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− | {{TU_Darmstadt}}{{TU_Darmstadt/Bootstrap}}{{TU_Darmstadt/CSS}}{{TU_Darmstadt/Homepage/SVG_HEADER}}{{TU_Darmstadt/NavBar}}{{TU_Darmstadt/Project/Banner/Backbones}} | + | {{TU_Darmstadt}}{{TU_Darmstadt/Bootstrap}}{{TU_Darmstadt/CSS}}{{TU_Darmstadt/Homepage/SVG_HEADER}}{{TU_Darmstadt/NavBar}}{{TU_Darmstadt/Project/Banner/Backbones}}{{TU_Darmstadt/Homeboy}} |
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<html> | <html> | ||
<!----------SideBar Ignore me ---------> | <!----------SideBar Ignore me ---------> | ||
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<div class="sidenav"> | <div class="sidenav"> | ||
− | <a href="# | + | <a href="#Backbones">Backbones Table</a> |
</div> | </div> | ||
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<div class="col-lg-12 col-xl-8"> | <div class="col-lg-12 col-xl-8"> | ||
<br> | <br> | ||
+ | <a class="anchor" id="scroll"></a> | ||
<div class="TFcontainer"> | <div class="TFcontainer"> | ||
<div class="containertext"> | <div class="containertext"> | ||
− | For the <i>in silico</i> design of our experiments for the wetlab we used several <b>different plasmid backbones</b>. Due to | + | For the <i>in silico</i> design of our experiments for the wetlab we used several <b>different plasmid backbones</b>. Due to the COVID-19 pandemic we were <b>not able to test any of our constructs in the lab</b>. Therefore, we only planned the shown backbones theoretically. The plasmids would be used for <b>cloning, expression, and genomic integration</b> of our constructs. |
</div> | </div> | ||
</div> | </div> | ||
− | <br> | + | <br> |
+ | <a class="anchor" id="Backbones"></a> | ||
+ | <h2> | ||
+ | Backbone Table | ||
+ | </h2> | ||
+ | <div class="headlinebar"> | ||
+ | </div> | ||
<table class="table table-bordered"> | <table class="table table-bordered"> | ||
<thead> | <thead> | ||
<tr> | <tr> | ||
<th scope="col">Name</th> | <th scope="col">Name</th> | ||
− | <th scope="col">Size</th> | + | <th scope="col">Size [bp]</th> |
<th scope="col">Resistance</th> | <th scope="col">Resistance</th> | ||
<th scope="col">Features</th> | <th scope="col">Features</th> | ||
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<tr> | <tr> | ||
<td scope="row">pET24(+)</th> | <td scope="row">pET24(+)</th> | ||
− | <td> | + | <td>5236</td> |
− | <td> | + | <td>Kanamycin</td> |
<td>KanR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, f1 ori, lacI repressor + lacI promoter, T7 promoter + lac operator, 6xHis, T7 terminator</td> | <td>KanR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, f1 ori, lacI repressor + lacI promoter, T7 promoter + lac operator, 6xHis, T7 terminator</td> | ||
− | <td><i>E. coli</i></td> | + | <td><i>E. coli</i></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td scope="row">pSEVA3b67Rb</th> | <td scope="row">pSEVA3b67Rb</th> | ||
− | <td> | + | <td>4617</td> |
− | <td> | + | <td>Chloramphenicol</td> |
− | <td> | + | <td>high-copy-number pUC ori, pTHT15 ori, repB, mRFP1, rrnB T1 terminator, oriT, lambda t0 terminator </td> |
− | <td><i>E. coli</i>, <i>B. subtilis</i></td> | + | <td><i>E. coli</i>,<br> <i>B. subtilis</i></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td scope="row">pJET1.2</th> | <td scope="row">pJET1.2</th> | ||
− | <td> 2974 | + | <td> 2974</td> |
<td>Ampicillin</td> | <td>Ampicillin</td> | ||
<td>AmpR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori</td> | <td>AmpR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori</td> | ||
− | <td><i>E. coli | + | <td><i>E. coli</i></td> |
</tr> | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
+ | <br> | ||
</div> | </div> |
Latest revision as of 13:17, 24 October 2020
For the in silico design of our experiments for the wetlab we used several different plasmid backbones. Due to the COVID-19 pandemic we were not able to test any of our constructs in the lab. Therefore, we only planned the shown backbones theoretically. The plasmids would be used for cloning, expression, and genomic integration of our constructs.
Backbone Table
Name | Size [bp] | Resistance | Features | Organism |
---|---|---|---|---|
pET24(+) | 5236 | Kanamycin | KanR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, f1 ori, lacI repressor + lacI promoter, T7 promoter + lac operator, 6xHis, T7 terminator | E. coli |
pSEVA3b67Rb | 4617 | Chloramphenicol | high-copy-number pUC ori, pTHT15 ori, repB, mRFP1, rrnB T1 terminator, oriT, lambda t0 terminator | E. coli, B. subtilis |
pJET1.2 | 2974 | Ampicillin | AmpR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori | E. coli |