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− | https://static.igem.org/mediawiki/2020/b/b0/T--Paris_Bettencourt--Poster_EpiFlexLogo.png | + | {{Poster}} |
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| + | <html> |
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| + | <style> |
| + | #section_title { |
| + | color: #ff0000; |
| + | background-color: #fff5efff; |
| + | text-align: center; |
| + | padding: 8px; |
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| + | #section_text { |
| + | padding: 8px; |
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| + | p { |
| + | color: #003366 !important; |
| + | text-align: justify; |
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| + | text-align: center; |
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| + | </style> |
| + | |
| + | <div class="page_wrapper"> |
| + | <div class="page_center"> |
| + | |
| + | |
| + | <!-- <div style="padding: 20px 0px; width:95%; margin:auto; font-size:14px;"> |
| + | <h1> Poster: Paris_Bettencourt </h1> |
| + | <br><br> |
| + | |
| + | <h3> Poster Template </h3> |
| + | <p> This poster template will let you create an interactive poster! The poster is divided in two parts: the |
| + | visual overview on the left and the documentation on the right. The visual overview is broken down into |
| + | sections that the user can click on. When a user clicks on a poster section in the visual overview, the |
| + | documentation on the right will display the text and graphics associated with this section of the |
| + | poster. You can find the documentation on how to use this template, as well as an example here: <a |
| + | href="https://2020.igem.org/Competition/Deliverables/Poster">https://2020.igem.org/Competition/Deliverables/Poster</a> |
| + | </p> |
| + | |
| + | |
| + | |
| + | </div> --> |
| + | |
| + | <!---Place the content of your poster inside this div---> |
| + | <div class="poster_w content"> |
| + | |
| + | <!--This is the first row of the poster, it contains the title section ---> |
| + | <!-- Poster title --> |
| + | <div class="row"> |
| + | <!-- This creates a full width section with the id 'title' , since it will be the default section loaded, we add the class 'first_text' ---> |
| + | <div class="section full first_text" id="title"> |
| + | |
| + | <!--Here is the image used for the full width section, the size is: 1200 x 200 pixels --> |
| + | <img src="https://static.igem.org/mediawiki/2020/0/0f/T--Paris_Bettencourt--Poster_Title1.jpg"> |
| + | |
| + | <!--Info: Place the title and text for this section, it will appear when the user clicks on it since the first_text class is used above, this section will appear by default --> |
| + | <div class="info"> |
| + | <!--Write the title of the section --> |
| + | <div class="title">SynDerma - The Skin Microbiome in the Spotlight: <br /> From Sampling to |
| + | Engineering |
| + | </div> |
| + | <!--Write the text explaining this section --> |
| + | <div class="text"> |
| + | <p>Presented by Team Paris Bettencourt 2020</p> |
| + | <br /> |
| + | <p><b>Amandine Maire<sup>1</sup>, Anu Susan Kurian<sup>1</sup>, Chetan Kumar |
| + | Velumurugan<sup>1</sup>, Nicolas Levrier<sup>1</sup>, Nikola Zarevski<sup>1</sup>, |
| + | Valerie March<sup>1</sup>, Xavier Olessa-Daragon<sup>1</sup>, Ariel |
| + | Lindner<sup>2</sup>, Jake Wintermute<sup>2</sup>, Radoslaw Ejsmont<sup>2</sup>, |
| + | Alexis Casas<sup>2</sup>, Darshak Bhatt<sup>2</sup></b></p> |
| + | <br /> |
| + | <p><b><sup>1</sup>Student Team Member</b>, <sup>2</sup>Team Mentor</p> |
| + | <br /> |
| + | <b>Abstract</b> |
| + | <p></p> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | |
| + | |
| + | <!--This is the second row of the poster, it contains the introduction section ---> |
| + | <!-- Objectives --> |
| + | <div class="row"> |
| + | <!-- This creates a full width section with the id 'objective' ---> |
| + | <div class="section full" id="objective"> |
| + | <!--Here is the image used for the full width section, the size is: 1200 x 200 pixels --> |
| + | <img src="https://static.igem.org/mediawiki/2020/9/90/T--Paris_Bettencourt--Poster_Objectives1.jpg"> |
| + | |
| + | <!--Info: Place the title and text for this section, it will appear when the user clicks on it--> |
| + | <div class="info"> |
| + | <!--Write the title of the section --> |
| + | <div class="title"> Objectives </div> |
| + | <!--Write the text explaining this section --> |
| + | <div class="text"> |
| + | <!-- <p><b>Objectives 1.</b></p> --> |
| + | <p><b class="heading">1. Sample and Sequence the Human Skin Microbiome</b></p> |
| + | <p>Our first objective, pursued as part of the Quaranskin project, is to study the impact of |
