Difference between revisions of "Team:TU Darmstadt/Project/Backbones"

 
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                 <a href="#Chapter 1">University</a>
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                 <a href="#Backbones">Backbones Table</a>
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                <a href="#Chapter 2">Experts</a>
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                <a href="#Chapter 3">Former iGEMers</a>
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                <a href="#Chapter 4">Others</a>
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                Backbones
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For the <i>in silico</i> design of our experiments for the wetlab we used several different plasmid backbones. Due to Covid-19 we were not able to test any of our constructs in the lab. Therefore, we only planned the shown backbones theoretically. The plasmids would be used for cloning, expression, and genomic integration of our constructs. You can find a list of all plasmids we used for our design and the corresponding parts below.
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For the <i>in silico</i> design of our experiments for the wetlab we used several <b>different plasmid backbones</b>. Due to the COVID-19 pandemic we were <b>not able to test any of our constructs in the lab</b>. Therefore, we only planned the shown backbones theoretically. The plasmids would be used for <b>cloning, expression, and genomic integration</b> of our constructs.
 
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    <br>
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<div class="container-noborders">
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<a  class="anchor" id="Backbones"></a>
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         <div class="table-responsive-sm">
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            <h2>
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          <table class="table table-light">
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                Backbone Table
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            <tr>
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            </h2>
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              <th scope="col">Name</th>
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         <div class="headlinebar">
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              <th scope="col">Resistance</th>
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                </div> 
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              <th scope="col">Size</th>
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<table class="table table-bordered">
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              <th scope="col">Features <b>This is a placeholder</b></th>
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  <thead>
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              <th scope="col">Used for the following parts</th>
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    <tr>
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            </tr>
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      <th scope="col">Name</th>
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            <tr>
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      <th scope="col">Size [bp]</th>
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              <td>pACYCT2</td>
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      <th scope="col">Resistance</th>
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              <td>Cmp</td>
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      <th scope="col">Features</th>
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              <td>3975 bp</td>
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      <th scope="col">Organism</th>
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              <td>
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    </tr>
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                cat promoter, CmR, p15A ori, lacI repressor + lacI promoter, T7
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  </thead>
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                promoter, lac operator, T7 terminator
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  <tbody>
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              </td>
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    <tr>
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              <td><a href=" http://parts.igem.org/Part:BBa_K3187000 "
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      <td scope="row">pET24(+)</th>
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                    target="_blank"> BBa_K3187000</a>, <a href=" http://parts.igem.org/Part:BBa_K3187001 "
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      <td>5236</td>
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                    target="_blank"> BBa_K3187001</a>, <a href=" http://parts.igem.org/Part:BBa_K3187003"
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      <td>Kanamycin</td>
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                    target="_blank"> BBa_K3187003</a>, <a href=" http://parts.igem.org/Part:BBa_K3187004" target="_blank"> BBa_K3187004</a>
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      <td>KanR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, f1 ori, lacI repressor + lacI promoter, T7 promoter + lac operator, 6xHis, T7 terminator</td>
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</td>
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<td><i>E.&nbsp;coli</i></td>
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            </tr>
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    </tr>
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            <tr></tr>
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    <tr>
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            <tr>
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      <td scope="row">pSEVA3b67Rb</th>
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              <td>pDESTara2</td>
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      <td>4617</td>
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              <td>Amp</td>
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      <td>Chloramphenicol</td>
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              <td>4556 bp</td>
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      <td>high-copy-number pUC ori, pTHT15 ori, repB, mRFP1, rrnB T1 terminator, oriT, lambda t0 terminator </td>
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              <td>AmpR, araBAD promoter, araC, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, rrnB T1 and T7Te terminator
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      <td><i>E.&nbsp;coli</i>,<br> <i>B.&nbsp;subtilis</i></td>
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                promoter, lac operator, T7 terminator</td>
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    </tr>
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              <td><a href=" http://parts.igem.org/Part:BBa_K3187010 "
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    <tr>
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                    target="_blank"> BBa_K3187010</a>
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      <td scope="row">pJET1.2</th>
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</td>
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      <td> 2974</td>
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            </tr>
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      <td>Ampicillin</td>
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            <tr>
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  <td>AmpR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori</td>
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              <td>pET24</td>
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  <td><i>E.&nbsp;coli</i></td>
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              <td>Kan</td>
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    </tr>
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              <td>5236 bp</td>
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  </tbody>
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              <td>
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</table>
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                KanR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, f1 ori, lacI
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<br>
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                repressor + lacI promoter, T7 promoter + lac operator, 6xHis, T7
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                terminator
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              </td>
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              <td><a href=" http://parts.igem.org/Part:BBa_K3187000"
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                    target="_blank"> BBa_K3187000</a>, <a href=" http://parts.igem.org/Part:BBa_K3187002 "
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                    target="_blank"> BBa_K3187002</a>, <a href=" http://parts.igem.org/Part:BBa_K3187005 "
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                    target="_blank"> BBa_K3187005</a>, <a href=" http://parts.igem.org/Part:BBa_K3187006 "
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                    target="_blank"> BBa_K3187006</a>, <a href=" http://parts.igem.org/Part:BBa_K3187008 "
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                    target="_blank"> BBa_K3187008</a>
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</td>
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            </tr>
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            <tr>
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              <td>pSB1C3</td>
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              <td>Cmp</td>
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              <td>2070 bp</td>
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              <td>
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                CmR, lambda t0 terminator, high-copy-number
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                ColE1/ pMB1/ pBR322/ pUC ori, his operton Terminator, BioBrick Pre-
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                and Suffix
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              </td>
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              <td><a href=" http://parts.igem.org/Part:BBa_K3187014 "
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                    target="_blank"> BBa_K3187014</a>, <a href=" http://parts.igem.org/Part:BBa_K3187015 "
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                    target="_blank"> BBa_K3187015</a>
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</td>
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            </tr>
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            <tr>
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              <td>pTeTW3con2</td>
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              <td>Cmp</td>
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              <td>4555 bp</td>
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              <td>
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                cat promoter, CmR, p15A ori, lacI repressor + lacI promoter,
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                tetR/tetA promoter, tetR repressor, tet operator, T7 promoter +
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                lac operator, T7 terminator
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              </td>
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              <td><a href=" http://parts.igem.org/Part:BBa_K3187011"
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                    target="_blank"> BBa_K3187011</a>, <a href=" http://parts.igem.org/Part:BBa_K3187012"
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                    target="_blank"> BBa_K3187012</a>, <a href=" http://parts.igem.org/Part:BBa_K3187013"
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                    target="_blank"> BBa_K3187013</a>
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</td>
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            </tr>
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            <tr>
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            </tr>
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          </table>
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Latest revision as of 13:17, 24 October 2020

image/svg+xml - O O

Backbones

Backbones Table



Backbones Table

For the in silico design of our experiments for the wetlab we used several different plasmid backbones. Due to the COVID-19 pandemic we were not able to test any of our constructs in the lab. Therefore, we only planned the shown backbones theoretically. The plasmids would be used for cloning, expression, and genomic integration of our constructs.

Backbone Table

Name Size [bp] Resistance Features Organism
pET24(+) 5236 Kanamycin KanR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, f1 ori, lacI repressor + lacI promoter, T7 promoter + lac operator, 6xHis, T7 terminator E. coli
pSEVA3b67Rb 4617 Chloramphenicol high-copy-number pUC ori, pTHT15 ori, repB, mRFP1, rrnB T1 terminator, oriT, lambda t0 terminator E. coli,
B. subtilis
pJET1.2 2974 Ampicillin AmpR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori E. coli