Team:Exeter/Notebook/Oct21

Notebook (Exeter iGEM 2020)

Wednesday, 21 October

Chloe had a meeting with Velizar to discuss feedback on the Human Practices page, including feedback from John which we had received this morning. The main point was that the page needed to include more examples of what we had done rather than descriptions of the responsible innovation framework we used when researching Human Practices. Following this, Velizar, Izzy and Ariane got to work on rewriting the page.

Chloe had another meeting with all of us at about 6:30pm to plan out how we were going to follow up on wiki feedback and also what we were planning to put on the Registry.

Lab Work

During the meeting Chloe let us know some interesting news - that following our calcium carbonate precipitation experiment on Monday 12th of October, Mark had noticed crystals continuing to grow in some of the tubes, but only the nickel column flow-throughs and and the wash - in other words the solutions which did not contain carbonic anhydrase. This could either mean that our transformed E. coli expressed so much carbonic anhydrase that some of it came through in the wash, or that E. coli somehow has a way of sequestering CO2 and producing CaCO3 without carbonic anhydrase.

Hydrogels and Modelling

Velizar finalised the MATLAB program he and Mark had been working on on Monday. The graph produced showed a clear trend from yellow to red which confirmed that CO2 had successfully and effectively permeated our hydrogel:

graph showing change over time in ratio of yellow to red pixels from the photographs we took of our hydrogel experiment. CO2 permeated the hydrogel changing it from yellow to red due to a chemical reaction. The graph shows an upwards trend in the beginning followed by an unchanging high level of red pixels towards the end.

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