Our Contribution to the iGEM registry
In order to make a useful contribution for future iGEM teams we decided to add new information to existing parts in the registry. Since cell free systems are an attractive alternative for the production of proteins we aimed to analyse performance of consecutive promoters in such a cell free system.
For characterization, we picked 3 closely related promoters with a well documented difference in expression levels from the registry. In order to design the required plasmids we also chose 1 terminator from the registry. To accurately measure expression strength the fluorescent protein mCherry was utilized as the expressed protein. The complex of promotor, gene and terminator was assembled via Golden Gate cloning. Expression strengths were determined based on fluorescence measurements.
| Registry Number
| Part name
| Designed by
| Type
|
| BBa_J23101
| constitutive promoter family member
| Designed by: John Anderson
Group: iGEM2006_Berkeley (2006-08-04)
| Regulatory
|
| BBa_J23105
| constitutive promoter family member
| Designed by: John Anderson
Group: iGEM2006_Berkeley (2006-08-04)
| Regulatory
|
| BBa_J23109
| constitutive promoter family member
| Designed by: John Anderson
Group: iGEM2006_Berkeley (2006-08-04)
| Regulatory
|
| BBa_B1001
| Small, artificial terminator
| Designed by: Haiyao Huang Group: Knight Lab (2006-08-30)
| Terminator
|
| BBa_J06504
| mCherry, Red Fluorescent protein
| Designed by: ytwang Group: iGEM2005 (2005-07-18)
| Reporter mCherry
|
If your browser does not support embedded PDFs you can open the document seperately: Characterization - BOKU-Vienna - 2020
Our results can also be seen on the respective registry pages linked above as well as on our results page .
