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| − | <h1>Combine World with Bone health</h1> | + | <h1 tabIndex="1">Combine World with Bone health</h1> |
| | <div class="column full_size"> | | <div class="column full_size"> |
| − | <p>Integrated Human Practice connects world and our projects. Inspired by the bone disease of old people, our project centers on osteoporosis and calcium absorption to constitute Bone Appetite. We constantly improve our design and execution by the feedbacks of public engagement, to make sure our project is good and responsible for the old, the whole human, the world.</p> | + | <p>Integrated Human Practice connects the world and our project. Inspired by the bone disease of old people, our project centers on osteoporosis and calcium absorption to constitute <i>Bone Appetite</i>. We constantly improve our design and execution by the feedbacks of public engagement, to make sure our project is good and responsible for the old, the whole human, the world. We compete for both the Silver Medal criterion #3 and the Best Integrated HP prize with this page.</p> |
| | </div> | | </div> |
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| | <div class="clear extra_space"></div><!-- 一个章节结束需要clear来留白 如果觉得内容间差异大再加上extra_space --> | | <div class="clear extra_space"></div><!-- 一个章节结束需要clear来留白 如果觉得内容间差异大再加上extra_space --> |
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| − | <h2>Frame and Overview</h2>
| + | <h2>Outline and Overview</h2> |
| − | <div class="column two_thirds_size"> | + | <div class="column full_size"> |
| − | <img src="https://static.igem.org/mediawiki/2020/8/8b/T--Fudan--map_2.svg" alt="map of calcium intake" /> | + | <img src="https://static.igem.org/mediawiki/2020/5/58/T--Fudan--Frame.svg" alt="Integration of Human Practice and Bone Appetide Project" /> |
| | + | <h6>Figure 1. Integration of Human Practice and Bone Appetide project</h6> |
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| | + | <p>Social surveys and Public engagement are the core of our Integrated Human Practices. When we gained our inspiration for bone health, we turned to social surveys and the critical stakeholders for the priority. Besides, picture and design our whole project according to their feedbacks.</p> |
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| | </div> | | </div> |
| − | <div class="column third_size">
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| − | <p>在宽屏，这部分内容占页面1/3；窄屏就是全幅。添加了column同时 图2/3 + 字1/3 正好宽屏的一幅。 China has an estimated 70 million osteo
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| − | porosis patien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste
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| − | oporosis patienChina has an estimated 70 million osteoporosis patien</p>
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| − | </div>
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| − | <div class="clear"></div><!-- 一个章节结束需要clear来留白 -->
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| | + | <div class="clear extra_space"></div><!-- 一个章节结束需要clear来留白 --> |
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| | <h2>Social Surveys</h2> | | <h2>Social Surveys</h2> |
| − | <div class="column two_thirds_size"> | + | <div class="column full_size"> |
| − | <p>Considering the COVID-19 pandemic, our team specially design an online questionnaire to do relevant social surveys. Due to our care about bone health and osteoporosis, we used widely social study to investigate public awareness and dig the potential need. For the old who has a hard availability of smartphone and the Internet, we visit the elderly community and senior home under the premise of keeping enough social distance and well protective measures. </p> | + | <p>Considering the COVID-19 pandemic, our team specially design an online questionnaire to do relevant social surveys. Due to our care about bone health and osteoporosis, we used widely social study to investigate public awareness and dig the potential need. For the old who has a hard availability of smartphone and the Internet, we visit the elderly community and senior home under the premise of keeping enough social distance and well protective measures. </p> |
| − | </div> | + | <img src="https://static.igem.org/mediawiki/2020/a/a9/T--Fudan--questionnairess.png" alt="The questionnaire of social surveys" /> |
| | + | <h6>Figure 2. The questionnaire of social surveys</h6></div> |
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| − | | + | <div class= "column half_size"> |
| − | <div class="clear"></div><!-- 一个章节结束需要clear来留白 --> | + | |
| − | <div class="column two_thirds_size"> | + | |
| | <h4>Public general knowledge</h4> | | <h4>Public general knowledge</h4> |
