Team:IISER Berhampur/Model
Meeting with Prof Sudhir Krishna and Team
Intro :
Prof. Sudhir Krishna and his team members Dr. Meenakshi Iyer and Dr. Arun Sankaradoss from National Center For Biological Sciences, Bengaluru are working on the strains of Dengue that are endemic to India, in search of an effective vaccine. We reached out to them to understand the reason why we don't have a successful vaccine against Dengue.
They have worked before on NS1 and NS2 proteins, and were looking to extend their work on the other NS Proteins as well. They have sequenced genomes from four regions of India- Mumbai, Delhi, Jodhpur and Bangalore to get a wider understanding of the genome and its mutations in order to develop the vaccine. We discussed our project with them and we received insightful suggestions from them.
Insights :
1- After appreciating our approach towards looking for peptide inhibitors, they suggested that small molecule inhibitors could also be looked at.
2- They told us to look into the replication complex, as a potential target, with NS4 being the centre of it.
3- As for designing the peptides, they encouraged us to perform the MD simulations and even offered the prospect of a possible future collaboration in the MDS aspect.
4- In case we did not end up getting a good score from protein docking, they suggested we look into possible modifications like adding certain molecules and groups to increase the success rate and make the interactions more stable, by increasing the binding energy. Overall, it was a productive meeting that enabled us to understand the improvements that we could make to various aspects of our project.
Integration :
1- Our Dry lab members looked at the possibility of incorporating small molecule inhibitors and after reviewing we came to the conclusion that small molecules will have certain off target effects. Hence, it gave more clarity to our project and we clinged on it.
2- We incorporated MD simulations into our modelling .
1. Content
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1. This is different content
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1. This is ALSO different content
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