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| − | }</style><meta charset="utf-8"/><meta content="width=device-width,initial-scale=1" name="viewport"/><title>pFruB-Cra System | SugarGain | iGEM BITS Goa</title><link href="https://2020.igem.org/Template:BITSPilani-Goa_India/css/contentCSS?action=raw&ctype=text/css" rel="stylesheet"/></head><body><div id="safari-toast"><p>The Safari browser doesn't support some features of this website. 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| − | }</style><header class="d-flex justify-content-center"><div class="custom-container"><div id="header-background"></div><div id="heading"><h1>pFruB-Cra System</h1></div><a href="https://freepik.com" rel="noopener" target="_blank"><span data-placement="top" id="bg-attribution" title="Photo from Freepik"></span></a></div></header><main class="theme-journal"><div class="custom-container"><div class="row"><div class="content fullwidth"><article><div class="accordion" id="collapsibleSections"><div class="card"><div class="card-header" id="headingOne"><h2 class="mb-0"><button aria-controls="collapseOne" aria-expanded="true" class="btn collapsed" data-target="#collapseOne" data-toggle="collapse" type="button"><span>Invertase Inhibitor Assay</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingOne" class="collapse" id="collapseOne"><div class="card-body"><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/0/09/T--BITSPilani-Goa_India--docs--Experiments--pFruB-Cra_System--Anti-Invertase_Assay.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/0/09/T--BITSPilani-Goa_India--docs--Experiments--pFruB-Cra_System--Anti-Invertase_Assay.pdf" title="Invertase Inhibitor Assay" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingTwo"><h2 class="mb-0"><button aria-controls="collapseTwo" aria-expanded="true" class="btn collapsed" data-target="#collapseTwo" data-toggle="collapse" type="button"><span>pFruB Characterization</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingTwo" class="collapse" id="collapseTwo"><div class="card-body"><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/a/ae/T--BITSPilani-Goa_India--docs--Experiments--pFruB-Cra_System--pFruB_Characterisation.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/a/ae/T--BITSPilani-Goa_India--docs--Experiments--pFruB-Cra_System--pFruB_Characterisation.pdf" title="pFruB Characterization" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingThree"><h2 class="mb-0"><button aria-controls="collapseThree" aria-expanded="true" class="btn collapsed" data-target="#collapseThree" data-toggle="collapse" type="button"><span>pFruB Specificity</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingThree" class="collapse" id="collapseThree"><div class="card-body"><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/3/3c/T--BITSPilani-Goa_India--docs--Experiments--pFruB-Cra_System--pFruB_Specificity.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/3/3c/T--BITSPilani-Goa_India--docs--Experiments--pFruB-Cra_System--pFruB_Specificity.pdf" title="pFruB Specificity" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingFour"><h2 class="mb-0"><button aria-controls="collapseFour" aria-expanded="true" class="btn collapsed" data-target="#collapseFour" data-toggle="collapse" type="button"><span>Electrophoretic Mobility Shift Assay</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingFour" class="collapse" id="collapseFour"><div class="card-body"><p>To find the K<sub>d</sub> value of pFruB, we propose to use the following protocol.</p><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/4/4c/T--BITSPilani-Goa_India--docs--Experiments--Protocols--EMSA.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/4/4c/T--BITSPilani-Goa_India--docs--Experiments--Protocols--EMSA.pdf" title="Electrophoretic Mobility Shift Assay" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingFive"><h2 class="mb-0"><button aria-controls="collapseFive" aria-expanded="true" class="btn collapsed" data-target="#collapseFive" data-toggle="collapse" type="button"><span>Site Directed Mutagenesis</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingFive" class="collapse" id="collapseFive"><div class="card-body"><p>To tune the K<sub>d</sub> value of pFruB, we propose to use the following protocol.</p><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/a/ae/T--BITSPilani-Goa_India--docs--Experiments--Protocols--Site_Directed_Mutagenesis.