页面摘要，或者是单项奖核心点 Our project aims at converting engineered Our project aims at converting engineered Our project aims at converting engineered Our project aims at converting engi neered Our project aims at converting engineered Our project aims at converting engineered Our project aims at converting engineered Our project aims at converting engineered

Cryopreservation is a technique that store cells at a very low temperatures (-80℃) to reduce cell metabolic damage and enable long-term storage.

1. Add cryoprotectant: Add 80% glycerin 500μL and bacterial fluid 1 000μL into Cryopreservation vials. Mix upside down gently.
2. Seal the vials: Stick sellotape around the label.
3. Store the vials: Store prepared vials in refrigerator at -80℃.

Bacteria preserved at -80 ℃ need to be recovery to restores cell growth.

1. Prepare plate: Take out the plate with relevant resistance from 4 degrees and wait for the temperature to the room temperature. Mark at the bottom of the plate.
2. Recover the cell: The -80 ° C frozen strain was removed and placed on the ice quickly. Take a ring of the upper layer of melt bacteria liquid by the sterilized inoculation ring. Coated the plate and draw a line.
3. Culture the recovered cell: Incubate at 37 degrees overnight./li>

PCR is performed for amplify DNA fragments in our project.

1. Set up PCR system (25µl):

   Reagent	Volume
   ddH2O	To 25 µl
   2 x Phanta Max Buffer	12.5 µl
   dNTP	0.5 µl
   Phanta	0.5 µl
   Template	Plasmid: 0.5 µl PCR product: 1µl
   Forward Primer	1 µl
   Reverse Primer	1 µl

2. Seal the vials: Stick sellotape around the label.
3. The expression level of short peptides has a threshold, preventing any excessive calcium absorption that might lead to hypercalcemia 注意句子超长后的回折
4. Store the vials: Store prepared vials in refrigerator at -80℃.

Cryopreservation is a technique that store cells at a very low temperatures (-80℃) to reduce cell metabolic damage and enable long-term storage.

1. Add cryoprotectant: Add 80% glycerin 500μL and bacterial fluid 1 000μL into Cryopreservation vials. Mix upside down gently.
2. Seal the vials: Stick sellotape around the label.
3. The expression level of short peptides has a threshold, preventing any excessive calcium absorption that might lead to hypercalcemia 注意句子超长后的回折
4. Store the vials: Store prepared vials in refrigerator at -80℃.

Cryopreservation is a technique that store cells at a very low temperatures (-80℃) to reduce cell metabolic damage and enable long-term storage.

1. Add cryoprotectant: Add 80% glycerin 500μL and bacterial fluid 1 000μL into Cryopreservation vials. Mix upside down gently.
2. Seal the vials: Stick sellotape around the label.
3. The expression level of short peptides has a threshold, preventing any excessive calcium absorption that might lead to hypercalcemia 注意句子超长后的回折
4. Store the vials: Store prepared vials in refrigerator at -80℃.

However, for the Chinese population, the bottlenecks are as follows:

1. 注意这里列表的写法 页面字会变深 突出内容 ol是编数字的 nts
2. calcium-supplementing pills daste
3. The body's absorptioes as calcium intake increases
4. Vegetables conta列表的写法 页面字会变深 突出内容 ol是编数字的 ul是没有标数字的列表

However, the currently popular calcium supplement method on the market is either to adjust diet or to consume a lot of calcium supplementing pills. For the elderly, they suffer from poor memory or neglect the importance of calcium pills, often forgetting to consume the supplement; for children, they often refuse to take calcium pills or to maintain some diet high in calcium, thus requiring the "liquid calcium" method to ensure sufficient calcium intake. Ho可以 继续大段内容

章节内容的分段就用p开p关

章节内容的分段就用p开p关

章节内容的分段就用p开p关 EXAM 外部链接 href尽量使用https

• 注意这里列表的写法 页面字会变深 突出内容 ul是没有标数字的列表 nts
• calcium-supple menting pills don’t haat taste
• Vegetables conta列表的写法 页面字会变深 突出内容 ol是编数字的 ul是没有标数字的列表

章节内容的分段就用p开p关

引入文字 ion.

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3. The expression level of short peptides has a threshold, preventing any excessive calcium absorption that might lead to hypercalcemia 注意句子超长后的回折
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Header 1 这是igem hq的表格的写法	Header 2
Content A 1	Content B 1
Content A 2	Content B 2 这是igem hq的表格的写法。表格都需要说明，之前之后都会有p来文字

Name	Item Name	Item Price
Alvin	Eclair	$0.87
Alan	Jellybean	$3.76
Jonathan	Lollipop	$7.00

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