Team:Tsinghua/Parts

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Nitric oxide induced Biofilm Allayer

Basic Parts

Number Name Short Description Long Description
[1] BBa_K3369000 B.subtilis Nitric Oxide synthase n Bacillus Subtilis,nitric oxide is produced by Bacterial nitric-oxide synthases (bNOSs), which converts arginine into NO. In bacteria, since there is no corresponding reductive domain, additional reductive substances are needed for reduction. ALA is a heme precursor that acts as a corresponding reducing substrate.
[2] BBa_C0071 rhlR repressor/activator from P. aeruginosa PA3477 (+LVA) Transcriptional regulator, in complex with N-butyryl-HSL, RhlR binds to the Rhl promoter
[3] BBa_R0071 Promoter (RhlR & C4-HSL regulated) Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer. This is the natural sequence taken from Pseudomonas aeruginosa. Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.
[4] BBa_K1976048 Colicin E2 DNase domain "miniColicin" Colicin E2 is a peptide, that is toxic to coliform bacteria. It is one of a group of proteins of this type, it being a nicking endonuclease. It has a corresponding activity inhibiting protein called immunity protein Im2, which is also in the registry under the name BBa_K1976027. 

Composit Parts

Number Name Short Description Long Description
[1] BBa_K1893003 Rhl receiver with GFP reporter (RhlR+pRhl+GFP) This composite part is based on the Rhl quorum-sensing system from Pseudomonas aeruginosa. It consists of a Rhl receiver device and a GFP reporter that is activated in the presence of a C4-AHL signal.
[2] BBa_K3369002 B.subtilis Nitric Oxide synthase with C4-HSL report system B.subtilis Nitric Oxide synthase with C4-HSL report system

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