Difference between revisions of "Team:CSU CHINA/Poster"

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<div class="title">transportation - chelate - redox</div>
 
<div class="title">transportation - chelate - redox</div>
 
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<p><span style="font-weight: bolder">Cadmium contamination</span> of water worldwide is already having a severe environmental impact, especially on crops, such as cadmium rice. In addition, cadmium contamination is likely to cause itai-itai disease and renal tubular injury, after prolonged and/or high exposure may eventually to renal failure, thus, the problem of cadmium pollution of water needs to be solved urgently. We adopt an environmentally friendly biological approach——use <span style="font-weight: bolder">Synechocystis sp. PCC6803</span> with a clear genetic background and a certain degree of tolerance to cadmium as a chassis organism for transformation.</p>
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<p>First of all, we <span style="font-weight: bolder">translate</span> cadmium, which is enriched on the cell wall through transporter proteins. However, this may cause photosynthesis failure, protein inactivation or oxidative stress in Synechocystis and lead to cell death, making it difficult to achieve high tolerance rate. Therefore, after transporting large amounts of cadmium ions into the cells, we accumulate heavy metal ions and prevent them from being transported out of the cells by <span style="font-weight: bolder">chelating proteins</span>. At the same time, we increase the expression of <span style="font-weight: bolder">oxidoreductase</span> to remove reactive oxygen clusters in the cells, thus increasing the growth rate of algae under cadmium ion stress. Besides, for high expression of functional proteins, We optimized the codon preferences of the algae, and analyzed the protein expression results.(Figure 1)</p>
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<img src="https://static.igem.org/mediawiki/2020/3/3e/T--CSU_CHINA--Figure1-design.jpeg" />
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<div style="width: 80%;text-align:center;font-size: .9rem; margin: 0.5rem auto 1rem auto; color: #888;">Figure1: The core “transport-chelation-redox reaction” system of our project</div>
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<p>We extended module level from cell into the real world through this model. Based on We extended module level from cell into the real world through this model. Based on element analysis, we rebuilt the microbead and cells virtually, and predict its reaction, then get the module optimized.</p>
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<img src="https://static.igem.org/mediawiki/2020/c/c6/T--CSU_CHINA--MN_fig1.png">
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<p style="width: 80%;text-align:center;font-size: .9rem; margin: 0.5rem auto 1rem auto; color: #888;">Model 1: \( {Cd}^{2+} \)  flow between environment and microbead</p>
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<img src="https://static.igem.org/mediawiki/2020/0/04/T--CSU_CHINA--Am_fig1.gif">
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<p style="width: 80%;text-align:center;font-size: .9rem; margin: 0.5rem auto 1rem auto; color: #888;">Model 2: \( {Cd}^{2+} \) flow between algae and microbead</p>
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<div class="title"> Section 1 </div>
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<div class="text"> Use this section to explain whatever you would like! Suggestions: Safety, Human Practices, Measurement, etc. </div>
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<div class="title"> "Toxin-antitoxin" light-controlled suicide system </div>
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<p>The <span style="font-weight: bolder">escape of engineered synechocystis</span> is one of the major hazards of genetically modified organism, which may lead to different adverse effects such as <span style="font-weight:bolder">water bloom</span> and reduction of water polymorphism. Considering these risks, we designed a <span style="font-weight: bolder">blue-light-controlled "toxin-antitoxin" suicide system</span>. (Figure 2) In this system we use the <span style="font-weight: bolder">RelE/RelB</span>-toxin/antitoxin system and regulate the expression of the toxin RelE through the blue light-regulated promoter. In the presence of blue light, the regularly expressed HTH-LOV-REP blue-regulated protein will homodimerize and specifically recognize the (c120)5 sequence upstream of the blue-regulated promoter via HTH, while inhibiting the expression of toxin RelE via the REP domain. When synechocystis escaped from the blue light environment we have set up, the uninhibited blue light regulatory promoter will initiate the expression of RelE and under the effect of our amplification system, the content of RelE will increase rapidly, as a result the escaped algae will be killed. Meanwhile in order to prevent the small amount of RelE that is still expressed in the presence of blue light from harming the algae, we regularly express the antitoxin <span style="font-weight: bolder">RelB</span> to neutralize its toxin to ensure that the toxin RelE does not harm the unescaped Synechocystis sp.PCC6803.</p>
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<img src="https://static.igem.org/mediawiki/2020/9/93/T--CSU_CHINA--Figure2-design.jpeg" />
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<p style="width: 80%;text-align:center;font-size: .9rem; margin: 0.5rem auto 1rem auto; color: #888;">Figure 2:Third-generation system the Blue light regulated suicide system</p>
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<div class="text"> Use this section to explain whatever you would like! Suggestions: Safety, Human Practices, Measurement, etc. </div>
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<div class="text"> What did your team achieve? What do you plan to work on moving forward? </div>
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<div class="text">If not already cited in other sections of your poster, what literature sources did you reference on this poster? Who helped or advised you? </div>
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Revision as of 16:40, 10 November 2020

