Team:TU Darmstadt/Project/Backbones
For the in silico design of our experiments for the wetlab we used several different plasmid backbones. Due to the COVID-19 pandemic we were not able to test any of our constructs in the lab. Therefore, we only planned the shown backbones theoretically. The plasmids would be used for cloning, expression, and genomic integration of our constructs.
Backbone Table
| Name | Size [bp] | Resistance | Features | Organism |
|---|---|---|---|---|
| pET24(+) | 5236 | Kanamycin | KanR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, f1 ori, lacI repressor + lacI promoter, T7 promoter + lac operator, 6xHis, T7 terminator | E. coli |
| pSEVA3b67Rb | 4617 | Chloramphenicol | high-copy-number pUC ori, pTHT15 ori, repB, mRFP1, rrnB T1 terminator, oriT, lambda t0 terminator | E. coli, B. subtilis |
| pJET1.2 | 2974 | Ampicillin | AmpR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori | E. coli |