Team:Exeter/Notebook/Sept15

Notebook (Exeter iGEM 2020)

Tuesday, 15 September

Anna, Ariane, Bethan, Matthew and Pazzy went to Eloise's house to record voiceovers for the video or work on wiki pages.

Nina and Adam worked on gathering protocols for protein purification and enzyme kinetics experiments.

Lab Work

The camera recording yesterday's hydrogel experiment fell down in the middle of the night so today Izzy and Velizar went into the lab to get it set up again. However, even without the camera it was clear that the experiment had not worked because the hydrogel didn't change colour. The lab was full so they had to wait until tomorrow to redo the experiment.

Mark incubated the T7 constructs overnight. We will need to grow them tomorrow morning then induce them before extracting and purifying proteins using either nickel columns or protein purification tips.

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