Poster: CSU_CHINA



Poster Template

This poster template will let you create an interactive poster! The poster is divided in two parts: the visual overview on the left and the documentation on the right. The visual overview is broken down into sections that the user can click on. When a user clicks on a poster section in the visual overview, the documentation on the right will display the text and graphics associated with this section of the poster. You can find the documentation on how to use this template, as well as an example here: https://2020.igem.org/Competition/Deliverables/Poster

Poster Title
You should list all authors and their affiliations here. You can also add the project's abstract.
Introduction
Introduce your project and your team's goals.
Inspiration

Cadmium contamination of water worldwide is already having a severe environmental impact, especially on crops, such as cadmium rice. In addition, cadmium contamination is likely to cause itai-itai disease and renal tubular injury, after prolonged and/or high exposure may eventually to renal failure, thus, the problem of cadmium pollution of water needs to be solved urgently. We adopt an environmentally friendly biological approach——use Synechocystis sp. PCC6803 with a clear genetic background and a certain degree of tolerance to cadmium as a chassis organism for transformation.


First of all, we translate cadmium, which is enriched on the cell wall through transporter proteins. However, this may cause photosynthesis failure, protein inactivation or oxidative stress in Synechocystis and lead to cell death, making it difficult to achieve high tolerance rate. Therefore, after transporting large amounts of cadmium ions into the cells, we accumulate heavy metal ions and prevent them from being transported out of the cells by chelating proteins. At the same time, we increase the expression of oxidoreductase to remove reactive oxygen clusters in the cells, thus increasing the growth rate of algae under cadmium ion stress. Besides, for high expression of functional proteins, We optimized the codon preferences of the algae, and analyzed the protein expression results.(Figure 1)


Figure1: The core “transport-chelation-redox reaction” system of our project
Problem
What is the problem your team is working to solve? How does it affect the world?
Idea
How are you going to solve the problem? Where did the idea come from?
Engineering
Here you can explain the engineering process behind your project.
Section 1
Use this section to explain whatever you would like! Suggestions: Safety, Human Practices, Measurement, etc.
transportation - chelate - redox

Cadmium contamination of water worldwide is already having a severe environmental impact, especially on crops, such as cadmium rice. In addition, cadmium contamination is likely to cause itai-itai disease and renal tubular injury, after prolonged and/or high exposure may eventually to renal failure, thus, the problem of cadmium pollution of water needs to be solved urgently. We adopt an environmentally friendly biological approach——use Synechocystis sp. PCC6803 with a clear genetic background and a certain degree of tolerance to cadmium as a chassis organism for transformation.


First of all, we translate cadmium, which is enriched on the cell wall through transporter proteins. However, this may cause photosynthesis failure, protein inactivation or oxidative stress in Synechocystis and lead to cell death, making it difficult to achieve high tolerance rate. Therefore, after transporting large amounts of cadmium ions into the cells, we accumulate heavy metal ions and prevent them from being transported out of the cells by chelating proteins. At the same time, we increase the expression of oxidoreductase to remove reactive oxygen clusters in the cells, thus increasing the growth rate of algae under cadmium ion stress. Besides, for high expression of functional proteins, We optimized the codon preferences of the algae, and analyzed the protein expression results.(Figure 1)


Figure1: The core “transport-chelation-redox reaction” system of our project
Section 3
Use this section to explain whatever you would like! Suggestions: Safety, Human Practices, Measurement, etc.
Results
What did your team achieve? What do you plan to work on moving forward?
References and Acknowledgements

We extended module level from cell into the real world through this model. Based on We extended module level from cell into the real world through this model. Based on element analysis, we rebuilt the microbead and cells virtually, and predict its reaction, then get the module optimized.