| + | environmental and behavioral factors on the skin microbiome and to provide a new |
| + | database to study its composition in the context of reduced social and environmental |
| + | interactions. |
| + | </p> |
| + | <br /> |
| + | <!-- <p><b>Objectives 2.</b></p> --> |
| + | <p><b class="heading">2. Design tools to engineer the bacterium <i>S. epidermidis</i></b> |
| + | </p> |
| + | <p>Our second objective is to make S. epidermidis an efficient synthetic biology chassis |
| + | that can be used to monitor the population dynamics of the skin microbiome. This in |
| + | order to maintain its equilibrium and to avoid pathologies induced by dysbiosis. |
| + | </p> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | |
| + | |
| + | <!--This is the third row of the poster, it contains the inspiration, problem and idea sections ---> |
| + | <!-- Inspiration, Challenge, Response --> |
| + | <div class="row"> |
| + | |
| + | <!-- This creates a third width section with the id 'inspiration' ---> |
| + | <!-- Inspiration --> |
| + | <div class="section third" id="inspiration"> |
| + | <!--Here is the image used for the third width section, the size is: 400 x 200 pixels --> |
| + | <img src="https://static.igem.org/mediawiki/2020/1/18/T--Paris_Bettencourt--Poster_Inspiration31.jpg"> |
| + | |
| + | <!--Info: Place the title and text for this section, it will appear when the user clicks on it--> |
| + | <div class="info"> |
| + | |
| + | <!--Write the title of the section --> |
| + | <div class="title"> Inspiration </div> |
| + | <!--Write the text explaining this section --> |
| + | <div class="text"> |
| + | <p>In the context of COVID-19 pandemic, we were, as most of the other iGEM teams confined at |
| + | home.</p> |
| + | |
| + | <div class="subsection third"> |
| + | <img |
| + | src="https://static.igem.org/mediawiki/2020/e/e5/T--Paris_Bettencourt--Poster_Inspiration1.png"> |
| + | </div> |
| + | <div class="subsection two_thirds"> |
| + | <br /> |
| + | <br /> |
| + | <p class="padding-left">As this situation was unprecedented, we were wondering if the |
| + | lockdown could have an |
| + | impact on human health and more specifically on the composition of our skin |
| + | microbiome. |
| + | </p> |
| + | </div> |
| + | |
| + | <div class="clear"></div> |
| + | <br /> |
| + | |
| + | <div class="subsection two_thirds"> |
| + | <p class="padding-right">We met some dermatologists as Stéphanie Leclerc-Mercier. She |
| + | pointed out the fact |
| + | that the new sanitary measures and hygiene habits imposed by the pandemic were a |
| + | real issue for people with eczema symptoms since they cannot wash their hands as |
| + | often as healthy people. This discussion drew our interest for dysbiosis induced |
| + | pathologies and ways to treat them using synthetic biology. |
| + | </p> |
| + | </div> |
| + | <div class="subsection third"> |
| + | <img |
| + | src="https://static.igem.org/mediawiki/2020/0/03/T--Paris_Bettencourt--Poster_Inspiration2.png"> |
| + | </div> |
| + | |
| + | <div class="clear"></div> |
| + | <br /> |
| + | |
| + | <div class="subsection third"> |
| + | <img |
| + | src="https://static.igem.org/mediawiki/2020/6/63/T--Paris_Bettencourt--Poster_Inspiration3.png"> |
| + | </div> |
| + | <div class="subsection two_thirds"> |
| + | <br /> |
| + | <br /> |
| + | <p class="padding-left">Our team personal values led us to create a project who enters |
| + | in |
| + | the framework of |
| + | Open and Citizen Science. Especially during the lockdown which forced us to be |
| + | physically isolated of each other, we wanted to find a way to connect people through |
| + | science. |
| + | </p> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | |
| + | |
| + | <!-- This creates a third width section with the id 'challenge' ---> |
| + | <!-- Challenge --> |
| + | <div class="section third" id="challenges"> |
| + | <!--Here is the image used for the third width section, the size is: 400 x 200 pixels --> |
| + | <img src="https://static.igem.org/mediawiki/2020/7/7e/T--Paris_Bettencourt--Poster_Challenges32.jpg"> |
| + | |
| + | <!--Info: Place the title and text for this section, it will appear when the user clicks on it--> |
| + | <div class="info"> |
| + | |
| + | <!--Write the title of the section --> |
| + | <div class="title"> Challenges </div> |
| + | <!--Write the text explaining this section --> |
| + | <div class="text"> |
| + | <p><b class="heading">1. Study the Skin Microbiome</b></p> |
| + | <p>Human skin microbiome sampling is a challenge in itself since it implies reaching out to |
| + | a |
| + | large cohort and collecting human being derived samples. In the context of a |
