Difference between revisions of "Team:Virginia/Protocols"

Revision as of 05:07, 27 October 2020

Manifold

MANIFOLD

Index:

Lab Notebook

Introduction

Below are our Benchling Protocol pages that show in detail the protocols we utilized in the construction of MANIFOLD. For more details on how these protocols were utilized, see the Notebook page. For information on the planned use of these protocols, see the Experiments page.

Protocols

Agarose Gel Creation and Pour

Agarose Gel Electrophoresis

Culture from Agar Stab (Inoculation)

DpnI Incubation

Glycerol Stock

Golden Gate Assembly with BsaI

Golden Gate Assembly with BsmBI

NEB Time-Saver qualified Restriction Enzyme Digestion

NEBuilder Hifi Mutagenesis

PCR Purification Kit (GenScript)

Phusion PCR Protocol

QIAGEN HiSpeed Plasmid Purification

QIAprep Spin Miniprep Kit

Restriction Enzyme(s) Digest

Site Directed Mutagenesis

Standard Assembly (Column 3 - pduD + GFP)

Standard Assembly (Combining Column 1 and 3)

Transforming Competent Cells

TRIzol DNA Extraction

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                <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia">Main</a>
                <a class="hvr-sweep-to-right" href="#abstract">Abstract</a>
-              <a class="hvr-sweep-to-right" href="#problem">Problem</a>
-              <a class="hvr-sweep-to-right" href="#solution">Solution</a>
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              <a class="mainitem" href="#project">PROJECT</a>
              <div class="dropdown-content">
-              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Inspiration">Inspiration</a>
                <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Description">Description</a>
                <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Design">Design</a>
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                <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Engineering">Engineering</a>
                <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Implementation">Implementation</a>
-              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Device">Device</a>
                <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Notebook">Notebook</a>
                <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Safety">Safety</a>
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              <a class="mainitem" href="#parts">PARTS</a>
              <div class="dropdown-content">
-               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/New_parts">New Parts</a>
+               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/New_parts"> Parts</a>
-              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Utilized_parts">Utilized Parts</a>
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                <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Members">Members</a>
                <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Attributions">Attributions</a>
-              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Gallery">Gallery</a>
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