Model 1: \( {Cd}^{2+} \) flow between environment and microbead

Model 2: \( {Cd}^{2+} \) flow between algae and microbead

Poster Title
You should list all authors and their affiliations here. You can also add the project's abstract.
Section 1
Use this section to explain whatever you would like! Suggestions: Safety, Human Practices, Measurement, etc.
"Toxin-antitoxin" light-controlled suicide system

The escape of engineered synechocystis is one of the major hazards of genetically modified organism, which may lead to different adverse effects such as water bloom and reduction of water polymorphism. Considering these risks, we designed a blue-light-controlled "toxin-antitoxin" suicide system. (Figure 2) In this system we use the RelE/RelB-toxin/antitoxin system and regulate the expression of the toxin RelE through the blue light-regulated promoter. In the presence of blue light, the regularly expressed HTH-LOV-REP blue-regulated protein will homodimerize and specifically recognize the (c120)5 sequence upstream of the blue-regulated promoter via HTH, while inhibiting the expression of toxin RelE via the REP domain. When synechocystis escaped from the blue light environment we have set up, the uninhibited blue light regulatory promoter will initiate the expression of RelE and under the effect of our amplification system, the content of RelE will increase rapidly, as a result the escaped algae will be killed. Meanwhile in order to prevent the small amount of RelE that is still expressed in the presence of blue light from harming the algae, we regularly express the antitoxin RelB to neutralize its toxin to ensure that the toxin RelE does not harm the unescaped Synechocystis sp.PCC6803.


Figure 2:Third-generation system the Blue light regulated suicide system

Section 3
Use this section to explain whatever you would like! Suggestions: Safety, Human Practices, Measurement, etc.
Results
What did your team achieve? What do you plan to work on moving forward?
References and Acknowledgements
If not already cited in other sections of your poster, what literature sources did you reference on this poster? Who helped or advised you?
Poster Title
You should list all authors and their affiliations here. You can also add the project's abstract.

t explaining this section -->

Cadmium contamination of water worldwide is already having a severe environmental impact, especially on crops, such as cadmium rice. In addition, cadmium contamination is likely to cause itai-itai disease and renal tubular injury, after prolonged and/or high exposure may eventually to renal failure, thus, the problem of cadmium pollution of water needs to be solved urgently. We adopt an environmentally friendly biological approach——use Synechocystis sp. PCC6803 with a clear genetic background and a certain degree of tolerance to cadmium as a chassis organism for transformation.


First of all, we translate cadmium, which is enriched on the cell wall through transporter proteins. However, this may cause photosynthesis failure, protein inactivation or oxidative stress in Synechocystis and lead to cell death, making it difficult to achieve high tolerance rate. Therefore, after transporting large amounts of cadmium ions into the cells, we accumulate heavy metal ions and prevent them from being transported out of the cells by chelating proteins. At the same time, we increase the expression of oxidoreductase to remove reactive oxygen clusters in the cells, thus increasing the growth rate of algae under cadmium ion stress. Besides, for high expression of functional proteins, We optimized the codon preferences of the algae, and analyzed the protein expression results.(Figure 1)


Figure1: The core “transport-chelation-redox reaction” system of our project
Section 3
Use this section to explain whatever you would like! Suggestions: Safety, Human Practices, Measurement, etc.
Results
What did your team achieve? What do you plan to work on moving forward?
References and Acknowledgements

We extended module level from cell into the real world through this model. Based on We extended module level from cell into the real world through this model. Based on element analysis, we rebuilt the microbead and cells virtually, and predict its reaction, then get the module optimized.


Model 1: flow between environment and microbead

Model 2: flow between algae and microbead

Poster Title
You should list all authors and their affiliations here. You can also add the project's abstract.
Section 1
Use this section to explain whatever you would like! Suggestions: Safety, Human Practices, Measurement, etc.
"Toxin-antitoxin" light-controlled suicide system

The escape of engineered synechocystis is one of the major hazards of genetically modified organism, which may lead to different adverse effects such as water bloom and reduction of water polymorphism. Considering these risks, we designed a blue-light-controlled "toxin-antitoxin" suicide system. (Figure 2) In this system we use the RelE/RelB-toxin/antitoxin system and regulate the expression of the toxin RelE through the blue light-regulated promoter. In the presence of blue light, the regularly expressed HTH-LOV-REP blue-regulated protein will homodimerize and specifically recognize the (c120)5 sequence upstream of the blue-regulated promoter via HTH, while inhibiting the expression of toxin RelE via the REP domain. When synechocystis escaped from the blue light environment we have set up, the uninhibited blue light regulatory promoter will initiate the expression of RelE and under the effect of our amplification system, the content of RelE will increase rapidly, as a result the escaped algae will be killed. Meanwhile in order to prevent the small amount of RelE that is still expressed in the presence of blue light from harming the algae, we regularly express the antitoxin RelB to neutralize its toxin to ensure that the toxin RelE does not harm the unescaped Synechocystis sp.PCC6803.


Figure 2:Third-generation system the Blue light regulated suicide system

Section 3
Use this section to explain whatever you would like! Suggestions: Safety, Human Practices, Measurement, etc.
Results
What did your team achieve? What do you plan to work on moving forward?
References and Acknowledgements
If not already cited in other sections of your poster, what literature sources did you reference on this poster? Who helped or advised you?
Poster Title
You should list all authors and their affiliations here. You can also add the project's abstract.