Team:NWU-CHINA-A/Safety

Overview

NWU-CHINA-A team understands the inherent risks of working in the laboratory and is committed to taking all necessary precautions to ensure that no personal or environmental harm is caused. To this end, we have implemented the following security procedures below. Our complete security form can be found here.

General Laboratary Safety

Project Specific Laboratory Safety

Our project this year involves the basic genetic operation of E.coli DH5α and BL21. According to German Collection of Microorganisms and Cell Cultures GmbH, it is defined as the Risk 1 Group with low potential risk. In our wet lab, all the upstream genetic engineering operations, such as transformation, vector expression and protein measurement, are strictly obeyed the regulations and supervised by teachers or postgraduate.

GeneGreen nucleic acid dye is an oily macromolecule modified based on cyanine.The dye can not penetrate the cell membrane and enter the living cells. It is not easy to volatilize and be inhaled into the human body. The mutagenicity of the dye is much lower than that of EB.

Considering the risk of bacterial escape, all our engineered bacteria are killed by 75% alcohol.

GeneGreen nucleic acid dye

Training & Enforcenment

Training: Before starting work in the laboratory, all team members working in the laboratory must complete the complete training of molecular biology experiments under the guidance of laboratory staff and teachers. These courses have set up specific guidelines and are standard requirements for work in the first class laboratories in Shaanxi, China.

Enforcement: Team members who violate safety rules must work under the supervision of the lab staff until the lab staff believes that the team members are capable of performing tasks without supervision. In cases of repeated violations or complete non-compliance with safety regulations, members are restricted to laboratory work until they are retrained and show full compliance with safety regulations to the test staff.