Team:Purdue/Contribution
This year Purdue iGEM designed a 3D printable microfluidic chip that is fully available to other teams for use in future projects. The chip is designed for high throughput RNA extraction and sequence detection and includes space for an experimental control design. After consulting several academic experts, which is detailed on our Human Practices page, we developed the following final model shown in the video below:
There are a total of 4 inputs on the chip: input 1 labelled in red (see figure1) for the diluted saliva sample and inputs 4,5, and 6 labelled in different shades of yellow (see figure1) for adding reagants into the reaction chamber for RPA and Argonaute detection. RNA is extracted from saliva samples using a chitosan membrane that is to be embedded between the top and middle layers (see figure 2) between inlet 1 and outlet 2 (see figure1) as indicated by the green band in the diagram below. A waste outlet for removing the reagents from the chitosan washes is indicated in gray (see figure1). There is also an outlet hole located above the RPA chamber for pressure control (see figure1). All outlets are 1.5mm in diameter which is the standard syringe pump inlet size. The current design consists of four printable layers (see figure1). As we continue to develop this project and move into phase II, we hope to improve on this design and increase the modularity of this device. The CAD models of the microfluidic chip were developed for 3D printing and laser cutting. The chip layers can be assembled via 3D printing by applying an industrial adhesive between the layers. The CAD models can also be used to laser cut PDMS and bond the layers along with other electronic components via standard lithography. Link to folder containing the CAD files of the microfluidic chipContribution
Final Microfluidic Chip Design
CAD Files