| | + | <p>The online surveys mainly direct at the people under 60 years old. We analyzed their knowledge of osteoporosis and daily calcium supplement situation. The result showed that the public has a strong awareness of osteoporosis and bone health along with the growing of age. For respondents aged 40 to 60, more than half of them are taking or have taken calcium supplements health care products. </p></div> |
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| − | <p>The online surveys mainly direct at the people under 60 years old. We analyzed their knowledge of osteoporosis and daily calcium supplement situation. The result showed that the public has a strong awareness of bone health along with the growing of age.</p></div> | + | <div class="column half_size"> <br/><br/><br/> |
| | + | <img src="https://static.igem.org/mediawiki/2020/9/95/T--Fudan--hp_chart3.png" alt="Calcium supplements situation of respondents aged 40 to 60" /> |
| | + | <h6>Figure 3. Calcium supplements situation of respondents aged 40 to 60</h6></div> |
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| − | <div class="clear"></div><!-- 一个章节结束需要clear来留白 --> | + | <div class="column half_size"><img src="https://static.igem.org/mediawiki/2020/1/1b/T--Fudan--hp_chart1.png" alt="Surrounding osteoporosis knowledge with different ages"/> |
| | + | <h6>Figure 4a. Surrounding osteoporosis knowledge with different ages</h6></div> |
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| − | | + | <div class="column half_size"><img src="https://static.igem.org/mediawiki/2020/ e/ e3/T--Fudan-- hp_chart2.png" alt="Surrounding osteoporosis knowledge with different ages"/> |
| − | <h4>The old people interviews</h4> | + | <h6>Figure 4b. Surrounding osteoporosis knowledge with different ages</h6> |
| − | <div class="column two_thirds_size"> | + | |
| − | <p>We believe the best suitable way to know the awareness message from the old is talking face to face. Connected with the largest student public-service association of Fudan University, we cooperated with “The Home of elderly” project, which aims at satisfy the need of the old. We interviewed dozens of aged people over 60 years old in a Shanghai elderly community. Then we were surprised by their extremely low awareness of osteoporosis and lack of calcium supplement.</p> </div>
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| − | <div class="column third_size">
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| − | <img src="https://static.igem.org/mediawiki/2020/ 3/ 37/T--Fudan-- upquestionnarie.png" /> | + | |
| | </div> | | </div> |
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| − | <div class="clear"></div><!-- 一个章节结束需要clear来留白 --> | + | <div class="column half_size"> |
| | + | <h4>Interviews with old people</h4> |
| | + | <p>We believe the best suitable way to know the awareness message from the old is talking face to face. Connected with the largest student public-service association of Fudan University, we cooperated with “The Home of Elderly” project, which aims at satisfy the need of the old. We interviewed dozens of aged people over 60 years old in a Shanghai elderly community. Then we were surprised by their extremely low awareness of osteoporosis and lack of calcium supplement.</p> </div> |
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| − | <div class="highlight decoration_background decoration_A_top"> | + | <div class=" column half_size"> |
| − | <p> | + | <br/><br/><br/> |
| − | The series of effective surveys provide us the reality of public awareness of calcium supplements and reveal the elderly’s problem. The evidence from the statistics analysis helped us to unearth the education need of the elderly. </p></div>
| + | <img src="https://static.igem.org/mediawiki/2020/1/1b/T--Fudan--oldcommunity.jpg" style="width:90%" alt="Interview the old in community"/><h6>Figure 5. Our teammate interview the old in the community</h6></div> |
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| | + | <div class="clear"></div><!-- 一个章节结束需要clear来留白 --> |
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| | + | <div class="highlight decoration_background decoration_B_top"> |
| | + | <p id="Integrated"> |
| | + | The series of effective surveys provide us the reality of public awareness of calcium supplements and reveal the elderly’s problem. The evidence from the statistics analysis helped us to unearth the education need of the elderly. <b>Specially, we came up with an idea of educating the younger generation and instructing them to educate their grandparents.</b></p> |
| | + | <p>Bone health is a serious problem for the old. They have a severe need for Calcium supplements. <b>We wanted to know the current treatment of osteoporosis and everyday health care products for Calcium supplement in this case.</b> We carried our thought to interview several key stakeholders and discuss the method to achieve them. Given the outbreak of the COVID-19 pandemic, we took action mainly in online form.</p></div> |
