Team:Jilin China/Implementation

Overview
Instruction
Rules
Principles
Safety
Chanllenge

Overview

Our game is open to anyone interested in biology games, but children under 14 should be accompanied by a guardian for the experience. 

The basic structure of our product is a two-layer box and a culture medium containing E. coli inside. The outer box is opaque and removable, which means that the player can change the inner box, which is made of transparent plexiglas and contains either solid medium or liquid medium, from which the player can choose. The outer box containing solid medium is facilited with a camera inside the bottom, while the outer box containing liquid medium is equipped with a small spectrophotometer. On the top of the inner box are two-tone LED lights and a small cartridge containing 8mL of 75% ethanol. The wavelength and intensity of the light can be adjusted by means of a knob on the outer box, and pressing a button on the outer box controls the cartridge to create an alcohol spray.

Instruction

During the game, by turning the knob on the outer box, players can choose and vary the wavelength (from 230 to 700nm) and intensity (from 0 to 600 μW/cm2) of light to determine the state and strength of the player's bacteria defense and counterattack. At wavelengths near 460 nm, blue light turns on expression of the antitoxin RelB, lifting growth inhibition; at wavelengths near 535 nm, green light turns on counterattack against player bacteria, and dark or far infrared turns off counterattack.

Rules

For solid medium, the territory of enemy bacteria and player bacteria can be directly observed through camera imaging, and the area occupied by each on the medium can be counted for scoring and judging the player's victory; for liquid medium, the fluorescence intensity can be measured for scoring and judging the player's victory.

Principles

Player (with color) 

Attacked and defense: They are induced to express the endotoxin RelE by arabinose thus their growth is inhibited, and can be acivated by blue light to express the antitoxin RelB to undo the growth inhibition.

Acivated by green light to express AHL,which induces the foe bacteria to express the endotoxin RelE and their growth is inhibited.

 Enemy (without color)

Attack: Constructively express xylanase thus hydolyze xylan, and the product arabinose induces the player bacterium to commit inhibition by expressing the endotoxin RelE.   

Attacked: They can be induced to express the endotoxin RelE and their growth is inhibited.

Safety

The culture medium is sealed in a double-layer box, so the player will not have direct contact with the bacteria. When the game is over, the alcohol spray on the inner box can be turned on via a button on the outer box to kill the E. coli bacteria in the culture medium. When purchasing the product, the player is required to sign an agreement that they will not actively destroy the box causing environmental pollution or health hazards,and recycle them according to bio-waste.

Chanllenge

We are not sure that the players can fully understand the principles of this game. More importantly, the biosafety concerning it is still to be further ensured, including the potential  environmental pollution and health hazards.