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| <div class="row"> | | <div class="row"> |
| <!-- This creates a full width section with the id 'quaranskin' ---> | | <!-- This creates a full width section with the id 'quaranskin' ---> |
| + | <!-- Quaranskin --> |
| <div class="section two_thirds" id="quaranskin"> | | <div class="section two_thirds" id="quaranskin"> |
| <img src="https://static.igem.org/mediawiki/2020/5/5b/T--Paris_Bettencourt--Poster_Quaranskin51.jpg"> | | <img src="https://static.igem.org/mediawiki/2020/5/5b/T--Paris_Bettencourt--Poster_Quaranskin51.jpg"> |
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| src="https://static.igem.org/mediawiki/2020/1/11/T--Paris_Bettencourt--Poster_QuaranskinStudy2.png"> | | src="https://static.igem.org/mediawiki/2020/1/11/T--Paris_Bettencourt--Poster_QuaranskinStudy2.png"> |
| <p><b>Participants recruitment</b></p> | | <p><b>Participants recruitment</b></p> |
− | <p>Emails are sent to all the members of our institution, the CRI, and all the european iGEMers, by focusing on people form countries where confinement or social distanciation are set up. The total number of participants expected is 70. | + | <p>Emails are sent to all the members of our institution, the CRI, and all the european |
| + | iGEMers, by focusing on people form countries where confinement or social distanciation |
| + | are set up. The total number of participants expected is 70. |
| </p> | | </p> |
| <p><b>Participants enrollment</b></p> | | <p><b>Participants enrollment</b></p> |
− | <p>To be involved in the study each participants has to fill in a participation form and link to it a signed and dated consent form. | + | <p>To be involved in the study each participants has to fill in a participation form and |
| + | link to it a signed and dated consent form. |
| </p> | | </p> |
− | <p>A link is sent to them allowing them to create a account on the OpenHumans platform in order to furnish them an ID code which we use to keep anonymity | + | <p>A link is sent to them allowing them to create a account on the OpenHumans platform in |
| + | order to furnish them an ID code which we use to keep anonymity |
| </p> | | </p> |
| <p><b>Participants action</b></p> | | <p><b>Participants action</b></p> |
− | <p>Once they are officially enroll in the study, we send to the participants by mail a kit containing all tools needed to sample microbiome from 4 body sites and to send them back to us, and by email a link to answer an online questionnaire asking questions covering 4 main topics : The intrinsic characteristic (age, sexe, nationality...), the hygiene habits, the level of confinement and the skin disorder appeared since February 2020. | + | <p>Once they are officially enroll in the study, we send to the participants by mail a kit |
| + | containing all tools needed to sample microbiome from 4 body sites and to send them back |
| + | to us, and by email a link to answer an online questionnaire asking questions covering 4 |
| + | main topics : The intrinsic characteristic (age, sexe, nationality...), the hygiene |
| + | habits, the level of confinement and the skin disorder appeared since February 2020. |
| </p> | | </p> |
| <p><b>Microbiome sequencing</b></p> | | <p><b>Microbiome sequencing</b></p> |
− | <p>Once we receive the microbiome samples we send them directly to an external company, Genewiz. They’ll extract bacterial DNA from the samples, amplify the V3-V4 regions of the 16S RNA gene, and then sequence the amplicons. | + | <p>Once we receive the microbiome samples we send them directly to an external company, |
| + | Genewiz. They’ll extract bacterial DNA from the samples, amplify the V3-V4 regions of |
| + | the 16S RNA gene, and then sequence the amplicons. |
| </p> | | </p> |
| <p><b>Statistical analysis</b></p> | | <p><b>Statistical analysis</b></p> |
− | <p>After having analyzed the composition and diversity of each microbiome we’ll link these results to the answer to the questionnaire to finally find some correlations between some microbiome compositions and some characteristics of the lifestyle or some skin disorder | + | <p>After having analyzed the composition and diversity of each microbiome we’ll link these |
| + | results to the answer to the questionnaire to finally find some correlations between |
| + | some microbiome compositions and some characteristics of the lifestyle or some skin |
| + | disorder |
| </p> | | </p> |
| </div> | | </div> |
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| | | |
| <!-- This creates a full width section with the id 'Implementation' ---> | | <!-- This creates a full width section with the id 'Implementation' ---> |
| + | <!-- Implementation --> |
| <div class="section third" id="Implementation"> | | <div class="section third" id="Implementation"> |
| <img src="https://static.igem.org/mediawiki/2020/9/9b/T--Paris_Bettencourt--Poster_Implementation52.jpg"> | | <img src="https://static.igem.org/mediawiki/2020/9/9b/T--Paris_Bettencourt--Poster_Implementation52.jpg"> |
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| <div class="info"> | | <div class="info"> |
| <!--Write the title of the section --> | | <!--Write the title of the section --> |
− | <div class="title"> Engineering</div> | + | <div class="title">Implementation</div> |
| <!--Write the text explaining this section --> | | <!--Write the text explaining this section --> |
− | <div class="text"> Here you can explain the engineering process behind your project. </div> | + | <div class="text"> |
| + | <p><b class="heading">Ethical consideration</b></p> |
| + | <p>As Quaranskin is a collection of data extracted from human derived samples (skin |
| + | microbiome) and personal information (answer to the questionnaire), it’s important to |
| + | learn about the ethical rules that govern research involving human being in Europe in |
| + | order to respect the protection of the participants in our study. |
| + | </p> |
| + | <p>In France, Research Involving Human Being (RIPH) has to be approved and framed by the |
| + | Committees for the Protection of Persons (CPP). |
| + | </p> |
| + | <p>We have submitted to the ethics committee a complete file presenting our study in detail. |
| + | This file consisted mainly of a research protocol and all the information documents for |
| + | the study participants. Even though we already recruit participant and set up all the |
| + | logistic aspect of the study, we are still waiting for their approval to collect and |
| + | analyse samples. |
| + | </p> |
| + | |
| + | <p><b class="heading">Contribution to the scientific community</b></p> |
| + | <ol> |
| + | <li>Increase the knowledge we have about the microbiome and more precisely about the |
| + | external factors that can impact it. |
| + | </li> |
| + | <li>Create an open database of skin microbiome from people in the context of a pandemic. |
| + | </li> |
| + | <li>Develop Science@Home by providing pipeline and protocols useful for future |
| + | microbiome studies based on citizen science |
| + | </li> |
| + | <li>Instruct participants about their microbiome by sending them back their microbiome |
| + | profile after analysis. |
| + | </li> |
| + | </ol> |
| + | </div> |
| </div> | | </div> |
| </div> | | </div> |