Team:St Andrews/Contribution

Shinescreen: A Novel,Entirely Reef-Safe Probiotic Sunscreen.


All parts non-native to E. coli were codon optimised using the IDT codon optimisation tool ( Codon optimisation ensures that the codons within our parts correspond to the codon bias of our chassis. The pre-existing biobricks for the parts used are listed below, as well as the codon optimised replacement created by our team.

Part Name Pre-existing BioBrick Codon Optimised Replacement
3-Dehydroquinate Synthase (DHQS) BBa_K814000 BBa_K3634000
O-methyltransferase (O-MT) BBa_K814002 BBa_K3634001
ATP-Grasp (ATPG) BBa_K814001 BBa_K3634002
Nonribosomal Peptide Synthetase (NRPS) BBa_K814003 BBa_K3634003
ccaS BBa_K592001 BBa_K3634006
ccaR BBa_K592002 BBa_K3634017
Heme Oxygenase (ho1) BBa_K2328062 BBa_K3634007
Phycocyanobilin:Ferredoxin Oxidoreductase (pcyA) BBa_I15009 BBa_K3634008

For some of these parts, in silico site-directed mutagenesis was carried out as to remove illegal BioBrick standard restriction sites introduced by the IDT codon optimisation tool.

Alongside creating new codon optimised parts, we also added additional information from the literature to the following parts:

* the UirS/UirR system formed part of our early gene circuit design but was omitted from our final design.

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