| + | pandemic other constraints are added because of social distancing measures.</p> |
| + | |
| + | <img |
| + | src="https://static.igem.org/mediawiki/2020/8/89/T--Paris_Bettencourt--Poster_SkinMicrobiomeStudy.png"> |
| + | |
| + | <p><b class="heading">2. Engineer the Skin Microbiome</b></p> |
| + | <ul> |
| + | <li><i>Staphylococcus epidermidis</i> is not used in synthetic biology and no tools are |
| + | currently available to engineer it</li> |
| + | <li>Thw two main challenges in using <i>S. epidermidis</i> in |
| + | synthetic biology are:</li> |
| + | </ul> |
| + | <img |
| + | src="https://static.igem.org/mediawiki/2020/1/1f/T--Paris_Bettencourt--Poster_SkinMicrobiomeEngineering.png"> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | |
| + | <!-- This creates a third width section with the id 'response' ---> |
| + | <!-- Response --> |
| + | <div class="section third" id="responses"> |
| + | <!--Here is the image used for the third width section, the size is: 400 x 200 pixels --> |
| + | <img src="https://static.igem.org/mediawiki/2020/5/5b/T--Paris_Bettencourt--Poster_Responses33.jpg"> |
| + | |
| + | <!--Info: Place the title and text for this section, it will appear when the user clicks on it--> |
| + | <div class="info"> |
| + | |
| + | <!--Write the title of the section --> |
| + | <div class="title"> Responses </div> |
| + | <!--Write the text explaining this section --> |
| + | <div class="text"> |
| + | <p><b class="heading">1. Quaranskin</b></p> |
| + | <p>Quaranskin is a study involving the collection of skin microbiome samples during the |
| + | COVID-19 pandemic and |
| + | observing the impact of environmental factors on the skin microbiome. |
| + | </p> |
| + | <ul> |
| + | <li>We took <b>ethical considerations</b> very seriously by submitting our project to |
| + | the French national ethics committee in order to obtain their approval before |
| + | starting the study |
| + | </li> |
| + | <li>In order to include <b>citizen participation</b>, we designed a smapling kit |
| + | containing all the material required for sampling the skin microbiome and set up |
| + | mailing system to ship and collect back the samples from participants |
| + | </li> |
| + | <li>We are building an <b>Open Database</b> of skin microbiome during the COVID-19 |
| + | pandemic |
| + | </li> |
| + | </ul> |
| + | <br /> |
| + | <p><b class="heading">2. EpiFlex, EpiGlow, EpiGrow</b></p> |
| + | <p>These three projects aims to develop tools to make S. epidermidis a good chassis for |
| + | Synthetic biology. |
| + | </p> |
| + | <ul> |
| + | <li><b>EpiFlex</b> is a MoClo tool kit for <i>S. epidermidis</i> </li> |
| + | <li><b>EpiGlow</b> is the proof of concept of EpiFlex that aims to optimize |
| + | transformation protocol <i>S. epidermidis</i></li> |
| + | <li><b>EpiGrow</b> is the optimization of <i>S. epidermidis</i> growth</i> </li> |
| + | </ul> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | |
| + | </div> |
| + | |
| + | |
| + | <!--This is the fourth row of the poster, it contains the Skin Microbiome section ---> |
| + | <!-- Skin Microbiome --> |
| + | <div class="row"> |
| + | <!-- This creates a full width section with the id 'skin_microbiome' ---> |
| + | <div class="section full" id="skin_microbiome"> |
| + | <img |
| + | src="https://static.igem.org/mediawiki/2020/a/a9/T--Paris_Bettencourt--Poster_TheSkinMicrobiome.jpg"> |
| + | |
| + | <!--Info: Place the title and text for this section, it will appear when the user clicks on it--> |
| + | <div class="info"> |
| + | <!--Write the title of the section --> |
| + | <div class="title">The Skin Microbiome</div> |
| + | <!--Write the text explaining this section --> |
| + | <div class="text"> |
| + | <p><b class="heading">What is the Skin Microbiome?</b></p> |
| + | <p>The human skin microbiome is a vast and very large ecosystem of microorganisms that |
| + | occupy the human skin at the level of the hypodermis, dermis, and epidermis. It is very |
| + | numerous and very diverse, a real petri dish hidden in plain sight. |
| + | </p> |
| + | <br /> |
| + | <p><b class="heading">How the Skin Microbiome plays a role in the skin health?</b></p> |
| + | <p>The human skin microbiome is very tightly linked to the health of its host in numerous |
| + | ways. First of all the microorganisms of the skin microbiome prevent the colonization of |
| + | the skin by pathological microorganisms, and help to train the immune system to make it |
| + | more prepared to face pathogens. It also directly involved in various pathologies, like |
| + | atopic dermatitis, where its overall diversity is reduced and Staphylococcus aureus is |
