Coldplayer (Talk | contribs) |
Coldplayer (Talk | contribs) |
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332089">BBa_K3332089</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332089">BBa_K3332089</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
− | <td>pLtetO-1 promoter_RBS1-LacI-ssrAtag(mf-lon)-B0015-Ptrc-2 derivative | + | <td>pLtetO-1 promoter_RBS1-LacI-ssrAtag(mf-lon)-B0015-Ptrc-2 derivative promoter-B0034-ECFP-B0015</td> |
<td>Ruijie Mo</td> | <td>Ruijie Mo</td> | ||
<td>2511</td> | <td>2511</td> | ||
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332099">BBa_K3332099</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332099">BBa_K3332099</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
− | <td>J23100-B0034-phnE<sup>1</sup>E<sup>2</sup>-J23100-B0034-phnO-RNAi sequence of phnF-RNAi | + | <td>J23100-B0034-phnE<sup>1</sup>E<sup>2</sup>-J23100-B0034-phnO-RNAi sequence of phnF-RNAi sequence of phnJ-J23100-B0034-phnJ</td> |
<td>Shiyi Zhai</td> | <td>Shiyi Zhai</td> | ||
<td>4674</td> | <td>4674</td> |
Revision as of 02:38, 24 October 2020
This year, among the 41 parts designed, ten are basic parts and the other are composite ones. All of the parts are designed by ourselves, and most of them have been tested to insure their reliability. You can click here to learn more about our project to see their specific function, or click on the part numbers when browse website ( parts.igem.org ).
Among the ten basic parts designed this year by ourselves, BBa_K2922040 is our favorite one, which comes from human genomes. The gene enables engineering bacteria to form a vesicle which will have great applications in the enrichment of substrate (such as metal ions) through extracellular membrane and the enhancement of cell stress resistance. Besides, many other basic parts have also been designed to construct our project.
Favorite | Name | Type | Description | Designer | Length |
---|---|---|---|---|---|
R | BBa_K3332011 | Coding | grhpr-his-tag | Xiaoyu Wang | 1005 |
BBa_K3332000 | Coding | inap-his-tag | Shuling Xiao | 1167 | |
BBa_K3332001 | Coding | his-tag-inap | Shuling Xiao | 1167 | |
BBa_K3332002 | Coding | inpnc-inap | Shuling Xiao | 2128 | |
BBa_K3332003 | Coding | lpp-ompA-inap | Shuling Xiao | 1614 | |
BBa_K3332004 | Coding | inap-aida | Shuling Xiao | 2631 | |
BBa_K3332005 | Coding | inap-brkA | Shuling Xiao | 2547 | |
BBa_K3332006 | Coding | gox-his-tag | Xiaoyu Wang | 951 | |
BBa_K3332007 | Coding | inpnc-gox | Xiaoyu Wang | 1908 | |
BBa_K3332008 | Coding | lpp-ompA-gox | Xiaoyu Wang | 1398 | |
BBa_K3332009 | Coding | gox-aida | Xiaoyu Wang | 2415 | |
BBa_K3332010 | Coding | gox-brkA | Xiaoyu Wang | 2331 | |
BBa_K3332012 | Coding | inpnc-grhpr | Shi Zhang | 1962 | |
BBa_K3332013 | Coding | lpp-ompA-grhpr | Shi Zhang | 1452 | |
BBa_K3332014 | Coding | grhpr-aida | Shi Zhang | 2469 | |
BBa_K3332015 | Coding | grhpr-brkA | Shi Zhang | 2385 | |
BBa_K3332016 | Coding | inpnc-egfp | Shi Zhang | 1695 | |
BBa_K3332017 | Coding | lpp-ompA-egfp | Shi Zhang | 1185 | |
BBa_K3332018 | Coding | egfp-aida | Yixian Zheng | 2199 | |