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| | <div class="clear extra_space"></div> | | <div class="clear extra_space"></div> |
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| | <h2>Online Public Engagement</h2> | | <h2>Online Public Engagement</h2> |
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| − | <div class="column full_size"> | + | <div class="column two_thirds_size"> |
| − | <h4>Various Stakeholders</h4>
| + | <h4>Dr. Dongying Qiu and Weiying Ren</h4> |
| − | <p><b>1.Dr.Dongying Qiu & Dr.Weidong Ren.</b></br>
| + | Deputy Chief Physician of Geriatrics Department, Zhongshan Hospital, Fudan University. |
| − | Deputy chief physician of Geriatrics Department of, Zhongshan Hospital, Fudan University. </p> | + | |
| − | <p>We consulted clinical physicians about the current osteoporosis treatment situation and FRAX (WHO Fracture Risk Assessment Tool), which is considered underestimating the Chinese population’s risk. They provided us plenty of important messages. First, the consultation rate of osteoporosis was quite low, owing to the old’s low awareness. Second, different assessment tools match various application scope. Guidelines for the diagnosis and management of primary osteoporosis provided by the Chinese Society of Osteoporosis and Bone Mineral Research are used in the clinic but still has question and limitation. What’s more, we exhibited our project design and education for older people to them, which gained great admiration. Dr.Qiu shared his own educational experience to instruct us. He suggested we should be careful with our expression, which needs to be scientific.</p> | + | <p>We consulted clinical physicians about the current osteoporosis treatment situation and FRAX (WHO Fracture Risk Assessment Tool), which is considered underestimating the Chinese population’s risk. They provided us plenty of important messages. </p> |
| | + | <p>First, the consultation rate of osteoporosis was quite low, owing to the old’s low awareness. </p> |
| | + | <p>Second, different assessment tools match various application scope. Guidelines for the diagnosis and management of primary osteoporosis provided by the Chinese Society of Osteoporosis and Bone Mineral Research are used in the clinic but still has question and limitation.</p> |
| | + | <p>What’s more, we exhibited our project design and education for older people to them, which gained great admiration. Dr.Qiu shared his own educational experience to instruct us. He suggested we should be careful with our expression, which needs to be scientific. |
| | + | </p></div> |
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| | + | <div class="column third_size"> |
| | + | <img src="https://static.igem.org/mediawiki/2020/7/7e/T--Fudan--Dr.Dongying_Qiu.jpg" alt="qiu dongying" style="width:38%" /> |
| | + | <img src="https://static.igem.org/mediawiki/2020/6/69/T--Fudan--Dr.Weiying_Ren.jpg" alt="ren weiying" style="width:45%" /></div> |
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| | + | <div class="column two_thirds_size"> |
| | + | <h4>Dr. Wei Hong</h4> |
| | + | Deputy Chief Physician of Osteoporosis Department, East China Hospital affiliated to Fudan University |
| | | | |
| | + | <p>Dr. Hong is a professor in the area of osteoporosis who also makes an effort to educate patients. |
| | + | We discussed the design of the assessment tool and how to improve the current treatment and recovery method. Dr.Hong pointed out the self-assessment tool's difficulty because of the lack of a database that big enough. We learned that the community is the most effective way to carry out education and recovery introduction. The direct contact with the old helps continuous supervision of treatment progress. |
| | + | </p></div> |
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| − | <p><b>2.Dr.Dong Wei</b></br> | + | <div class="column third_size"> |
| − | Deputy chief physician of Osteoporosis Department, East China Hospital affiliated to Fudan University </p>
| + | <img src="https://static.igem.org/mediawiki/2020/4/4b/T--Fudan--Dr.Wei_Hong.jpg" alt="wei hong" style="width:60%"/></div> |
| − | <p>Dr.Hong is a professor in the area of osteoporosis who also makes an effort to educate patients. | + | |
| − | We discussed the design of the assessment tool and how to improve the current treatment and recovery method. Dr. Hong pointed out the self-assessment tool’s difficulty because of the lack of a database that big enough. We learned that the community is the most effective way to carry out education and recovery introduction. The direct contact with the old helps continuous supervision of treatment progress.