| + | over represented. |
| + | </p> |
| + | <br /> |
| + | <p><b class="heading">Why studying the composition of the Skin Microbiome?</b></p> |
| + | <p> |
| + | <ul> |
| + | <li>Most of the previous studies which lead to understand the skin microbiome by |
| + | sampling human volunteers were based on North American subjects. Form these results, |
| + | current evidence suggests a greater microbial diversity to be a beneficial trait, |
| + | however there is a need for greater diversity in the participant pools from which |
| + | this data is derived. Thus, a study observing human skin microbiome among a European |
| + | population would give new data increasing our knowledge of the skin microbiome. |
| + | </li> |
| + | <li>Besides the current global environment wherein a significant number of people are |
| + | minimizing their interactions with other people is a good opportunity to observe |
| + | environmental impact on skin microbiome in a more simplified model. |
| + | </li> |
| + | <li>Also, by taking samples from the skin microbiome during the COVID-19 pandemic, we |
| + | will have preserved evidence of the influence of the social restrictions induced by |
| + | the pandemic on the skin microbiome. |
| + | </li> |
| + | </ul> |
| + | </p> |
| + | <br /> |
| + | <img |
| + | src="https://static.igem.org/mediawiki/2020/8/80/T--Paris_Bettencourt--Poster_SkinMicrobiomeIllustration.png"> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | |
| + | <!--This is the fifth row of the poster, it contains the Quaranskin and Implementation section ---> |
| + | <!-- Quaranskin, Implementation --> |
| + | <div class="row"> |
| + | <!-- This creates a full width section with the id 'quaranskin' ---> |
| + | <!-- Quaranskin --> |
| + | <div class="section two_thirds" id="quaranskin"> |
| + | <img src="https://static.igem.org/mediawiki/2020/5/5b/T--Paris_Bettencourt--Poster_Quaranskin51.jpg"> |
| + | |
| + | <!--Info: Place the title and text for this section, it will appear when the user clicks on it--> |
| + | <div class="info"> |
| + | <!--Write the title of the section --> |
| + | <div class="title">Quaranskin</div> |
| + | <!--Write the text explaining this section --> |
| + | <div class="text"> |
| + | <p>Quaranskin, a combination of quarantine and skin, is a project based on the collection |
| + | and analysis of skin microbiome samples, collected from participants across Europe. |
| + | </p> |
| + | <p>We aim to understand if there are correlations between behavioral characteristics that |
| + | involve activity, hygiene and human interaction, and the diversity and composition of |
| + | skin microbes at four body sites. |
| + | </p> |
| + | <img |
| + | src="https://static.igem.org/mediawiki/2020/6/6e/T--Paris_Bettencourt--Poster_QuaranskinStudy1.png"> |
| + | <br /> |
| + | <p><b class="heading">Study Pipeline</b></p> |
| + | <br /> |
| + | <img |
| + | src="https://static.igem.org/mediawiki/2020/1/11/T--Paris_Bettencourt--Poster_QuaranskinStudy2.png"> |
| + | <p><b>Participants recruitment</b></p> |
| + | <p>Emails are sent to all the members of our institution, the CRI, and all the european |
| + | iGEMers, by focusing on people form countries where confinement or social distanciation |
| + | are set up. The total number of participants expected is 70. |
| + | </p> |
| + | <p><b>Participants enrollment</b></p> |
| + | <p>To be involved in the study each participants has to fill in a participation form and |
| + | link to it a signed and dated consent form. |
| + | </p> |
| + | <p>A link is sent to them allowing them to create a account on the OpenHumans platform in |
| + | order to furnish them an ID code which we use to keep anonymity |
| + | </p> |
| + | <p><b>Participants action</b></p> |
| + | <p>Once they are officially enroll in the study, we send to the participants by mail a kit |
| + | containing all tools needed to sample microbiome from 4 body sites and to send them back |
| + | to us, and by email a link to answer an online questionnaire asking questions covering 4 |
| + | main topics : The intrinsic characteristic (age, sexe, nationality...), the hygiene |
| + | habits, the level of confinement and the skin disorder appeared since February 2020. |
| + | </p> |
| + | <p><b>Microbiome sequencing</b></p> |
| + | <p>Once we receive the microbiome samples we send them directly to an external company, |
| + | Genewiz. They’ll extract bacterial DNA from the samples, amplify the V3-V4 regions of |
| + | the 16S RNA gene, and then sequence the amplicons. |
| + | </p> |
| + | <p><b>Statistical analysis</b></p> |
| + | <p>After having analyzed the composition and diversity of each microbiome we’ll link these |
