Difference between revisions of "Team:TU Darmstadt/Parts"
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<div class="col-sm-12 col-xl-10"> | <div class="col-sm-12 col-xl-10"> | ||
| − | + | <br> | |
| + | <div class="TFcontainer"> | ||
| + | <div class="containertext" id=Chapter1 > | ||
| + | <b>Due to Covid-19 </b> we were <b> not able to test </b> any of our designed parts in the lab. Nevertheless, we registered our basic parts, so <b> future teams </b> are easily able to characterize them. For our <b> Composite Parts we refrained from uploading </b> them to the registry as there exists no primary literature on them, but we still provide schematic images on them. | ||
| + | </div> | ||
| + | </div> | ||
<h1><b> Basic Parts </b></h1> | <h1><b> Basic Parts </b></h1> | ||
<div class="TFcontainer"> | <div class="TFcontainer"> | ||
<div class="containertext" id=Chapter1 > | <div class="containertext" id=Chapter1 > | ||
| − | + | <b>Due to Covid-19 </b> we were <b> not able to test </b> any of our designed parts in the lab. Nevertheless, we registered our basic parts, so <b> future teams </b> are easily able to characterize them. For our <b> Composite Parts we refrained from uploading </b> them to the registry as there exists no primary literature on them, but we still provide schematic images on them. | |
| − | + | ||
| − | + | All our <b> basic parts </b> are depicted in the table below. Because we were <b> not able to conduct any lab work </b>, we did not submit any parts for the special prizes Best Basic Part. | |
| − | + | ||
| − | + | ||
</div> | </div> | ||
</div> | </div> | ||
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<h1><b> Composite Parts </b></h1> | <h1><b> Composite Parts </b></h1> | ||
<h1> For <i>in vitro</i> characterization </h1> | <h1> For <i>in vitro</i> characterization </h1> | ||
| + | <div class="TFcontainer"> | ||
| + | <div class="containertext" id=Chapter1 > | ||
| + | For purification and <I> in vitro </I> characterization of our enzymes we <b> designed the following Composite Parts </b>: <i>cotA</i>, <i> cueO</i>, and <i> ereB</i>. All of these Composite Parts contain a strep-tag for purification and are optimized for <I> E. coli</I>. | ||
| + | </div> | ||
| + | </div> | ||
<figure> | <figure> | ||
<img src="https://static.igem.org/mediawiki/2020/5/58/T--TU_Darmstadt--Composite_Pharmies.png" alt="figure" > | <img src="https://static.igem.org/mediawiki/2020/5/58/T--TU_Darmstadt--Composite_Pharmies.png" alt="figure" > | ||
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<h1> For Immobilization on our biofilm </h1> | <h1> For Immobilization on our biofilm </h1> | ||
| + | <div class="TFcontainer"> | ||
| + | <div class="containertext" id=Chapter1 > | ||
| + | The following parts were designed for immobilization of our enzymes on the TasA matrix protein of our <I>B. subtilis </I> biofilm. In these composite parts the <b> enzymes are genetically fused to TasA </b>. | ||
| + | </div> | ||
| + | </div> | ||
<div class="containerimg" id=Chapter2> | <div class="containerimg" id=Chapter2> | ||
<figure> | <figure> | ||
Revision as of 15:03, 17 October 2020
Due to Covid-19 we were not able to test any of our designed parts in the lab. Nevertheless, we registered our basic parts, so future teams are easily able to characterize them. For our Composite Parts we refrained from uploading them to the registry as there exists no primary literature on them, but we still provide schematic images on them.
Basic Parts
Due to Covid-19 we were not able to test any of our designed parts in the lab. Nevertheless, we registered our basic parts, so future teams are easily able to characterize them. For our Composite Parts we refrained from uploading them to the registry as there exists no primary literature on them, but we still provide schematic images on them.
All our basic parts are depicted in the table below. Because we were not able to conduct any lab work , we did not submit any parts for the special prizes Best Basic Part.
| Part(BBa_) | Type | Description | Length in bp |
|---|---|---|---|
| BBa_K3429000 | Coding Sequence | Otto | @mdo |
| BBa_K3429001 | Coding Sequence | Otto | @mdo |
| BBa_K3429002 | Coding Sequence | Otto | @mdo |
| BBa_K3429003 | Coding Sequence | Otto | @mdo |
| BBa_K3429004 | Coding Sequence | Otto | @mdo |
| BBa_K3429005 | Coding Sequence | Otto | @mdo |
| BBa_K3429006 | Coding Sequence | Otto | @mdo |
| BBa_K3429007 | Coding Sequence | Otto | @mdo |
| BBa_K3429008 | Coding Sequence | Otto | @mdo |
| BBa_K3429009 | Coding Sequence | Otto | @mdo |
| BBa_K3429011 | Coding Sequence | Otto | @mdo |
| BBa_K3429012 | Coding Sequence | Otto | @mdo |
| This could be your part number | Coding Sequence | Otto | @mdo |
Composite Parts
For in vitro characterization
For purification and in vitro characterization of our enzymes we designed the following Composite Parts : cotA, cueO, and ereB. All of these Composite Parts contain a strep-tag for purification and are optimized for E. coli.
For Immobilization on our biofilm
The following parts were designed for immobilization of our enzymes on the TasA matrix protein of our B. subtilis biofilm. In these composite parts the enzymes are genetically fused to TasA .