| + | <div class="clear"></div> |
| − | </p> | + | |
| | + | <div class="highlight decoration_background decoration_B_top"> |
| | + | <p>The pool assessment tool and low osteoporosis rate reminded us of the difficulty for the old's bone health. To better guarantee more comprehensive bone health, we tried to achieve an effective Calcium supplement method. We analyzed the current way and relevant health care products market. We came up with some questions and existed difficulty: </p><ol> |
| | + | <li>Patients usually forget to take supplements, especially for the old. </li> |
| | + | <li>Calcium absorption rate drops as the amount of calcium intake increases.</li> </ol> |
| | + | <p>Current market is in urgent need of a supplement method that ensures a high calcium absorption rate over a long period of time. This will avoid frequent intake of a large amount of supplement pills and the precipitation of calcium oxalate. We decided to <a href="/Team:Fudan/Design">design</a> a <b>high and sustainable calcium absorption nutrition</b>. </p></div> |
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| | + | <div class="clear"></div> |
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| | + | <div class="column two_thirds_size"> |
| | + | <h4>Senior home staff and care workers</h4> |
| | + | Xinguang Senior Home, Shanghai |
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| − | <p><b>3.Senior home staff and care workers</b></br>
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| − | Xinguang senior home </p>
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| | <p>In Xinguang senior home, they have nearly 100 elders aged 60 to 100. We interviewed their staff about the bone health of the old. They reflected several severe fractures caused by osteoporosis and pointed their general bad bone health. | | <p>In Xinguang senior home, they have nearly 100 elders aged 60 to 100. We interviewed their staff about the bone health of the old. They reflected several severe fractures caused by osteoporosis and pointed their general bad bone health. |
| − | </p> | + | </p></div> |
| − | </div>
| + | <div class="column third_size"> |
| | + | <img src="https://static.igem.org/mediawiki/2020/7/7a/T--Fudan--seniorhome.jpg" alt="senior home" style="width:80%"/></div> |
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| − | <div class="column full_size"><!-- 全幅面的文字内容，上面是否留白可选 -->
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| − | <p>However, for the Chinese population, the bottlenecks are
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| − | as follows:
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| − | </p>
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| − | <ol>
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| − | <li>注意这里列表的写法 页面字会变深 突出内容 ol是编数字的 nts</li>
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| − | <li>calcium-supplementing pills daste</li>
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| − | <li>The body's absorptioes as calcium intake increases</li>
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| − | <li>Vegetables conta列表的写法 页面字会变深 突出内容 ol是编数字的 ul是没有标数字的列表 </li>
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| − | </ol>
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| − | <p>
| + | |
| − | However, the currently popular calcium supplement method on the market is either to adjust diet or to consume a lot of calcium supplementing pills. For the elderly, they suffer from poor memory or neglect the importance of calcium pills, often forgetting to consume the supplement; for children, they often refuse to take calcium pills or to maintain some diet high in calcium, thus requiring the "liquid calcium" method to ensure sufficient calcium intake. Ho可以 继续大段内容
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| − | </p></div>
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| − | <div class="clear extra_space"></div ><!-- 一个章节结束需要clear来留白 --> | + | |
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| | + | <div class="column two_thirds_size"> |
| | + | <h4>Prof. Jinzhong Lin</h4> |
| | + | School of Life Sciences Fudan University |
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| − | <h2>Collaboration</h2>
| + | <p>We discussed with Professor Lin, who is engaged in prokaryotic expression work at the Fudan University Academy of Sciences. The concept of quorum sensing system described was proposed by Fudan 2019. To help engineered bacteria be competitive enough in the intestine, MccB17 needed better expressed. We thought about using the high-copy plasmids to express MccB17, but they are more challenging to regulate and respond sensitively and extensively to increase engineered bacteria's expression burden.<br/> |
| − | <div class="column third_size"> | + | </div> |