| + | results to the answer to the questionnaire to finally find some correlations between |
| + | some microbiome compositions and some characteristics of the lifestyle or some skin |
| + | disorder |
| + | </p> |
| + | <br /> |
| + | |
| + | <p><b class="heading">Data Analysis</b></p> |
| + | <p><b>1. Diversity analysis by index of hygiene, personal information, level of restriction |
| + | </b></p> |
| + | <p>The microbiomes in the generated database are grouped by index value in each of the 3 |
| + | categories. By setting two indices, we can study the impact of the third element on the |
| + | diversity of the microbiome |
| + | </p> |
| + | <p><b>2. Environmental factors which influence proportion of Staphylococcus |
| + | </b></p> |
| + | <p>We define a proportion threshold, then we identify all the people who present a |
| + | population of Staphylococcus beyond this threshold. We finally look for parameters |
| + | common to these individuals, |
| + | </p> |
| + | <p><b>3. Researching existing microbiome composition among our data set |
| + | </b></p> |
| + | <p>We want to compare a typical composition of eczematic microbiome found in literature, |
| + | with our data |
| + | </p> |
| + | <p><b>4. Analysis of data based on common symptoms |
| + | </b></p> |
| + | <p>When a significant number of people present the same symptom, independently of the |
| + | environment and lifestyle, we want to make a synthesis of the typical composition of the |
| + | microbiome for this symptom. |
| + | </p> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | |
| + | |
| + | <!-- This creates a full width section with the id 'Implementation' ---> |
| + | <!-- Implementation --> |
| + | <div class="section third" id="Implementation"> |
| + | <img src="https://static.igem.org/mediawiki/2020/9/9b/T--Paris_Bettencourt--Poster_Implementation52.jpg"> |
| + | |
| + | <!--Info: Place the title and text for this section, it will appear when the user clicks on it--> |
| + | <div class="info"> |
| + | <!--Write the title of the section --> |
| + | <div class="title">Implementation</div> |
| + | <!--Write the text explaining this section --> |
| + | <div class="text"> |
| + | <p><b class="heading">Ethical consideration</b></p> |
| + | <p>As Quaranskin is a collection of data extracted from human derived samples (skin |
| + | microbiome) and personal information (answer to the questionnaire), it’s important to |
| + | learn about the ethical rules that govern research involving human being in Europe in |
| + | order to respect the protection of the participants in our study. |
| + | </p> |
| + | <p>In France, Research Involving Human Being (RIPH) has to be approved and framed by the |
| + | Committees for the Protection of Persons (CPP). |
| + | </p> |
| + | <p>We have submitted to the ethics committee a complete file presenting our study in detail. |
| + | This file consisted mainly of a research protocol and all the information documents for |
| + | the study participants. Even though we already recruit participant and set up all the |
| + | logistic aspect of the study, we are still waiting for their approval to collect and |
| + | analyse samples. |
| + | </p> |
| + | |
| + | <p><b class="heading">Contribution to the scientific community</b></p> |
| + | <ol> |
| + | <li>Increase the knowledge we have about the microbiome and more precisely about the |
| + | external factors that can impact it. |
| + | </li> |
| + | <li>Create an open database of skin microbiome from people in the context of a pandemic. |
| + | </li> |
| + | <li>Develop Science@Home by providing pipeline and protocols useful for future |
| + | microbiome studies based on citizen science |
| + | </li> |
| + | <li>Instruct participants about their microbiome by sending them back their microbiome |
| + | profile after analysis. |
| + | </li> |
| + | </ol> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | |
| + | <!--This is the sixth row of the poster, it contains the three nameless sections ---> |
| + | <!-- Engineer --> |
| + | <div class="row"> |
| + | <!-- This creates a full width section with the id 'engineer' ---> |
| + | <div class="section full" id="engineer"> |
| + | <!--Here is the image used for the full width section, the size is: 1200 x 200 pixels --> |
| + | <img src="https://static.igem.org/mediawiki/2020/4/40/T--Paris_Bettencourt--Poster_Engineering1.jpg"> |
| + | |
| + | <!--Info: Place the title and text for this section, it will appear when the user clicks on it--> |
| + | <div class="info"> |
| + | <!--Write the title of the section --> |
| + | <div class="title"> Engineering </div> |
| + | <!--Write the text explaining this section --> |
