Team:UNSW Australia/Notebook

WEEK 1

31st August - 6th September

Cloning

WEEK 2

7th September - 13th September

Protein Expression and Purification

WEEK 3

14th September - 20th September

Protein Expression and Purification

  • Cell lysis of harvested cells by sonication
  • Protein purification attempt #1 using gravity column.
  • SDS-PAGE of purification attempt #1.
  • Protein purification attempt #2 using centrifugation alongside gravity column.
  • SDS-PAGE of purification attempt #2.

WEEK 4

21st September - 27th September

Protein Expression and Purification

WEEK 5

28th September - 4th October

Characterisation and Assay

  • Prepared Dialysis Buffer.
  • Dialysis attempt #1.
  • Prepared new Dialysis Buffer.
  • Dialysis attempt #2.

WEEK 6

5th October - 11th October

Characterisation and Assay

  • Research and design process to refine Bradford assay protocol.
  • Protein concentration assay (Bradford Assay).
  • Research and design process to create citrate synthase assay protocol.

WEEK 7

12th October - 18th October

Characterisation and Assay

  • Citrate synthase assay attempt #1.
  • Research and design process to refine citrate synthase assay protocol.
  • Citrate synthase attempt #2.

WEEK 8

19th October - 23rd October

Characterisation and Assay