| − | <img src="https://static.igem.org/mediawiki/2020/8/ 8b/T--Fudan-- map_2. svg" alt=" map of calcium intake" /> | + | <div class="column third_size"><br/><br/> |
| − | </div>
| + | <img src="https://static.igem.org/mediawiki/2020/8/84/T--Fudan--jinzhonglin.png" alt="lin jinzhong" style="width:50%"/></div> |
| − | <div class="column two_thirds_size">
| + | |
| − | <p>We collaborate with team TJU held an online summer camp for high school students. The activity lasted for two days, and a total of nearly 40 students ranging from grades 9 to 12 participated in it. In the event, we introduced to them the relevant principles and concepts of synthetic biology and shared the concept of iGEM as well as our project. </br>
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| − | This year team SCU-China's program is related to fermentation production. They plan to show the different fermentation processes of traditional flour-made food from all over China. Similar to our project, we both focus on nutrition and diet. Some members of our team provided SCU-China with photographs and instructions of flour specialties in their hometown.</br>
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| − | <a href="https://2020.igem.org/Team:Fudan/Collaboration"> Click here to see more details</a></p>
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| − | </div>
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| − | <div class="column full_size">
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| − | <h4>Model</h4>
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| − | <p>We establish two models: quorum sensing process and Kill Switch. We refer to the model and data of 2019Fudan team and use the random process to describe it more clearly to model the quorum sensing process in stages and levels. In the kill switch section, we use the 19BNU team’s data, improve the logistic equation, adjust the promoter properties, and make a sensitivity analysis. We can see good results through it. </p>
| + | |
| − | <p>In the quorum sensing part, we used the Gillespie algorithm in random simulation to simulate the macro and micro levels’ physiological processes. It expresses luxI and luxR genes at the micro-level and produces dimers before the population reaches a certain size. At the macro level, Escherichia coli keeps proliferating, excreting antimicrobial peptides, and colonizing the intestinal tract during this process. When the population number is sufficiently large, the short calc-absorbing peptide and the regulation system begin to express. In contrast, the antimicrobial peptide is expressed but cannot be discharged so that the bacterial number can achieve dynamic stability.
| + | |
| − | </p>
| + | |
| − | <h4>Kill switch</h4>
| + | |
| − | <p>We contacted Mr. Feng in BNU, a member of 19BNU-China and very experienced in kill switches, discussing the kill switch and safety issue of antibiotics resistance gene design. </p>
| + | |
| | | | |
| − | <p>We doubted whether the glucose-starvation promotor (BBa_K3036003 from 19BNU-China) would be a suitable response system for our kill switch and hope to obtain their plasmids as a control group directly to save time for constructing it from the beginning. | + | <div class="column full_size"><div class="highlight decoration_background decoration_A_top"> |
| − | Mr. Feng didn’t consider glucose concentration as a distinct signal to trigger glucose response that existed both inside and outside the body. The difference between the two conditions was not very significant. And he also recommended we search some literature on toxin/antitoxin systems. They probably were ideal candidates for carrying eliminating function.</p>
| + | <p>We designed a peptide (CaAP) containing a series of short peptides that can promote calcium absorption. We let probiotics express and secrete this peptide in the intestine to promote calcium absorption in the intestine.</p> |
| − | <p>After search the advantages of the toxin/antitoxin system, we decided to undergo an overhaul of our kill switch. This consultation helped us summarize the idea of designing a kill switch that kill switch circuit can be divided into two core components: judging whether bacteria leave the set condition and the other component to kill the bacteria when it leaves the set condition. </p> | + | |
| − | <p>Antibiotics resistance genes encoded on a plasmid can transfer to the host’s intestinal flora and hurries the development of antibiotic-resistant strains. To improve our product’s safety factor, we wanted to insert our genetic circuits into the genome to inhibit the horizontal transfer. Mr. Feng told us that verifying genome insertion in experiments would take a significant amount of time and was unlikely to be done within a few months. Using plasmids in the experimental stage is still a preferable way. He commended that multi-copy insertion could be designed as the final form of the product.