| + | <div class="text"> |
| + | <p>Biological engineering allowed us to develop solutions for our objectives.</p> |
| + | <p><b class="heading">Purpose</b></p> |
| + | <p>We aim to sense and modulate population dynamics of the skin microbiome in order to help |
| + | maintain its equilibrium and avoid dysbiosis induced pathologies. This is where we |
| + | envision the role of synthetic biology in the probiotic arena. |
| + | </p> |
| + | <p>More precisely, we thought about engineering <i>S. epidermidis</i> in order to control |
| + | overgrowth of <i>S. aureus</i> which can induce eczema. |
| + | </p> |
| + | <p><b class="heading"><i>S. epidermidis</i></b></p> |
| + | <p><i>S. epidermidis</i> represents approximately 90% of the aerobic skin flora, and about |
| + | 5% of |
| + | the overall skin microbiome species. It is a non-motile gram-positive coccus, and a |
| + | facultative anaerobe. Its mainly mutualistic behavior makes it a very good candidate to |
| + | become a vector for sensors or ways to modulate the skin microbiome. |
| + | </p> |
| + | <p><b class="heading">Research Focus</b></p> |
| + | <p>Three essential stages that we want to optimize to help engineer <i>S. epidermidis</i>: |
| + | </p> |
| + | <p></p> |
| + | <ol> |
| + | <li>Grow</li> |
| + | <li>Transform</li> |
| + | <li>Clone</li> |
| + | </ol> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | |
| + | |
| + | |
| + | <!--This is the seventh row of the poster, it contains the EpiFlex, EpiGlow and EpiGrow sections ---> |
| + | <!-- EpiFlex, EpiGlow, EpiGrow --> |
| + | <div class="row"> |
| + | <!-- This creates a third width section with the id 'epiflex' ---> |
| + | <div class="section third" id="epiflex"> |
| + | <!--Here is the image used for the third width section, the size is: 400 x 200 pixels --> |
| + | <img src="https://static.igem.org/mediawiki/2020/9/91/T--Paris_Bettencourt--Poster_EpiFlex71.jpg"> |
| + | <!--Info: Place the title and text for this section, it will appear when the user clicks on it--> |
| + | <div class="info"> |
| + | <!--Write the title of the section --> |
| + | <div class="title">EpiFlex</div> |
| + | <!--Write the text explaining this section --> |
| + | <div class="text"> |
| + | <div class="subsection half"> |
| + | <p>The bacterium Staphylococcus epidermidis has only few tools available for efficient |
| + | expression of recombinant DNA and genetic engineering. This generated the idea to |
| + | develop a MoClo toolkit for S.epidermidis, the EpiFlex toolkit. |
| + | </p> |
| + | <p>EpiFlex aims to make the bacterium a chassis for synthetic biology. |
| + | </p> |
| + | </div> |
| + | |
| + | <div class="subsection half logo-align"> |
| + | <img src="https://static.igem.org/mediawiki/2020/b/b0/T--Paris_Bettencourt--Poster_EpiFlexLogo.png" |
| + | class="logo"> |
| + | </div> |
| + | |
| + | <div class="clear"></div> |
| + | <br /> |
| + | |
| + | <p><b class="heading">MoClo Toolkit</b></p> |
| + | <p>The MoClo is a modular cloning method based on Golden Gate assembly. EpiFlex is a Moclo |
| + | toolkit developed with parts that function specifically in S.epidermidis. |
| + | </p> |
| + | <p><b class="heading">The MoClo Concept</b></p> |
| + | <img |
| + | src="https://static.igem.org/mediawiki/2020/6/6b/T--Paris_Bettencourt--Poster_MoCloConcept.png"> |
| + | |
| + | <p><b class="heading">Plasmid and Parts Design</b></p> |
| + | <img |
| + | src="https://static.igem.org/mediawiki/2020/9/9c/T--Paris_Bettencourt--Poster_PlasmidParts.png"> |
| + | <p>We chose a fluorescent reporter as a cloning selection marker to avoid the extra cost of |
| + | reagents needed in traditional screening methods such as blue-white screening which |
| + | requires X-gal. |
| + | </p> |
| + | <p>An important feature of our p1 and p2 backbones is the fact that they're <i>E. coli</i> -> <i>S. |
| + | epidermidis</i> shuttle vectors. This is important for a two step cloning workflow as <i>E. |
| + | coli</i> is an easy host for cloning and plasmid amplification before seeing the performance |
| + | of the cassette in <i>S. epidermidis</i>. |
| + | </p> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | |
| + | <!-- This creates a third width section with the id 'epiglow' ---> |
| + | <div class="section third" id="epiglow"> |
| + | <!--Here is the image used for the third width section, the size is: 400 x 200 pixels --> |
| + | <img src="https://static.igem.org/mediawiki/2020/9/9b/T--Paris_Bettencourt--Poster_EpiGlow72.jpg"> |
| + | |
| + | <!--Info: Place the title and text for this section, it will appear when the user clicks on it--> |
| + | <div class="info"> |
| + | <!--Write the title of the section --> |
