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<div class="title"> Inspiration </div> | <div class="title"> Inspiration </div> | ||
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− | <div class="text">Cadmium contamination of water worldwide is already having a severe environmental impact, especially on crops, such as cadmium rice. In addition, cadmium contamination is likely to cause itai-itai disease and renal tubular injury, after prolonged and/or high exposure may eventually to renal failure, thus, the problem of cadmium pollution of water needs to be solved urgently. We adopt an environmentally friendly biological approach——use Synechocystis sp. PCC6803 with a clear genetic background and a certain degree of tolerance to cadmium as a chassis organism for transformation. </ | + | <div class="text"> |
− | + | <p>Cadmium contamination of water worldwide is already having a severe environmental impact, especially on crops, such as cadmium rice. In addition, cadmium contamination is likely to cause itai-itai disease and renal tubular injury, after prolonged and/or high exposure may eventually to renal failure, thus, the problem of cadmium pollution of water needs to be solved urgently. We adopt an environmentally friendly biological approach——use Synechocystis sp. PCC6803 with a clear genetic background and a certain degree of tolerance to cadmium as a chassis organism for transformation.</p> | |
− | + | <p>First of all, we translate cadmium, which is enriched on the cell wall through transporter proteins. However, this may cause photosynthesis failure, protein inactivation or oxidative stress in Synechocystis and lead to cell death, making it difficult to achieve high tolerance rate. Therefore, after transporting large amounts of cadmium ions into the cells, we accumulate heavy metal ions and prevent them from being transported out of the cells by chelating proteins. At the same time, we increase the expression of oxidoreductase to remove reactive oxygen clusters in the cells, thus increasing the growth rate of algae under cadmium ion stress. Besides, for high expression of functional proteins, We optimized the codon preferences of the algae, and analyzed the protein expression results.(Figure 1)</p> | |
− | + | <img src="https://static.igem.org/mediawiki/2020/3/3e/T--CSU_CHINA--Figure1-design.jpeg" /> | |
+ | <div style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Figure1: The core “transport-chelation-redox reaction” system of our project</div> | ||
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Revision as of 16:13, 10 November 2020
Poster: CSU_CHINA
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Cadmium contamination of water worldwide is already having a severe environmental impact, especially on crops, such as cadmium rice. In addition, cadmium contamination is likely to cause itai-itai disease and renal tubular injury, after prolonged and/or high exposure may eventually to renal failure, thus, the problem of cadmium pollution of water needs to be solved urgently. We adopt an environmentally friendly biological approach——use Synechocystis sp. PCC6803 with a clear genetic background and a certain degree of tolerance to cadmium as a chassis organism for transformation.
First of all, we translate cadmium, which is enriched on the cell wall through transporter proteins. However, this may cause photosynthesis failure, protein inactivation or oxidative stress in Synechocystis and lead to cell death, making it difficult to achieve high tolerance rate. Therefore, after transporting large amounts of cadmium ions into the cells, we accumulate heavy metal ions and prevent them from being transported out of the cells by chelating proteins. At the same time, we increase the expression of oxidoreductase to remove reactive oxygen clusters in the cells, thus increasing the growth rate of algae under cadmium ion stress. Besides, for high expression of functional proteins, We optimized the codon preferences of the algae, and analyzed the protein expression results.(Figure 1)