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| − | }</style><header class="d-flex justify-content-center"><div class="custom-container"><div id="header-background"></div><div id="heading"><h1>Atmosphere Regulated Kill Switch</h1></div><a href="https://freepik.com" rel="noopener" target="_blank"><span data-placement="top" id="bg-attribution" title="Photo from Freepik"></span></a></div></header><main class="theme-journal"><div class="custom-container"><div class="row"><div class="content fullwidth"><article><div class="accordion" id="collapsibleSections"><div class="card"><div class="card-header" id="headingOne"><h2 class="mb-0"><button aria-controls="collapseOne" aria-expanded="true" class="btn collapsed" data-target="#collapseOne" data-toggle="collapse" type="button"><span>FNR Characterisation</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingOne" class="collapse" id="collapseOne"><div class="card-body"><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/7/78/T--BITSPilani-Goa_India--docs--Experiments--Killswitch--FNR_Characterisation.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/7/78/T--BITSPilani-Goa_India--docs--Experiments--Killswitch--FNR_Characterisation.pdf" title="FNR Characterisation" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingTwo"><h2 class="mb-0"><button aria-controls="collapseTwo" aria-expanded="true" class="btn collapsed" data-target="#collapseTwo" data-toggle="collapse" type="button"><span>pH Dependency of FNR Promoter</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingTwo" class="collapse" id="collapseTwo"><div class="card-body"><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/9/9f/T--BITSPilani-Goa_India--docs--Experiments--Killswitch--pH_Dependency.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/9/9f/T--BITSPilani-Goa_India--docs--Experiments--Killswitch--pH_Dependency.pdf" title="pH Dependency of FNR Promoter" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingThree"><h2 class="mb-0"><button aria-controls="collapseThree" aria-expanded="true" class="btn collapsed" data-target="#collapseThree" data-toggle="collapse" type="button"><span> | + | }</style><header class="d-flex justify-content-center"><div class="custom-container"><div id="header-background"></div><div id="heading"><h1>Atmosphere Regulated Kill Switch</h1></div><a href="https://freepik.com" rel="noopener" target="_blank"><span data-placement="top" id="bg-attribution" title="Photo from Freepik"></span></a></div></header><main class="theme-journal"><div class="custom-container"><div class="row"><div class="content fullwidth"><article><div class="accordion" id="collapsibleSections"><div class="card"><div class="card-header" id="headingOne"><h2 class="mb-0"><button aria-controls="collapseOne" aria-expanded="true" class="btn collapsed" data-target="#collapseOne" data-toggle="collapse" type="button"><span>FNR Characterisation</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingOne" class="collapse" id="collapseOne"><div class="card-body"><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/7/78/T--BITSPilani-Goa_India--docs--Experiments--Killswitch--FNR_Characterisation.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/7/78/T--BITSPilani-Goa_India--docs--Experiments--Killswitch--FNR_Characterisation.pdf" title="FNR Characterisation" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingTwo"><h2 class="mb-0"><button aria-controls="collapseTwo" aria-expanded="true" class="btn collapsed" data-target="#collapseTwo" data-toggle="collapse" type="button"><span>pH Dependency of FNR Promoter</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingTwo" class="collapse" id="collapseTwo"><div class="card-body"><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/9/9f/T--BITSPilani-Goa_India--docs--Experiments--Killswitch--pH_Dependency.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/9/9f/T--BITSPilani-Goa_India--docs--Experiments--Killswitch--pH_Dependency.pdf" title="pH Dependency of FNR Promoter" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingThree"><h2 class="mb-0"><button aria-controls="collapseThree" aria-expanded="true" class="btn collapsed" data-target="#collapseThree" data-toggle="collapse" type="button"><span>Kill switch Response Time Determination</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingThree" class="collapse" id="collapseThree"><div class="card-body"><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/a/ab/T--BITSPilani-Goa_India--docs--Experiments--Killswitch--Response_Time.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/a/ab/T--BITSPilani-Goa_India--docs--Experiments--Killswitch--Response_Time.pdf" title="Killswitch Response Time Determination" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingFive"><h2 class="mb-0"><button aria-controls="collapseFour" aria-expanded="true" class="btn collapsed" data-target="#collapseFour" data-toggle="collapse" type="button"><span>Electrophoretic Mobility Shift Assay</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingFour" class="collapse" id="collapseFour"><div class="card-body"><p>To find the K<sub>d</sub> value of the FNR promoter, we propose to use the following protocol.</p><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/4/4c/T--BITSPilani-Goa_India--docs--Experiments--Protocols--EMSA.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/4/4c/T--BITSPilani-Goa_India--docs--Experiments--Protocols--EMSA.pdf" title="Electrophoretic Mobility Shift Assay" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingFive"><h2 class="mb-0"><button aria-controls="collapseFive" aria-expanded="true" class="btn collapsed" data-target="#collapseFive" data-toggle="collapse" type="button"><span>Site Directed Mutagenesis</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingFive" class="collapse" id="collapseFive"><div class="card-body"><p>To tune the K<sub>d</sub> value of the FNR promoter, we propose to use the following protocol.</p><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/a/ae/T--BITSPilani-Goa_India--docs--Experiments--Protocols--Site_Directed_Mutagenesis.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/a/ae/T--BITSPilani-Goa_India--docs--Experiments--Protocols--Site_Directed_Mutagenesis.pdf" title="Site Directed Mutagenesis" type="application/pdf"/></div></div></div></div></article><div id="buttons"><a class="button prev" href="https://2020.igem.org/Team:BITSPilani-Goa_India/Experiments/pFruB-Cra_System"><span class="button-background"><span aria-hidden="" class="circle"><span class="icon arrow"></span></span></span><span class="button-text">pFruB-Cra System</span></a><a class="button next" href="https://2020.igem.org/Team:BITSPilani-Goa_India/Experiments/Inoculant"><span class="button-background"><span aria-hidden="" class="circle"><span class="icon arrow"></span></span></span><span class="button-text">Inoculant</span></a></div></div></div></div></main><footer><div id="footerNav"><div 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To find the Kd value of the FNR promoter, we propose to use the following protocol.
To download this document, click here.
To tune the Kd value of the FNR promoter, we propose to use the following protocol.
To download this document, click here.