Difference between revisions of "Team:TU Darmstadt/Project/Backbones"

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       <th scope="col">Resistance</th>
 
       <th scope="col">Resistance</th>
 
       <th scope="col">Features</th>
 
       <th scope="col">Features</th>
 +
      <th scope="col">Organism</th>
 
     </tr>
 
     </tr>
 
   </thead>
 
   </thead>
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       <td>Kan</td>
 
       <td>Kan</td>
 
       <td>KanR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, f1 ori, lacI repressor + lacI promoter, T7 promoter + lac operator, 6xHis, T7 terminator</td>
 
       <td>KanR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, f1 ori, lacI repressor + lacI promoter, T7 promoter + lac operator, 6xHis, T7 terminator</td>
 +
<td><i>E. coli</i></td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td scope="row">2</th>
+
       <td scope="row">Shuttle vector</th>
−
       <td>Jacob</td>
+
       <td>Rom-Size</td>
−
       <td>Thornton</td>
+
       <td>Kanamycin</td>
−
       <td>@fat</td>
+
       <td>features</td>
 +
      <td><i>E. coli</i>, <i>B. subtilis</i></td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td scope="row">3</th>
+
       <td scope="row">Kill switch vector?</th>
−
       <td colspan="2">Larry the Bird</td>
+
       <td>Rom-Size</td>
−
       <td>@twitter</td>
+
       <td>Antibiotic resistance</td>
 +
  <td>features</td>
 +
  <td><i>E. coli</i>, <i>B. subtilis</i></td>
 
     </tr>
 
     </tr>
 
   </tbody>
 
   </tbody>

Revision as of 12:48, 15 October 2020

image/svg+xml - O O

Backbones

Backbones Table
University Experts Former iGEMers Others

Backbones

For the in silico design of our experiments for the wetlab we used several different plasmid backbones. Due to Covid-19 we were not able to test any of our constructs in the lab. Therefore, we only planned the shown backbones theoretically. The plasmids would be used for cloning, expression, and genomic integration of our constructs. You can find a list of all plasmids we used for our design and the corresponding parts below.
Name Size Resistance Features Organism
pET24(+) 5236bp Kan KanR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, f1 ori, lacI repressor + lacI promoter, T7 promoter + lac operator, 6xHis, T7 terminator E. coli
Shuttle vector Rom-Size Kanamycin features E. coli, B. subtilis
Kill switch vector? Rom-Size Antibiotic resistance features E. coli, B. subtilis