BBa_K3332019 | Coding | egfp-brkA | Yixian Zheng | 2252 | |
BBa_K3332020 | Coding | phnE1 | Yixian Zheng | 963 | |
BBa_K3332021 | Coding | phnE2 | Yixian Zheng | 1518 | |
BBa_K3332022 | Coding | phnC | Yixian Zheng | 840 | |
BBa_K3332023 | Coding | phnD | Yixian Zheng | 906 | |
BBa_K3332024 | Coding | phnJ | Zhichun Yang | 846 | |
BBa_K3332025 | Coding | phnJ_mut21 | Zhichun Yang | 846 | |
BBa_K3332026 | Coding | phnJ_mut40&16 | Zhichun Yang | 846 | |
BBa_K3332027 | Coding | phnJ_mut45 | Zhichun Yang | 846 | |
BBa_K3332028 | Coding | phnO-his-tag | Jisheng Xie | 483 | |
BBa_K3332029 | DNA | ompA 5'UTR-phnF 0.97-hfp binding sequence | Zhichun Yang | 223 | |
BBa_K3332030 | DNA | ompA 5'UTR-phnF 0.85-hfp binding sequence | Ruomeng Bi | 223 | |
BBa_K3332031 | DNA | ompA 5'UTR-phnF 0.75-hfp binding sequence | Ruomeng Bi | 223 | |
BBa_K3332032 | DNA | ompA 5'UTR-phnJ 0.94-hfp binding sequence | Ruomeng Bi | 223 | |
BBa_K3332033 | DNA | ompA 5'UTR-phnJ 0.69-hfp binding sequence | Ruomeng Bi | 223 | |
BBa_K3332034 | DNA | pLtetO-1 promoter | Ruomeng Bi | 142 | |
BBa_K3332035 | Coding | E0020-ssrAtag(mf-lon) | Ruomeng Bi | 819 | |
BBa_K3332036 | RBS | RBS1 | Jinzhu Mao | 9 | |
BBa_K3332037 | Coding | LacI-ssrAtag(mf-lon) | Jinzhu Mao | 1185 | |
BBa_K3332038 | DNA | Ptrc-2 promoter | Jinzhu Mao | 91 | |
BBa_K3332039 | Coding | tetR | Jinzhu Mao | 624 | |
BBa_K3332040 | DNA | Ptrc-2 derivative promoter | Ruomeng Bi | 130 | |
BBa_K3332042 | DNA | Formaldehyde_derivative-1 promoter | Jisheng Xie | 581 | |
BBa_K3332043 | DNA | Formaldehyde_derivative-2 promoter | Zinuo Huang | 645 | |
BBa_K3332044 | DNA | Formaldehyde_derivative-3 promoter | Xingyu Chen | 631 |
A series of composite parts have been designed this year, and most of them are useful for our project. And our favorite composite part is BBa_K2922009 , which contains the sequence of the protein Kil. Kil protein, which accumulates in the periplasmic space of the bacteria, can increase the periplasmic space and improved the membrane permeability of bacteria. Therefore, the protein inside the bacteria can secrete out of bacteria with the help from Kil protein. The use of proper intensity of the promoter can not only achieve the purpose of secretion but also prevent side effects of cell lysis and death. After a series of experiments, J23109 is determined as the most proper promoter.
Favorite | Name | Type | Description | Designer | Length |
---|---|---|---|---|---|
R | BBa_K3332060 | Composite | J23100-B0034-grhpr-brkA | Xiaoyu Wang | 2583 |
BBa_K3332045 | Composite | J23100-B0034-inap-his-tag-B0015 | Shengyang Zhang | 1365 | |
BBa_K3332046 | Composite | J23100-B0034-his-tag-inap-B0015 | Shengyang Zhang | 1365 | |
BBa_K3332047 | Composite | J23100-B0034-inpnc-inap | Shengyang Zhang | 2328 | |
BBa_K3332048 | Composite | J23100-B0034-lpp-ompA-inap | Shengyang Zhang | 1812 | |
BBa_K3332049 | Composite | J23100-B0034-inap-aida-B0015 | Shengyang Zhang | 2829 | |
BBa_K3332050 | Composite | J23100-B0034-inap-brkA | Shengyang Zhang | 2745 | |
BBa_K3332051 | Composite | J23100-B0034-gox-his-tag-B0015 | Yangqi Deng | 1149 | |
BBa_K3332052 | Composite | J23100-B0034-inpnc-gox | Yangqi Deng | 2106 | |