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/a/ae/T--BITSPilani-Goa_India--docs--Experiments--Protocols--Site_Directed_Mutagenesis.pdf" title="Site Directed Mutagenesis" type="application/pdf"/></div></div></div></div></article><div id="buttons"><a class="button prev" href="https://2020.igem.org/Team:BITSPilani-Goa_India/Experiments"><span class="button-background"><span 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id="footerIcons"><a class="gmail" href="mailto:igembitsgoa@gmail.com"></a><a class="insta" href="https://instagram.com/igem_bits" rel="noopener" target="_blank"></a><a class="twitter" href="https://twitter.com/igembitsgoa" rel="noopener" target="_blank"></a></div></div></div></footer><script src="https://2020.igem.org/Template:BITSPilani-Goa_India/content-bundleJS?action=raw&ctype=text/javascript"></script></body></html> | + | }</style><header class="d-flex justify-content-center"><div class="custom-container"><div id="header-background"></div><div id="heading"><h1>pFruB-Cra System</h1></div><a href="https://freepik.com" rel="noopener" target="_blank"><span data-placement="top" id="bg-attribution" title="Photo from Freepik"></span></a></div></header><main class="theme-journal"><div class="custom-container"><div class="row"><div class="content fullwidth"><article><div class="accordion" id="collapsibleSections"><div class="card"><div class="card-header" id="headingOne"><h2 class="mb-0"><button aria-controls="collapseOne" aria-expanded="true" class="btn collapsed" data-target="#collapseOne" data-toggle="collapse" type="button"><span>Invertase Inhibitor Assay</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingOne" class="collapse" id="collapseOne"><div class="card-body"><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/0/09/T--BITSPilani-Goa_India--docs--Experiments--pFruB-Cra_System--Anti-Invertase_Assay.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/0/09/T--BITSPilani-Goa_India--docs--Experiments--pFruB-Cra_System--Anti-Invertase_Assay.pdf" title="Invertase Inhibitor Assay" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingTwo"><h2 class="mb-0"><button aria-controls="collapseTwo" aria-expanded="true" class="btn collapsed" data-target="#collapseTwo" data-toggle="collapse" type="button"><span>pFruB Characterization</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingTwo" class="collapse" id="collapseTwo"><div class="card-body"><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/a/ae/T--BITSPilani-Goa_India--docs--Experiments--pFruB-Cra_System--pFruB_Characterisation.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/a/ae/T--BITSPilani-Goa_India--docs--Experiments--pFruB-Cra_System--pFruB_Characterisation.pdf" title="pFruB Characterization" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingThree"><h2 class="mb-0"><button aria-controls="collapseThree" aria-expanded="true" class="btn collapsed" data-target="#collapseThree" data-toggle="collapse" type="button"><span>pFruB Specificity</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingThree" class="collapse" id="collapseThree"><div class="card-body"><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/3/3c/T--BITSPilani-Goa_India--docs--Experiments--pFruB-Cra_System--pFruB_Specificity.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/3/3c/T--BITSPilani-Goa_India--docs--Experiments--pFruB-Cra_System--pFruB_Specificity.pdf" title="pFruB Specificity" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingFour"><h2 class="mb-0"><button aria-controls="collapseFour" aria-expanded="true" class="btn collapsed" data-target="#collapseFour" data-toggle="collapse" type="button"><span>Electrophoretic Mobility Shift Assay</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingFour" class="collapse" id="collapseFour"><div class="card-body"><p>To find the K<sub>d</sub> value of pFruB, we propose to use the following protocol.</p><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/4/4c/T--BITSPilani-Goa_India--docs--Experiments--Protocols--EMSA.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/4/4c/T--BITSPilani-Goa_India--docs--Experiments--Protocols--EMSA.pdf" title="Electrophoretic Mobility Shift Assay" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingFive"><h2 class="mb-0"><button aria-controls="collapseFive" aria-expanded="true" class="btn collapsed" data-target="#collapseFive" data-toggle="collapse" type="button"><span>Site Directed Mutagenesis</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingFive" class="collapse" id="collapseFive"><div class="card-body"><p>To tune the K<sub>d</sub> value of pFruB, we propose to use the following protocol.</p><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/a/ae/T--BITSPilani-Goa_India--docs--Experiments--Protocols--Site_Directed_Mutagenesis.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/a/ae/T--BITSPilani-Goa_India--docs--Experiments--Protocols--Site_Directed_Mutagenesis.pdf" title="Site Directed Mutagenesis" type="application/pdf"/></div></div></div></div></article><div id="buttons"><a class="button prev" href="https://2020.igem.org/Team:BITSPilani-Goa_India/Experiments"><span class="button-background"><span aria-hidden="" class="circle"><span class="icon arrow"></span></span></span><span class="button-text">Experiments</span></a><a class="button next" href="https://2020.igem.org/Team:BITSPilani-Goa_India/Experiments/Kill_Switch"><span class="button-background"><span aria-hidden="" class="circle"><span class="icon arrow"></span></span></span><span class="button-text">Kill Switch</span></a></div></div></div></div></main><footer><div id="footerNav"><div 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To find the Kd value of pFruB, we propose to use the following protocol.
To download this document, click here.
To tune the Kd value of pFruB, we propose to use the following protocol.
To download this document, click here.