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| − | </p> </div> | + | |
| − | <div class="clear"></div><!-- 一个章节结束需要clear来留白 -->
| + | |
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| | + | <p>Prof. Lin suggested us to use low-copy plasmids to reduce the expression burden of engineered bacteria. We tried to keep the main part of MccB17 (McbABCD) a relatively low expression and high express the immune part (McbEFG) to achieve high secretion of MccB17.</p></div></div> |
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| − | <h2>Education</h2> | + | <div class="clear extra_space"></div><!-- 一个章节结束需要clear来留白 --> |
| | + | |
| | + | <h2>Collaboration</h2> |
| | <div class="column third_size"> | | <div class="column third_size"> |
| − | <img src="https://static.igem.org/mediawiki/2020/ 8/ 8b/T--Fudan-- map_2. svg" alt=" map of calcium intake" /> | + | <img src="https://static.igem.org/mediawiki/2020/9/93/T--Fudan--TJU.jpg" alt="TJU" /></div> |
| | + | <div class="column third_size"> |
| | + | <img src = "https://static.igem.org/mediawiki/2020/a/a6/T--Fudan--SCU.jpg" alt="SCU" /> |
| | </div> | | </div> |
| − | <div class="column two_thirds_size">
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| − | <p><b>Educate the old through younger generation.</b></p>
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| − | <p>Educating the elderly helps the old keep health and protects them from fraud and mis-information. We used a previous unexplored education approach to educate biologic knowledge to the old. Our main strategy is educating the younger generation and instructing them how to educate their grandparents. Compared with traditional education strategies, our approach offers emotional support from the younger generation and is comfortable for the old. In countries like Japan, Korea, Singapore and China, where the bond between grandparents and grandkids is incredibly tight, our approach is undoubtedly effective. </br>
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| − | To educate the youth, we have held online summer camps for high school students to pass on aged care, in addition to synthetic biology which we have been teaching in the past several years. We developed an online audio course on basic biology knowledge, which could been listened by both the youth and the old. </p>
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| − | <p>In addition, we combined osteoporosis prevention with traditional Chinese art Xiangsheng, and taught it to the old living in a nursing home (we self as the youth). Also, we wrote Xiàng Sheng (a popular traditional art in China) and other outreach materials for people to use. In summary, we made brave and compelling attempts to implement science communication between the younger generation and the long-neglected social communities - the old.</p>
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| − | <p><a href="https://2020.igem.org/Team:Fudan/Education">Click here to see more details</a></p>
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| − | </div>
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| − | <h2> Commercialization analysis</h2>
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| | <div class="column third_size"> | | <div class="column third_size"> |
| − | <img src="https://static.igem.org/mediawiki/2020/8/8b/T--Fudan--map_2.svg" alt="map of calcium intake" /> | + | <p>We collaborate with team TJU held an online summer camp for high school students to introduced the relevant principles and concepts of synthetic biology and shared the concept of iGEM as well as our project. More details at |
| | + | <a href="/Team:Fudan/Collaborations">/Team:Fudan/Collaborations</a>.</p> |
| | </div> | | </div> |
| − | <div class="column two_thirds_size">
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| − | <p> We made the market investigations and pitched our commercial plan to potential commercial investors. During these interactions, we improved our existing design for CaAP, and we came up with the CaAss test strip product, which later got support by our pilot experiments.</p>
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| − | <p> <a href="https://2020.igem.org/Team:Fudan/Entrepreneurship"> Click here to see more details</a></p></div>
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| − | <div class="clear"></div>
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| − | <div class=" column full_size"> | + | <div class="clear"></div> |
| − | <p>
