Our project about light-induced lipid production yeast strain was partially inspired by the work of prof. Guo. The researchers have built the prototype to prove the concept (Fig 1). We decided to make an improved version that would be able to keep the environment at a constant temperature, control lighting conditions and would have a built-in steering device to provide equal lightning and supplementation throughout the cell culture.
Figure 1. Photograph of experimental setup (Guo et al., 2018)
We decided to make an improved version that would be able to keep the environment at a constant temperature, control lighting conditions and would have a built-in steering device to provide equal lightning and supplementation throughout the cell culture. After several design iterations, and the final version of the technical task was formulated:
Thus, at the end of August, our engineering team started to work on the device – "SANYA" (Simple Assembly of Nano Yeast autoproduction).
Work was split into two parts: mechanics and electronics. The first was to design a light- impenetrable case, a flask mount, and steering blades. On the electronics side, the key parts were:
The final list of used components was:
After a couple of sleepless nights, broken fingers, and burnt hands, our cutting edge masterpiece of engineering was finished and given to the lab for some 72-hour long stress-test from the very beginning.
SCHEMATIC GITHUB
Guo, J. et al. (2018) ‘Light-driven fine chemical production in yeast biohybrids’, Science. American Association for the Advancement of Science, 362(6416), pp. 813–816. doi: 10.1126/science.aat9777.
