Team:USAFA/Parts
Part Table
Improvement of Existing Part
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Parts
| Part | Usage | Purpose | Creation |
|---|---|---|---|
| Improved prmA from Rhodococcus jostii | PFAS Biosensor | PFAS sensitive promoter | Sequenced from RHA1 |
| Codon Optimized mRFP for Rhodococcus jostii RHA1 | PFAS biosensor | Reporter for RHA1 | Modification of Biobrick mRFP |
| Delftia acidovorans haloacid dehalogenase 1, codon optimized for E.coli | PFOA/S destruction | Defluorinate PFOA/S | Codon optimized gene from Delftia |
| Delftia acidovorans haloacid dehalogenase 2, codon optimized for E. coli | PFOA/S destruction | Defluorinate PFOA/S | Codon optimized gene from Delftia |
| DeHa1.2.0 | PFOA/S destruction | Express native Dehalogenase 1 | DeHa1 in a T7 expression block with flanking Biobrick prefix and suffix as well as VF2/VR sites |
| DeHa1.2.HisN | PFOA/S Destruction | Express Dehalogenase 1 with N terminal His tag | DeHa1 in a T7 expression block with flanking Biobrick prefix and suffix as well as VF2/VR sites |
| DeHA1.2.HisCl | PFOA/S destruction | Express Dehalogenase 1 with C terminal His tag | DeHa1 in a T& expression block with flanking Biobrick prefix and suffix as well as VF2/VR sites |
| DeHa2.2.0 | PFOA/S destruction | Express native Dehalogenase 2 | DeHa2.2 in a T7 expression block with flanking Biobrick prefix and suffix as well as VF2/VF sites |
| DeHa2.2.HisN | PFOA/S destruction | Express Dehalogenase 2 with N terminal His tag | DeHa1 in a T7 expression block with flanking Biobrick prefix as well as VF2/VR sites |
| DeHa2.2.HisC | PFOA/S destruction | Express Dehalogenase 2 with C terminal His tag | DeHa1 in a T7 expression block with flanking Biobrick prefix and suffix as well as VF2/VR sites |
