Safety
Safety is always the most primary issue to consider, especially for projects of which potential consumers are the vulnerable elderly. The risks for everyone who has access to engineered probiotic from production to consumption should be carefully identified and managed.
Laboratory Safety
The Fudan team is fully compliant with the safety and security policies of the iGEM. Safety has been carefully and thoroughly considered throughout our project design and experimental operation.
Safety training
All members in our team have taken online courses in biosafety, such as hazardous chemicals, laboratory waste, special equipment safety, accident handling, and passed laboratory safety examination required by Assets and Security Department of our university before we entering the lab.
Lab work
Our experiments were all performed in the laboratory of Prof. Liang Cai in School of Life Sciences, which is qualified for Biosafety Level 2. For safety equipment, the lab has biosafety cabinets allowing us to carry out most experiments safely. There is a cabinet to store the hazardous reagents, the key of which only Prof. Cai has and have a dedicated refrigerator for iGEM to store our experimental materials including plasmids and primers. Fire extinguishers, fire hydrants, emergency spray and other safety facilities are also available in the lab to deal with different accidental situations. As the supervisor of lab, Prof. Cai also provided us with systematic safety guidelines and in field training before we started lab affair. Every time we conducted our experiments, at least one senior student was present overseeing our daily experimental operations following all the guidelines. Some of the guidelines are as follows:
However, for the Chinese population, the bottlenecks are as follows:
- The experimental bench and the rest of area should be strictly separated. Everyone performing experiments must wear Lab -gown and nitrile gloves before getting into the experimental area. Masks should also be wearing to prevent E. coli from being taken in. Lab -gown, gloves and any other used personal protection equipment must not be taken out of the experimental area.
- Experiments using hazardous chemicals like Ethidium Bromide should be conducted on special bench. While conducting, experimenters must wear an additional pair of nitrile gloves to protect themselves and avoid contaminating the inner gloves.
- If wanting to use the high-handed sterilization pan, students should ask supervisor or other senior students to operate instead.
- The last person to leave the lab should make sure that water, electricity, and the air conditioner are shut down, and lock all the doors and windows before leaving.
Avoid contamination
All kinds of wastes were disposed following the laboratory waste disposal policy strictly. Normal laboratory wastes are collected and emptied regularly. Contaminative and toxic waste was separately stored according to their chemical properties waiting for specialists to treat. All containers in contact with bacteria were sterilized before and after use. Experiments that might cause bacteria contamination are performed in Biosafety cabinets. Our culture medium and plates were sealed and preserved in separate refrigerators. In addition, our genetic materials are provided directly from Prof. Cai’s lab or ordered from local biotechnology company, so there were no transfer or shipment problem involved. We have always transferred our information and experimental data with great deliberation.
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