| + | <div class="title">EpiGlow</div> |
| + | <!--Write the text explaining this section --> |
| + | <div class="text"> |
| + | <div class="subsection half"> |
| + | <p>EpiGlow is the proof of concept of our EpiFlex toolkit. We choose to express mCherry in S. epidermidis to demonstrate that our parts are functional. |
| + | </p> |
| + | </div> |
| + | |
| + | <div class="subsection half logo-align"> |
| + | <img src="https://static.igem.org/mediawiki/2020/9/9b/T--Paris_Bettencourt--Poster_EpiGlow72.jpg" |
| + | class="logo"> |
| + | </div> |
| + | |
| + | <div class="clear"></div> |
| + | <br /> |
| + | |
| + | <p>The expression of mCherry, a fluorescent protein, allows the easy characterization of different regulatory sequences, such as promoter, RBS and terminator. |
| + | </p> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | |
| + | <!-- This creates a third width section with the id 'epigrow' ---> |
| + | <div class="section third" id="epigrow"> |
| + | <!--Here is the image used for the third width section, the size is: 400 x 200 pixels --> |
| + | <img src="https://static.igem.org/mediawiki/2020/b/b0/T--Paris_Bettencourt--Poster_EpiGrow.jpg"> |
| + | |
| + | <!--Info: Place the title and text for this section, it will appear when the user clicks on it--> |
| + | <div class="info"> |
| + | <!--Write the title of the section --> |
| + | <div class="title"> Section 3 </div> |
| + | <!--Write the text explaining this section --> |
| + | <div class="text"> Use this section to explain whatever you would like! Suggestions: Safety, |
| + | Human Practices, Measurement, etc. </div> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | |
| + | |
| + | |
| + | |
| + | <!--This is the eighth row of the poster, it contains the future works sections ---> |
| + | <!-- Future Work --> |
| + | <div class="row"> |
| + | <!-- This creates a two thirds width section with the id 'future_work_1' ---> |
| + | <div class="section two_thirds" id="future_work_1"> |
| + | <!--Here is the image used for the third width section, the size is: 800 x 200 pixels --> |
| + | <img src="https://static.igem.org/mediawiki/2020/0/0b/T--Paris_Bettencourt--Poster_FutureWork81.jpg"> |
| + | |
| + | <!--Info: Place the title and text for this section, it will appear when the user clicks on it--> |
| + | <div class="info"> |
| + | <!--Write the title of the section --> |
| + | <div class="title"> Results</div> |
| + | <!--Write the text explaining this section --> |
| + | <div class="text"> What did your team achieve? What do you plan to work on moving forward? |
| + | </div> |
| + | </div> |
| + | |
| + | </div> |
| + | |
| + | <!-- This creates a third width section with the id 'future_work_2' ---> |
| + | <div class="section third" id="future_work_2"> |
| + | <!--Here is the image used for the third width section, the size is: 400 x 200 pixels --> |
| + | <img src="https://static.igem.org/mediawiki/2020/3/39/T--Paris_Bettencourt--Poster_FutureWork82.jpg"> |
| + | |
| + | <!--Info: Place the title and text for this section, it will appear when the user clicks on it--> |
| + | <div class="info"> |
| + | <!--Write the title of the section --> |
| + | <div class="title">References and Acknowledgements</div> |
| + | <!--Write the text explaining this section --> |
| + | <div class="text">If not already cited in other sections of your poster, what literature sources |
| + | did you reference on this poster? Who helped or advised you? </div> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | |
| + | |
| + | <!--This is the ninth row of the poster, it contains the iGEM experience section ---> |
| + | <!-- iGEM Experience--> |
| + | <div class="row"> |
| + | <!-- This creates a three quarters width section with the id 'iGEM' ---> |
| + | <div class="section full" id="iGEM"> |
| + | <img src="https://static.igem.org/mediawiki/2020/2/24/T--Paris_Bettencourt--Poster_iGEMExperience1.jpg"> |
| + | |
| + | <!--Info: Place the title and text for this section, it will appear when the user clicks on it--> |
| + | <div class="info"> |
| + | <!--Write the title of the section --> |
| + | <div class="title"> Our iGEM Experience</div> |
| + | <!--Write the text explaining this section --> |
| + | <div class="text"> |
| + | <!-- <img src="https://static.igem.org/mediawiki/2020/f/f8/T--Paris_Bettencourt--Poster_iGEMExperienceLogo.jpg" |
| + | class="logo"> |
| + | <div class="clear"></div> |
| + | <br /> --> |
| + | |
| + | <p>iGEM 2020 was a unique opportunity to meet other aspiring and motivated researchers that |
| + | helped broaden our knowledge on both human and scientific topics! We truly enjoyed our |
| + | experience while attending the virtual meetings and discussing on various topics with |