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| − | <b>1.Commercial consult.</b> We contacted factories about our product's cost, more specifically, about the cost of producing pills from lab benches to packages ready for the market.We scheduled several in-person and online appointments with professional investors in the biochemical industry and corresponded with them back and forth to polish our business plan. We also obtained a letter of intent from them, paving ways for future collaboration. Besides, we asked for advice from peers in the Fudan school of management about our marketing strategies and shaped our business plan to target our potential users more effectively.</p>
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| − | < p>< b> 2.CaAass developing.</ b> To obtain more professional feedback and get our projects wider public known, we participated in the Fanhai Bei innovation competition and won a third-class prize. According the judges' feedback, we modified our business model and found that older people in China lacked an easy method to assess their calcium status. Therefore, we developed our Measurement project CaAss to provide a solution for the old people to measure their blood calcium status in a portable way. <a href=" https://2020.igem.org/Team:Fudan/ Measurement"> Click here to see more details</a></p> | + | <div class="column full_size"> |
| | + | <h4>Model</h4> |
| | + | <p>We establish two models: quorum sensing process and Kill Switch. We refer to the model and data of 2019Fudan team and use the random process to describe it more clearly to model the quorum sensing process in stages and levels. In the kill switch section, we use the 19BNU team’s data, improve the logistic equation, adjust the promoter properties, and make a sensitivity analysis. We can see good results through it. |
| | + | More details at |
| | + | <a href="/Team:Fudan/Model#killswitch">/Team:Fudan/Model</a>.</p></div> |
| | | | |
| | + | <div class = "clear"></div> |
| | | | |
| − | </div> | + | <div class="column full_size"> |
| | + | <h4>Kill Switch design</h4> |
| | + | <p>We contacted Mr. Feng in BNU, a member of 19BNU-China and very experienced in kill switches, discussing the kill switch and safety issue of antibiotics resistance gene design. </p></div> |
| | + | <div class = "column two_thirds_size"> |
| | + | <img src="https://static.igem.org/mediawiki/2020/2/26/T--Fudan--BNU2.jpg" alt="BNU2" /></div> |
| | + | <div class = "column third_size"> |
| | + | <p>We discussed whether the glucose-starvation promotor (BBa_K3036003 from 19BNU-China) would be a suitable response system for our kill switch and hope to obtain their plasmids as a control group directly to save time for constructing it from the beginning. |
| | + | Mr. Feng didn’t consider glucose concentration as a distinct signal to trigger glucose response that existed both inside and outside the body. The difference between the two conditions was not very significant. And he also recommended we search some literature on toxin/antitoxin systems. They probably were ideal candidates for carrying eliminating function.</p></div> |
| | | | |
| − | <div class="clear"></div ><!-- 一个章节结束需要clear来留白 --> | + | <div class = "clear"></div> |
| | | | |
| − | <div class="column full_size" ><!-- 全幅面的文字内容，上面是否留白可选 --> | + | <div class = "column full_size"> |
| − | <p> However, for the Chinese population, the bottlenecks are | + | <p>After search the advantages of the toxin/antitoxin system, we decided to undergo an overhaul of our kill switch. This consultation helped us summarize the idea of designing a kill switch that kill switch circuit can be divided into two core components: judging whether bacteria leave the set condition and the other component to kill the bacteria when it leaves the set condition. </p> |
| − | as follows:
| + | |
| − | </p>
| + | |
| − | <ol>
| + | |
| − | <li>注意这里列表的写法 页面字会变深 突出内容 ol是编数字的 nts</li>
| + | |
| − | <li>calcium-supplementing pills daste</li>
| + | |
| − | <li>The body's absorptioes as calcium intake increases</li>
| + | |
| − | <li>Vegetables conta列表的写法 页面字会变深 突出内容 ol是编数字的 ul是没有标数字的列表 </li>
| + | |
| − | </ol>
| + | |
| − | <p>
| + | |
| − | However, the currently popular calcium supplement method on the market is either to adjust diet or to consume a lot of calcium supplementing pills. For the elderly, they suffer from poor memory or neglect the importance of calcium pills, often forgetting to consume the supplement; for children, they often refuse to take calcium pills or to maintain some diet high in calcium, thus requiring the "liquid calcium" method to ensure sufficient calcium intake. Ho可以 继续大段内容
