h1 wiki-white">PYROMANCER</h1>
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           <h1 class="wiki-h wiki-h1 wiki-white">PYOMANCER</h1>
 
           <p class="wiki-p wiki-white" style="font-size: 18px;">
 
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             Novel Antibacterial Protien Complexes
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             Novel Antibacterial Protein Complexes
 
             <br/>
 
             <br/>
 
             for MDR Infections
 
             for MDR Infections
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             <a class="banner-button banner-button-1" href="#animation">
 
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                 Explore More
 
                 Explore More
 
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             <a class="banner-button banner-button-2" href="https://2020.igem.org/Team:IIT_Roorkee/Description">
 
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                 View Project
 
                 View Project
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         <img alt="Pyomancer logo" class="banner-image" src="https://static.igem.org/mediawiki/2020/3/3b/T--IIT_Roorkee--images--images--pyomancer.svg"/>
 
         <img alt="Pyomancer logo" class="banner-image" src="https://static.igem.org/mediawiki/2020/3/3b/T--IIT_Roorkee--images--images--pyomancer.svg"/>
 
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           <h2 class="wiki-h wiki-h2 wiki-white">PYROMANCER</h2>
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           <h2 class="wiki-h wiki-h2 wiki-white">PYOMANCER</h2>
 
           <p class="wiki-p wiki-white">
 
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             Novel Antibacterial Protien Complexes
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             Novel Antibacterial Protein Complexes
 
             for MDR Infections
 
             for MDR Infections
 
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               <span class="wiki-white" style="color: #ffffff">
 
                 Explore More
 
                 Explore More
 
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             </a>
 
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             <a class="banner-button banner-button-2" href="">
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             <a class="banner-button banner-button-2" href="https://2020.igem.org/Team:IIT_Roorkee/Description" style="color: #ffffff">
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               <span class="wiki-white" style="color: #ffffff">
 
                 View Project
 
                 View Project
 
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             <video autoplay="" class="home-video" controls="" muted="" src="https://static.igem.org/mediawiki/2020/5/5d/T--IIT_Roorkee--videos--iGEM_IIT_Roorkee_Promo_Video.mp4"></video>
 
             <video autoplay="" class="home-video" controls="" muted="" src="https://static.igem.org/mediawiki/2020/5/5d/T--IIT_Roorkee--videos--iGEM_IIT_Roorkee_Promo_Video.mp4"></video>
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            <div class="wiki-graphic-reference" style="text-align: center;">Project Promotion Video</div>
 
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               In the next few decades, when the bacterial infections would become untamed, we are likely to witness increased death rates by ten times and indefinite damage on the global economy compared to the chaos caused due to the current Pandemic. Subsequently, Hospital Acquired Infections are the leading cause of most of the ICU deaths, and it's imperative to build alternative solutions to treat Bacterial infections and break the wall of antibiotic dependence.
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               This project is aimed at creating novel antibacterial protein complexes, designed to specifically bind and kill species of multidrug-resistant bacteria. This would be achieved by the fusion of antibacterial proteins called pyocins and bacteriophage tail fibers. Furthermore, it aims to create a system for the easy production of such antimicrobials, consisting of an expression plasmid and software. Validation will be done experimentally, targeting the pathogen <i>Acinetobacter baumannii</i>, and production will be done in <i>E. coli</i>.
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              Coming to the rescue, our project provides a narrow-spectrum treatment for the Multi-Drug Resistant Bacteria, A. baumannii, by engineering naturally found elements, namely, Bacteriophage and Pyocins. Pyocins, as produced by P. aeruginosa, provide single-hit killing kinetics. Our key engineering is the design of a novel fusion protein, called Seekercin, which is made by combining the Pyocins and Bacteriophage Tail fiber and expressed in E.coli by the expression plasmid cassette. Essentially we use the natural bactericidal properties of a Pyocin and combine them with the specificity of a page tail. The R-type Pyocin shows a single hit bactericidal activity using a hollow tube, and the AP-22 Tail Fibres of Bacteriophage allow it to attach to the A. baumannii's surface proteins called Lipooligosaccharides.
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              Thus, using synthetic biology and genetic engineering tools, we are designing protein therapies, which on further development can change the way current healthcare systems work. Through our project Pyomancer, we are propagating that it's time for the pharmaceutical industry to make a paradigm shift to narrow-spectrum antimicrobial therapies and regulate the damage done by the continuous use of broad-spectrum antibiotics.
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                 <img alt="Gene detection" class="feature-card-image" src="https://static.igem.org/mediawiki/2020/a/ab/T--IIT_Roorkee--images--images--home-card-1.svg"/>
 
                 <img alt="Gene detection" class="feature-card-image" src="https://static.igem.org/mediawiki/2020/a/ab/T--IIT_Roorkee--images--images--home-card-1.svg"/>
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                 <img alt="Gene detection" class="feature-card-image" src="https://static.igem.org/mediawiki/2020/a/a9/T--IIT_Roorkee--images--images--home-card-2.svg"/>
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                 <img alt="Gene detection" class="feature-card-image" src="https://static.igem.org/mediawiki/2020/9/9d/T--IIT_Roorkee--images--images--home-card-3.svg"/>
 
                 <img alt="Gene detection" class="feature-card-image" src="https://static.igem.org/mediawiki/2020/9/9d/T--IIT_Roorkee--images--images--home-card-3.svg"/>
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                   Click here to understand the concept and working of our software TAILSCOUT, which will enable other researchers to build new Seekercins for other bacterial species in one go!              </p>
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                   Click here to understand the concept and working of our software TailScout, which will enable other researchers to build new Seekercins for other bacterial species in one go!              </p>
 
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                 <img alt="Gene detection" class="feature-card-image" src="https://static.igem.org/mediawiki/2020/b/b5/T--IIT_Roorkee--images--images--home-card-4.svg"/>
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          <a class="wiki-link-button" href="https://2020.igem.org/Team:IIT_Roorkee/Poster">Poster</a>
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Latest revision as of 17:20, 18 December 2020

<!DOCTYPE html> PYOMANCER

Gold Medalist • iGEM IIT Roorkee •
Gold medal
Gold Medalist • iGEM IIT Roorkee •
Gold medal
Project
Project Promotion Video


ABSTRACT

This project is aimed at creating novel antibacterial protein complexes, designed to specifically bind and kill species of multidrug-resistant bacteria. This would be achieved by the fusion of antibacterial proteins called pyocins and bacteriophage tail fibers. Furthermore, it aims to create a system for the easy production of such antimicrobials, consisting of an expression plasmid and software. Validation will be done experimentally, targeting the pathogen Acinetobacter baumannii, and production will be done in E. coli.



The animation explains
the working
mechanism of our
fusion protein.

Pyomancer mechanish GIF



Gene detection

Machine Learning

The year 2020 provided us with an opportunity to explore new areas and bring them to the iGEM community. Dive in to see how we combine ML with synthetic biology and contribute to AMR research.

Gene detection

Bioinformatics

Our protein design involves bioinformatics based prediction and characterization of tail fibers. Check out how we have deployed interesting biophysical modeling techniques to study and characterize our engineered protein.

Gene detection

Software

Click here to understand the concept and working of our software TailScout, which will enable other researchers to build new Seekercins for other bacterial species in one go!

Gene detection

Human Practices

Understanding the ground level complexities of the Hospital Acquired Infections is important. So, Check out the insights we got and the awareness initiatives we undertook for Antibiotic Use Sensitization & Open Science Communication.




Poster