一句短语概括整个页面，不要用overview这类无实际意义的词或词组
页面摘要，或者是单项奖核心点 Our project aims at converting engineered Our project aims at converting engineered Our project aims at converting engineered Our project aims at converting engi neered Our project aims at converting engineered Our project aims at converting engineered Our project aims at converting engineered Our project aims at converting engineered
每个章节标题用h2 为了搜索引擎全页面只能一个h1标签
Engineering of Kill Switch
添加一个概述
Brainstorm
To be responsible for the environment, we considered it is necessary to introduce a Kill Switch to deprive of the survivability of engineered E. coli outside human bodies. Kill Switch is a gene device to eliminate bacteria in a short period of time when engineered bacteria escape from the set conditions. In the context of our project, the set condition is human intestine and the condition under which we wanted our bacteria to die is the outside environment. Therefore, we firstly come up with a distinguishing condition in intestine and in nature – glucose concentration. Glucose concentration is higher than the environment and is a very general condition that may enable the Kill Switch apply to many situations.
Tentative design
After searching literatures, we learned several special glucose-sensitive promoters could facilitate different state of expression in the two conditions. We found two glucose-sensitive promoters in the part registry ( rpoH P5 and PT-αCRP ). For example, PT-αCRP can turn on downstream gene precisely and autonomously in response to glucose limitation, and for this reason it is also called glucose-starvation promoter. So, we imagined our Kill Switch to be a glucose-starvation promoter followed by a toxic protein gene.
Consultant
We further researched parameters of the two promoters to forecast the efficiency of the Kill Switch. For one thing, the trigger concentration of them are both 0.01-0.05%, while the glucosuria level of diabetics and pregnant women can be far higher than that [1]. However, our target consumer is the elderly who also often have diabetes, glucose-starvation promoter may not be activated effectively in their excrement. For the other thing, the model of rpoH P5 made by BNU-China demonstrated that glucose concentration in intestine would drop under o.o5% within 2 hours after meals, which impeded persistent calcium supplement function [2]. For more practical information, we consulted the designer of rpoH P5 from Beijing Normal University. He didn’t consider glucose concentration as a specific signal to trigger elimination outside the body and also recommended us try toxin/antitoxin systems to enable more flexible regulation. (Click here to see our Integrated Human Practices page for detailed information.)
Brainstorm
All members of our team have taken online courses in biosafety, such as hazardous chemicals, laboratory waste, special equipment safety, accident handling, and passed the laboratory safety examination required by the Assets and Security Department of our university before we entering the lab.
Brainstorm
All members of our team have taken online courses in biosafety, such as hazardous chemicals, laboratory waste, special equipment safety, accident handling, and passed the laboratory safety examination required by the Assets and Security Department of our university before we entering the lab.
Brainstorm
All members of our team have taken online courses in biosafety, such as hazardous chemicals, laboratory waste, special equipment safety, accident handling, and passed the laboratory safety examination required by the Assets and Security Department of our university before we entering the lab.
每个章节标题用h2 为了搜索引擎全页面只能一个h1标签
在宽屏，这部分内容占页面1/2；窄屏就是全幅。添加了column同时 图1/2 + 字1/2 正好宽屏的一幅。这个代码不在 https://2020.igem.org/Resources/Template_Documentation 但是是支持的 China has an estimated 70 million osteo porosis patien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste oporosis patienChina has an estimated 70 million osteoporosis patien
每个章节标题用h2 为了搜索引擎全页面只能一个h1标签
在宽屏，这部分内容占页面1/3；窄屏就是全幅。添加了column同时 图1/3 + 字2/3 正好宽屏的一幅。 China has an estimated 70 million osteo porosis patien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste oporosis patienChina has an estimated 70 million osteoporosis patien
However, for the Chinese population, the bottlenecks are as follows:
- 注意这里列表的写法 页面字会变深 突出内容 ol是编数字的 nts
- calcium-supplementing pills daste
- The body's absorptioes as calcium intake increases
- Vegetables conta列表的写法 页面字会变深 突出内容 ol是编数字的 ul是没有标数字的列表
However, the currently popular calcium supplement method on the market is either to adjust diet or to consume a lot of calcium supplementing pills. For the elderly, they suffer from poor memory or neglect the importance of calcium pills, often forgetting to consume the supplement; for children, they often refuse to take calcium pills or to maintain some diet high in calcium, thus requiring the "liquid calcium" method to ensure sufficient calcium intake. Ho可以 继续大段内容
章节内容的分段就用p开p关，额外需要关注的可以套一个highlight的框，有多种版本
章节内容的分段就用p开p关
章节内容的分段就用p开p关，额外需要关注的可以套一个highlight的框，细看 https://2020.igem.org/Resources/Template_Documentation#highlight
章节内容的分段就用p开p关
章节内容的分段就用p开p关 EXAM 外部链接 href尽量使用https
- 注意这里列表的写法 页面字会变深 突出内容 ul是没有标数字的列表 nts
- calcium-supple menting pills don’t haat taste
- Vegetables conta列表的写法 页面字会变深 突出内容 ol是编数字的 ul是没有标数字的列表
章节内容的分段就用p开p关
又来个章节 sis
引入文字 ion.
Firstly, the product is designed for oral use and our target user is the elderly. 英文分项不需要ol或ul
- The produc
- The short pep
- The expression level of short peptides has a threshold, preventing any excessive calcium absorption that might lead to hypercalcemia 注意句子超长后的回折
- There is a kill swit.
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Compared w 章节内分段 ique advantages tien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste oporosis patienChina has an estimated 70 million osteoporosis pat tien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste oporosis patienChina has an estimated 70 million osteoporosis pat tien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste oporosis patienChina has an estimated 70 million osteoporosis pat.
| Header 1 这是igem hq的表格的写法 | Header 2 |
|---|---|
| Content A 1 | Content B 1 |
| Content A 2 | Content B 2 这是igem hq的表格的写法。表格都需要说明，之前之后都会有p来文字 |
| Name | Item Name | Item Price |
|---|---|---|
| Alvin | Eclair | $0.87 |
| Alan | Jellybean | $3.76 |
| Jonathan | Lollipop | $7.00 |
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From the us 章节内分段 etition.
再来例子 Market Analysis
文本在左侧一半，宽屏 According to the Chinese Dietetic Sociietary Nutrient Intakes of Chinese Residents", the calcium requirement for infants and young children increases with age, from 300mg/day to 800mg/day, and the calcium requirement for adolescents reaches 1000mg/day. The amof the required intake, with the small exception of some coastal and pastoral areas 图片在右侧一半
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|---|---|---|
| Content A 1 | Content B 1 | Content C 1 |
| 列融合 | Content C 2 这是igem hq的表格的写法。半幅或者2/3幅可以用。 行融合 就 用br了 |
|
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