| + | iGEMers across the world. |
| + | </p> |
| + | <p><b class="heading">A Chit-Chat around the Skin Microbiome</b></p> |
| + | <p>In early June, our team enjoyed hosting a ChitChat session to discuss on the subject of |
| + | the Skin Microbiome. Several iGEM teams joined us in this session and provided us with |
| + | the opportunity in sharing our experience on this topic with them. |
| + | </p> |
| + | <p><b class="heading">iGEMeetParis: A Parisian Virtual Meetup</b></p> |
| + | <p>During the first weekend of September, we hosted our own virtual meetup, together with |
| + | the other Parisian teams. It took two months of preparation to set up an amazing weekend |
| + | lined up with workshops, hackathons, discussions, pitch presentations and other cool |
| + | social events! |
| + | </p> |
| + | <p><b class="heading">Cité des Sciences</b></p> |
| + | <p>We had the wonderful opportunity to display our project at the Cité des Sciences Annual |
| + | Science Fair in Paris where we shared our scientific communication with the general |
| + | public. |
| + | </p> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | |
| + | <!--This is the tenth row of the poster, it contains the acknowledgements section ---> |
| + | <!-- Acknowledgements --> |
| + | <div class="row"> |
| + | <!-- This creates a quarter width section with the id 'acknowlegments' ---> |
| + | <div class="section full" id="acknowlegments"> |
| + | <img src="https://static.igem.org/mediawiki/2020/4/42/T--Paris_Bettencourt--Poster_Acknowledgements.jpg"> |
| + | |
| + | <!--Info: Place the title and text for this section, it will appear when the user clicks on it--> |
| + | <div class="info"> |
| + | <!--Write the title of the section --> |
| + | <div class="title">Acknowledgements</div> |
| + | <!--Write the text explaining this section --> |
| + | <div class="text"> |
| + | <p><b class="heading">Acknowledgements</b></p> |
| + | <p>Valérie Antonio</p> |
| + | <p>Mad Price Ball</p> |
| + | <p>Céline Couteau</p> |
| + | <p>Paulina Ejsmont</p> |
| + | <p>Stéphanie Leclerc-Mercier</p> |
| + | <p>Valérie Lerouyer</p> |
| + | <p>Hamid Mebrouki</p> |
| + | <p>Piers Millett</p> |
| + | <p>Ian Monk</p> |
| + | <p>Julia Oh</p> |
| + | <p>Alexandre Singier</p> |
| + | <p>Jean-Christophe Thalabard</p> |
| + | <p>Bastian Greshake Tzovaras</p> |
| + | <br /> |
| + | |
| + | <p><b class="heading">Sponsors</b></p> |
| + | <div class="subsection third logo-align"> |
| + | <img src="https://static.igem.org/mediawiki/2020/9/95/T--Paris_Bettencourt--Poster_CRI.png" |
| + | class="logo"> |
| + | </div> |
| + | <div class="subsection third logo-align"> |
| + | <img src="https://static.igem.org/mediawiki/2020/a/a3/T--Paris_Bettencourt--Poster_FondationBettencourtSchueller.png" |
| + | class="logo"> |
| + | </div> |
| + | <div class="subsection third logo-align"> |
| + | <img src="https://static.igem.org/mediawiki/2020/4/47/T--Paris_Bettencourt--Poster_Geneious.png" |
| + | class="logo"> |
| + | </div> |
| + | <div class="clear"></div> |
| + | <br /> |
| + | |
| + | <div class="subsection third logo-align"> |
| + | <img src="https://static.igem.org/mediawiki/2020/e/e1/T--Paris_Bettencourt--Poster_Genewiz.png" |
| + | class="logo"> |
| + | </div> |
| + | <div class="subsection third logo-align"> |
| + | <img src="https://static.igem.org/mediawiki/2020/d/de/T--Paris_Bettencourt--Poster_IDT.png" |
| + | class="logo"> |
| + | </div> |
| + | <div class="subsection third logo-align"> |
| + | <img src="https://static.igem.org/mediawiki/2020/f/fc/T--Paris_Bettencourt--Poster_iGEM.png" |
| + | class="logo"> |
| + | </div> |
| + | <div class="clear"></div> |
| + | <br /> |
| + | |
| + | <div class="subsection third logo-align"> |
| + | <img src="https://static.igem.org/mediawiki/2020/6/65/T--Paris_Bettencourt--Poster_OpenHumans.png" |
| + | class="logo"> |
| + | </div> |
| + | <div class="subsection third logo-align"> |
| + | <img src="https://static.igem.org/mediawiki/2020/f/f6/T--Paris_Bettencourt--Poster_Snapgene.png" |
| + | class="logo"> |
| + | </div> |
| + | <div class="subsection third logo-align"> |
| + | <img src="https://static.igem.org/mediawiki/2020/8/8a/T--Paris_Bettencourt--Poster_TwistBioscience.png" |
| + | class="logo"> |
| + | </div> |
| + | <div class="clear"></div> |
| + | <br /> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | |
| + | |
| + | <!--This is where the selected content will be displayed - Don't change this section!--> |
| + | <div class="poster_w display"> |
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| + | <p id="section_text"></p> |
| + | </div> |
| + | |
| + | |
| + | </div> |
| + | </div> |