Difference between revisions of "Team:TU Darmstadt/Project/Backbones"

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                 <a href="#Chapter 1">Backbones Table</a>
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                 <a href="#Backbones">Backbones Table</a>
 
                
 
                
 
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For the <i>in silico</i> design of our experiments for the wetlab we used several <b>different plasmid backbones</b>. Due to COVID-19 we were <b>not able to test any of our constructs in the lab</b>. Therefore, we only planned the shown backbones theoretically. The plasmids would be used for <b>cloning, expression, and genomic integration</b> of our constructs. You can find a list of all plasmids we used for our design and the corresponding parts below.
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For the <i>in silico</i> design of our experiments for the wetlab we used several <b>different plasmid backbones</b>. Due to the COVID-19 pandemic we were <b>not able to test any of our constructs in the lab</b>. Therefore, we only planned the shown backbones theoretically. The plasmids would be used for <b>cloning, expression, and genomic integration</b> of our constructs.
 
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<a  class="anchor" id="Backbones"></a>
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            <h2>
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                Backbone Table
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     <tr>
 
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       <th scope="col">Name</th>
 
       <th scope="col">Name</th>
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       <th scope="col">Size</th>
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       <th scope="col">Size [bp]</th>
 
       <th scope="col">Resistance</th>
 
       <th scope="col">Resistance</th>
 
       <th scope="col">Features</th>
 
       <th scope="col">Features</th>
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       <td scope="row">pET24(+)</th>
 
       <td scope="row">pET24(+)</th>
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       <td>5236 bp</td>
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       <td>5236</td>
 
       <td>Kanamycin</td>
 
       <td>Kanamycin</td>
 
       <td>KanR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, f1 ori, lacI repressor + lacI promoter, T7 promoter + lac operator, 6xHis, T7 terminator</td>
 
       <td>KanR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, f1 ori, lacI repressor + lacI promoter, T7 promoter + lac operator, 6xHis, T7 terminator</td>
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<td><i>E. coli</i></td>
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<td><i>E.&nbsp;coli</i></td>
 
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       <td scope="row">pSEVA3b67Rb</th>
 
       <td scope="row">pSEVA3b67Rb</th>
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       <td>4617 bp</td>
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       <td>4617</td>
 
       <td>Chloramphenicol</td>
 
       <td>Chloramphenicol</td>
 
       <td>high-copy-number pUC ori, pTHT15 ori, repB, mRFP1, rrnB T1 terminator, oriT, lambda t0 terminator </td>
 
       <td>high-copy-number pUC ori, pTHT15 ori, repB, mRFP1, rrnB T1 terminator, oriT, lambda t0 terminator </td>
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       <td><i>E. coli</i>, <i>B. subtilis</i></td>
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       <td><i>E.&nbsp;coli</i>, <i>B.&nbsp;subtilis</i></td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
 
       <td scope="row">pJET1.2</th>
 
       <td scope="row">pJET1.2</th>
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       <td> 2974 bp </td>
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       <td> 2974</td>
 
       <td>Ampicillin</td>
 
       <td>Ampicillin</td>
 
   <td>AmpR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori</td>
 
   <td>AmpR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori</td>
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   <td><i>E. coli</i></td>
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   <td><i>E.&nbsp;coli</i></td>
 
     </tr>
 
     </tr>
 
   </tbody>
 
   </tbody>

Revision as of 12:01, 24 October 2020

image/svg+xml - O O

Backbones

Backbones Table



Backbones Table

For the in silico design of our experiments for the wetlab we used several different plasmid backbones. Due to the COVID-19 pandemic we were not able to test any of our constructs in the lab. Therefore, we only planned the shown backbones theoretically. The plasmids would be used for cloning, expression, and genomic integration of our constructs.

Backbone Table

Name Size [bp] Resistance Features Organism
pET24(+) 5236 Kanamycin KanR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, f1 ori, lacI repressor + lacI promoter, T7 promoter + lac operator, 6xHis, T7 terminator E. coli
pSEVA3b67Rb 4617 Chloramphenicol high-copy-number pUC ori, pTHT15 ori, repB, mRFP1, rrnB T1 terminator, oriT, lambda t0 terminator E. coli, B. subtilis
pJET1.2 2974 Ampicillin AmpR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori E. coli