BBa_K3332053 | Composite | J23100-B0034-lpp-ompA-gox | Yangqi Deng | 1596 | |
BBa_K3332054 | Composite | J23100-B0034-gox-aida-B0015 | Yangqi Deng | 2613 | |
BBa_K3332055 | Composite | J23100-B0034-gox-brkA | Yangqi Deng | 2529 | |
BBa_K3332056 | Composite | J23100-B0034-grhpr-his-tag-B0015 | Yangqi Deng | 1203 | |
BBa_K3332057 | Composite | J23100-B0034-inpnc-grhpr-B0015 | Qingliu Wang | 2160 | |
BBa_K3332058 | Composite | J23100-B0034-lpp-ompA-grhpr | Qingliu Wang | 1650 | |
BBa_K3332059 | Composite | J23100-B0034-grhpr-aida | Qingliu Wang | 2667 | |
BBa_K3332061 | Composite | J23100-B0034-inpnc-egfp-B0015 | Qingliu Wang | 1893 | |
BBa_K3332062 | Composite | J23100-B0034-lpp-ompA-egfp | Qingliu Wang | 1383 | |
BBa_K3332063 | Composite | J23100-B0034-egfp-aida-B0015 | Fude Chen | 2397 | |
BBa_K3332064 | Composite | J23100-B0034-egfp-brkA-B0015 | Yuan Li | 2450 | |
BBa_K3332065 | Composite | J23100-B0034-phnE1 | Gezhi Xiao | 1024 | |
BBa_K3332066 | Composite | J23100-B0034-phnE2 | Meihao Ou | 1579 | |
BBa_K3332067 | Composite | J23100-B0034-phnE1-B0034-phnE2 | Yuan Li | 2568 | |
BBa_K3332068 | Composite | J23100-B0034-phnJ | Tianyu Feng | 907 | |
BBa_K3332069 | Composite | J23100-B0034-phnJ_mut21 | Siru Zhou | 907 | |
BBa_K3332070 | Composite | J23100-B0034-phnJ_mut40&16 | Siru Zhou | 907 | |
BBa_K3332071 | Composite | J23100-B0034-phnJ_mut45 | Siru Zhou | 907 | |
BBa_K3332072 | Composite | J23100-B0034-phnO-his-tag | Siru Zhou | 681 | |
BBa_K3332073 | Composite | J23101-ompA 5'UTR-phnF 0.97-hfp binding sequence | Siru Zhou | 286 | |
BBa_K3332074 | Composite | J23101-ompA 5'UTR-phnF 0.85-hfp binding sequence | Siru Zhou | 286 | |
BBa_K3332075 | Composite | J23101-ompA 5'UTR-phnF 0.75-hfp binding sequence | Meihao Ou | 286 | |
BBa_K3332076 | Composite | J23101-ompA 5'UTR-phnJ 0.94-hfp binding sequence | Meihao Ou | 286 | |
BBa_K3332077 | Composite | J23101-ompA 5'UTR-phnJ 0.69-hfp binding sequence | Meihao Ou | 286 | |
BBa_K3332078 | Composite | Formaldehyde promoter-Inverter-MazF | Meihao Ou | 2072 | |
BBa_K3332079 | Composite | pBAD/araC promoter-Inverter-EYFP | Meihao Ou | 2476 | |
BBa_K3332080 | Composite | pBAD/araC promoter-cI repressor | Meihao Ou | 2148 | |
BBa_K3332081 | Composite | pBAD/araC promoter-Inverter-MazF | Shichen Geng | 2687 | |
BBa_K3332082 | Composite | pBAD/araC promoter-B0034-EYFP | Shichen Geng | 2096 | |
BBa_K3332083 | Composite | pBAD/araC promoter-B0034-MazF | Shichen Geng | 1712 | |
BBa_K3332084 | Composite | pLtetO-1-B0034-ECFP-B0015 | Shichen Geng | 1028 | |
BBa_K3332085 | Composite | pLtetO-1-ECFP-B0015-J23106-B0034-tetR-B0015 | Shichen Geng | 1921 | |
BBa_K3332086 | Composite | Ptrc-2 promoter-B0034-ECFP-B0015 | Shichen Geng | 977 | |
BBa_K3332087 | Composite | Ptrc-2 derivative promoter-B0034-ECFP-B0015 | Ruijie Mo | 1016 | |
BBa_K3332088 | Composite | pLtetO-1 promoter-RBS1-LacI-ssrAtag(mf-lon)-B0015-Ptrc-2 promoter-B0034-ECFP-B0015 |
Ruijie Mo | 2472 | |
BBa_K3332089 | Composite | pLtetO-1 promoter_RBS1-LacI-ssrAtag(mf-lon)-B0015-Ptrc-2 derivative promoter-B0034-ECFP-B0015 | Ruijie Mo | 2511 | |
