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Revision as of 22:17, 27 October 2020
Index:
Lab Notebook
Introduction
Below are our Benchling Protocol pages that show in detail the protocols we utilized in the construction of Manifold. For more details on how these protocols were utilized, see the Notebook page. For information on the planned use of these protocols, see the Experiments page.
Protocols
Agarose Gel Creation and PourAgarose Gel ElectrophoresisCulture from Agar Stab (Inoculation)DpnI IncubationGlycerol StockGolden Gate Assembly with BsaIGolden Gate Assembly with BsmBINEB Time-Saver qualified Restriction Enzyme DigestionNEBuilder Hifi MutagenesisPCR Purification Kit (GenScript)Phusion PCR ProtocolQIAGEN HiSpeed Plasmid PurificationQIAprep Spin Miniprep KitRestriction Enzyme(s) DigestSite Directed MutagenesisStandard Assembly (Column 3 - pduD + GFP)Standard Assembly (Combining Column 1 and 3)Transforming Competent CellsTRIzol DNA Extraction
