Difference between revisions of "Team:TU Darmstadt/Project/Backbones"

 
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                 <a href="#Chapter 1">Chapter 1</a>
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                 <a href="#Backbones">Backbones Table</a>
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                <a href="#Chapter 3">Chapter 3</a>
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For the <i>in silico</i> design of our experiments for the wetlab we used several <b>different plasmid backbones</b>. Due to the COVID-19 pandemic we were <b>not able to test any of our constructs in the lab</b>. Therefore, we only planned the shown backbones theoretically. The plasmids would be used for <b>cloning, expression, and genomic integration</b> of our constructs.
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            <h2>
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                Backbone Table
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      <th scope="col">Name</th>
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      <th scope="col">Size [bp]</th>
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      <th scope="col">Resistance</th>
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      <th scope="col">Features</th>
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      <th scope="col">Organism</th>
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      <td scope="row">pET24(+)</th>
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      <td>5236</td>
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      <td>Kanamycin</td>
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      <td>KanR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, f1 ori, lacI repressor + lacI promoter, T7 promoter + lac operator, 6xHis, T7 terminator</td>
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<td><i>E.&nbsp;coli</i></td>
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      <td scope="row">pSEVA3b67Rb</th>
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      <td>4617</td>
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      <td>Chloramphenicol</td>
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      <td>high-copy-number pUC ori, pTHT15 ori, repB, mRFP1, rrnB T1 terminator, oriT, lambda t0 terminator </td>
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      <td><i>E.&nbsp;coli</i>,<br> <i>B.&nbsp;subtilis</i></td>
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      <td scope="row">pJET1.2</th>
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      <td> 2974</td>
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      <td>Ampicillin</td>
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  <td>AmpR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori</td>
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  <td><i>E.&nbsp;coli</i></td>
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Latest revision as of 13:17, 24 October 2020

image/svg+xml - O O

Backbones

Backbones Table



Backbones Table

For the in silico design of our experiments for the wetlab we used several different plasmid backbones. Due to the COVID-19 pandemic we were not able to test any of our constructs in the lab. Therefore, we only planned the shown backbones theoretically. The plasmids would be used for cloning, expression, and genomic integration of our constructs.

Backbone Table

Name Size [bp] Resistance Features Organism
pET24(+) 5236 Kanamycin KanR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, f1 ori, lacI repressor + lacI promoter, T7 promoter + lac operator, 6xHis, T7 terminator E. coli
pSEVA3b67Rb 4617 Chloramphenicol high-copy-number pUC ori, pTHT15 ori, repB, mRFP1, rrnB T1 terminator, oriT, lambda t0 terminator E. coli,
B. subtilis
pJET1.2 2974 Ampicillin AmpR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori E. coli