BBa_K3332090 | Composite | Formaldehyde promoter-B0034-ECFP | Ruijie Mo | 1481 | |
BBa_K3332091 | Composite | Formaldehyde_derivative-1 promoter-ECFP | Ruijie Mo | 1467 | |
BBa_K3332092 | Composite | Formaldehyde_derivative-3 promoter-ECFP | Shiyi Zhai | 1517 | |
BBa_K3332093 | Composite | Formaldehyde_derivative-2 promoter-ECFP | Ruijie Mo | 1531 | |
BBa_K3332097 | Composite | J23100-B0034-phnE1E2-J23100-B0034-phnJ | Shiyi Zhai | 3463 | |
BBa_K3332098 | Composite | RNAi sequence of phnF-RNAi sequence of phnJ | Shiyi Zhai | 494 | |
BBa_K3332099 | Composite | J23100-B0034-phnE1E2-J23100-B0034-phnO-RNAi sequence of phnF-RNAi sequence of phnJ-J23100-B0034-phnJ | Shiyi Zhai | 4674 | |
BBa_K33320100 | Composite | J23100-B0034-phnO-J23100-B0034-phnE1E2-RNAi sequence of phnF-RNAi sequence of phnJ-J23100-B0034-phnJ | Shiyi Zhai | 4674 |
This year, we have formulated a Part Collection related to secretion of intracellular proteins, which includes two major parts: one is secretion achieved with the help of YebF in the form of fusion proteins, the other is the expression of Kil protein by promoters of different intensities, thus changing cell permeability to different degrees and finally realizing secretion. For our Part Collection, the efficiency of three different intracellular proteins we utilized were well tested, recorded in detail and completely uploaded to the registry, which would be instructive in the field of design and characterization for many teams.
Register Name | Code used in Lab | Description | Types | Lenth |
---|---|---|---|---|
BBa_K2922000 | y-bgl1A | The protein beta-glucosidase with protein YebF fused to its N-terminus by GS Linker. |
Coding | 1764 |
BBa_K2922001 | y-cenA | The protein endoglucanase A with protein YebF fused to its N-terminus by GS Linker. |
Coding | 1731 |
BBa_K2922002 | y-cex | The protein exoglucanase with protein YebF fused to its N-terminus by GS Linker. |
Coding | 1839 |
BBa_K2922009 | 109-kil | The expression of Kil protein by J23109 promoter |
Composite | 248 |
BBa_K2922010 | 112-kil | The expression of Kil protein by J23112 promoter |
Composite | 248 |
BBa_K2922011 | 114-kil | The expression of Kil protein by J23114 promoter |
Composite | 248 |
BBa_K2922012 | 109-kil-T7-bgl1A | The secretion of beta-glucosidase by BBa_K2922009. |
Composite | 1760 |
BBa_K2922013 | 112-kil-T7-bgl1A | The secretion of beta-glucosidase by BBa_K2922010. |
Composite | 1760 |
BBa_K2922014 | 114-kil-T7-bgl1A | The secretion of beta-glucosidase by BBa_K2922011. |
Composite | 1760 |
BBa_K2922015 | 109-kil-T7-cenA | The secretion of endoglucanase by BBa_K2922009. |
Composite | 1727 |
BBa_K2922016 | 112-kil-T7-cenA | The secretion of endoglucanase by BBa_K2922010. |
Composite | 1727 |
BBa_K2922017 | 114-kil-T7-cenA | The secretion of endoglucanase by BBa_K2922011. |
Composite | 1727 |
BBa_K2922018 | 109-kil-T7-cex | The secretion of exoglucanase by BBa_K2922009. |
Composite | 1835 |
BBa_K2922019 | 112-kil-T7-cex | The secretion of exoglucanase by BBa_K2922010. |
Composite | 1835 |
BBa_K2922020 | 114-kil-T7-cex | The secretion of exoglucanase by BBa_K2922011. |
Composite | 1835 |