Team:NCKU Tainan/Parts
Overview
BioBricks have always been the foundational building blocks for iGEM projects. When constructing our BioBricks, we made use of the IDT gBlock synthesis offer and Twist gene synthesis to generate our required parts in a fast and efficient way. Additionally, we made use of PCR amplification with our constructed expression plasmids as a template to construct several BioBricks. All of the parts contain a coding sequence, some of which extended with promoters or introns. We created a part collection of basic parts and improved composite parts. All parts were generated during our project.
Basic parts
| Name | Description | Type | Length |
|---|---|---|---|
| BBa_B0015 | Double terminator (B0010-B0012) | Terminator | 129bp |
| BBa_B0034 | Ribosome binding site | RBS | 12 bp |
| BBa_C0012 | LacI repressor (LacI) | Repressor | 25 bp |
| BBa_C0040 | Coding region for the TetR protein without the RBS (TetR) | Coding | 685 bp |
| BBa_C0080 | Arac arabinose regulatory protein (Arac) | Coding | 940 bp |
| BBa_E0040 | Green fluorescent protein (GFP) | Coding | 720 bp |
| BBa_I13453 | pBAD promoter associates with araC (pBAD) | Promoter | 130 bp |
| BBa_J23100 | Constitutive promoter (J23100) | Promoter | 35 bp |
| BBa_J61020 | Site for recombination by flp recombinase (FRT) | DNA sites | 34 bp |
| BBa_J64997 | T7 promoter | Promoter | 19 bp |
| BBa_R0051 | Promoter regulated by CI857 protein (pR) | Promoter | 49 bp |
| BBa_K079036 | Wild Type operator sequence regulating Tetracycline resistance genes. | Coding | 15 bp |
| BBa_K1015001 | Site specific recombinase (FLP) | Coding | 1272 bp |
| BBa_K105001 | VP16-eukaryotic activation domain | Coding | 276 bp |
| BBa_K117000 | Lysis gene(Lysis) | Coding | 144 bp |
| BBa_K1404006 | Allow E. coli to form biofilms (csgA) | Coding | 453 bp |
| BBa_K1429003 | Orange fluorescent protein (OFP) | Coding | 703 bp |
| BBa_K200011 | Heat-sensitive protein (CI857) | Repressor | 869 bp |
| BBa_K2332002 | Blue light inducible promoter associates with EL222 (pBlind) | Promoter | 53 bp |
| BBa_K2332004 | Blue light-sensitive protein (EL222) | Coding | 675 bp |
| BBa_K3198000 | Degrade mRNA to hibernate E. coli (hicA) | Coding | 177 bp |
| BBa_K3198001 | Neutralize the effect of hicA (hicB) | Coding | 417 bp |
| BBa_K3490000 | Nitric oxide synthase [Bacillus subtilis] | Coding | 1095 bp |
| BBa_K3490888 | LacO | DNA | 25 bp |
| BBa_K3490999 | pLac, core promoter (without CAP region and Lac operator) | Promoter | 31 bp |
| BBa_K611049 | Tetracycline repressible promoter (pTET) | Promoter | 54 bp |
| BBa_K805015 | Allowing E. coli to form biofilms (csgD) | Coding | 648 bp |
Composite parts
| Name | Description | Type | Length |
|---|---|---|---|
| BBa_K3490001 | pLac-csgA-csgD-terminator- T7 promoter-bsNOS-LacI | Composite | 4001 bp |
| BBa_K3490002 | BBa_K3490999-RBS-csgA-csgD-B0015 | Composite | 1400 bp |
| BBa_K3490003 | BBa_J64997-BBa_K3490888-RBS-BBa_K3490000 | Composite | 1173 bp |
| BBa_K3490011 | J23100-TetR-VP16-TetO-J23100-Lysis | Composite | 3543 bp |
| BBa_K3490020 | J23100-EL222-pBlind-FLP-terminator-hicB-FRT-pBAD-FRT-hicA | Composite | 5417 bp |
| BBa_K3490021 | J23100-EL222-pBlind-FLP-terminator-sfGFP-FRT-J23100-FRT-OFP | Composite | 3920 bp |
| BBa_K3490030 | araC-FRT-pBAD-hicA-FRT | Composite | 1533 bp |
| BBa_K3490031 | J23100-hicB | Composite | 583 bp |
