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| − | {{TU_Darmstadt}}{{TU_Darmstadt/Bootstrap}}{{TU_Darmstadt/CSS}} | + | {{TU_Darmstadt}}{{TU_Darmstadt/Bootstrap}}{{TU_Darmstadt/CSS}}{{TU_Darmstadt/Homepage/SVG_HEADER}}{{TU_Darmstadt/NavBar}}{{TU_Darmstadt/Project/Banner/Backbones}}{{TU_Darmstadt/Homeboy}} |
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| − | {{TU_Darmstadt/NavBar}}
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| − | {{TU_Darmstadt/Project/Banner}}
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| | <html> | | <html> |
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| | <!----------SideBar Ignore me ---------> | | <!----------SideBar Ignore me ---------> |
| | <div class="container-fluid"> | | <div class="container-fluid"> |
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| | <div class="sidenav"> | | <div class="sidenav"> |
| − | <a href="#Chapter 1">University</a> | + | <a href="#Backbones">Backbones Table</a> |
| − | <a href="#Chapter 2">Experts</a>
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| − | <a href="#Chapter 3">Former iGEMers</a>
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| − | <a href="#Chapter 4">Others</a>
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| | </div> | | </div> |
| | </div> | | </div> |
| | <!----------SideBar Ignore me ---------> | | <!----------SideBar Ignore me ---------> |
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| − | <div class="col-10 col-sm-12 col-xl-10"> | + | <div class="col-lg-12 col-xl-8"> |
| − | | + | <br> |
| | + | <a class="anchor" id="scroll"></a> |
| | <div class="TFcontainer"> | | <div class="TFcontainer"> |
| | <div class="containertext"> | | <div class="containertext"> |
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| + | For the <i>in silico</i> design of our experiments for the wetlab we used several <b>different plasmid backbones</b>. Due to the COVID-19 pandemic we were <b>not able to test any of our constructs in the lab</b>. Therefore, we only planned the shown backbones theoretically. The plasmids would be used for <b>cloning, expression, and genomic integration</b> of our constructs. |
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| | </div> | | </div> |
| − | <div class=" containerimg"> | + | </div> |
| − | <figure> | + | <br> |
| − | <img src="https://static.igem.org/mediawiki/2020/d/d5/T--TU_Darmstadt--CD-ROM.jpg" alt="figure"> | + | <a class="anchor" id="Backbones"></a> |
| − | <figcaption id="Figure#">Figure 1: This could be a collabo picture </figcaption>
| + | <h2> |
| − | </figure>
| + | Backbone Table |
| − | </div>
| + | </h2> |
| − | </div> | + | <div class="headlinebar"> |
| − | | + | </div> |
| − | | + | <table class="table table-bordered"> |
| − | | + | <thead> |
| − | | + | <tr> |
| − | | + | <th scope="col">Name</th> |
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| + | <th scope="col">Size [bp]</th> |
| − | | + | <th scope="col">Resistance</th> |
| − | <div class="headline">
| + | <th scope="col">Features</th> |
| − | <h1>
| + | <th scope="col">Organism</th> |
| − | University
| + | </tr> |
| − | </h1>
| + | </thead> |
| − | | + | <tbody> |
| − | </div>
| + | <tr> |
| − | | + | <td scope="row">pET24(+)</th> |
| − | <!----------Insert TEXT Here ---------> | + | <td>5236</td> |
| − | | + | <td>Kanamycin</td> |
| − | <div class="TFcontainer"> | + | <td>KanR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, f1 ori, lacI repressor + lacI promoter, T7 promoter + lac operator, 6xHis, T7 terminator</td> |
| − | <div class="containertext">
| + | <td><i>E. coli</i></td> |
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| + | </tr> |
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| + | <tr> |
| | + | <td scope="row">pSEVA3b67Rb</th> |
| | + | <td>4617</td> |
| | + | <td>Chloramphenicol</td> |
| | + | <td>high-copy-number pUC ori, pTHT15 ori, repB, mRFP1, rrnB T1 terminator, oriT, lambda t0 terminator </td> |
| | + | <td><i>E. coli</i>,<br> <i>B. subtilis</i></td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td scope="row">pJET1.2</th> |
| | + | <td> 2974</td> |
| | + | <td>Ampicillin</td> |
| | + | <td>AmpR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori</td> |
| | + | <td><i>E. coli</i></td> |
| | + | </tr> |
| | + | </tbody> |
| | + | </table> |
| | <br> | | <br> |
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