| + | |
| − | </p>
| + | |
| | | | |
| | + | <p>Antibiotics resistance genes encoded on a plasmid can transfer to the host’s intestinal flora and hurries the development of antibiotic-resistant strains. To improve our product’s safety factor, we wanted to insert our genetic circuits into the genome to inhibit the horizontal transfer. Mr. Feng told us that verifying genome insertion in experiments would take a significant amount of time and was unlikely to be done within a few months. Using plasmids in the experimental stage is still a preferable way. He commended that multi-copy insertion could be designed as the final form of the product. |
| | + | </p> </div> |
| | | | |
| − | <div class="highlight decoration_background decoration_A_top">
| + | <div class="clear extra_space"></div><!-- 一个章节结束需要clear来留白 --> |
| − | <p> | + | |
| − | 章节内容的分段就用p开p关，额外需要关注的可以套一个highlight的框，有多种版本
| + | |
| − | </p>
| + | |
| − | </div>
| + | |
| − | <p>
| + | |
| − | 章节内容的分段就用p开p关
| + | |
| − | </p>
| + | |
| − | <div class="highlight decoration_B_full"> | + | |
| − | <p>
| + | |
| − | 章节内容的分段就用p开p关，额外需要关注的可以套一个highlight的框，细看 https://2020.igem.org/Resources/Template_Documentation#highlight
| + | |
| − | </p>
| + | |
| − | </div>
| + | |
| − | <p>
| + | |
| − | 章节内容的分段就用p开p关
| + | |
| − | </p>
| + | |
| − | <p>
| + | |
| − | 章节内容的分段就用p开p关 <a href="https://2020.igem.org/Team:Fudan/Entrepreneurship"> https://2020.igem.org/Team:Fudan/Entrepreneurship</a>
| + | |
| − | </p>
| + | |
| − | <ul>
| + | |
| − | <li>注意这里列表的写法 页面字会变深 突出内容 ul是没有标数字的列表 nts</li>
| + | |
| − | <li>calcium-supple menting pills don’t haat taste</li>
| + | |
| − | <li>Vegetables conta列表的写法 页面字会变深 突出内容 ol是编数字的 ul是没有标数字的列表 </li>
| + | |
| − | </ul>
| + | |
| − | <p>
| + | |
| − | 章节内容的分段就用p开p关
| + | |
| − | </p>
| + | |
| − | </div><!-- 每个幅面结束都需要 关div -->
| + | |
| | | | |
| − | <div class="clear extra_space"></div><!-- 一个章节结束需要clear来留白 如果觉得内容间差异大再加上extra_space --> | + | <h2>Education</h2> |
| | | | |
| − | <h2>又来个章节 sis</h2>
| |
| | <div class="column full_size"> | | <div class="column full_size"> |
| − | <p>引入文字 ion.</p>
| + | <h4>Educate the old through younger generation.</h4> |
| − | <p> Firstly, the product is designed for oral use and our target user is the elderly. 英文分项不需要ol或ul</p> | + | <p>Educating the elderly helps the old keep health and protects them from fraud and mis-information. We used a previous unexplored education approach to educate biologic knowledge to the old. Our main strategy is educating the younger generation and instructing them how to educate their grandparents. Compared with traditional education strategies, our approach offers emotional support from the younger generation and is comfortable for the old.In addition, we combined osteoporosis prevention with traditional Chinese art Crosstalk, and taught it to the old living in a nursing home. In summary, we made brave and compelling attempts to implement science communication between the younger generation and the long-neglected social communities - the old.</p> |
| − | <ol>
| + | <p>To educate the youth, we held online summer camps for high school students to pass on aged care, in addition to synthetic biology which we have been teaching in the past several years. We developed an online audio course on basic biology knowledge, which could been listened by both the youth and the old. </p> |
| − | <li>The produc</li>
| + | |
| − | <li>The short pep</li>
| + | |
| − | <li>The expression level of short peptides has a threshold, preventing any excessive calcium absorption that might lead to hypercalcemia 注意句子超长后的回折</li>
| + | |
| − | <li>There is a kill swit.</li>
| + | |
| − | </ol>
| + | |
| | | | |
| − | | + | <p>More details at <a href="/Team:Fudan/Education">/Team:Fudan/Education</a>.</p> |
| − | Based on relevant research, the product is exp 章节内分段 ina. tien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste
| + | </div> |
| − | oporosis patienChina has an estimated 70 million osteoporosis pat
| + | |
| − | </p>
| + | |
| | | | |
| − | <p>Compared w 章节内分段 ique advantages tien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste
| + | <div class="clear extra_space"></div><!-- 一个章节结束需要clear来留白 --> |
| − | oporosis patienChina has an estimated 70 million osteoporosis pat tien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste
| + | |
| − | oporosis patienChina has an estimated 70 million osteoporosis pat tien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste
| + | |
| − | oporosis patienChina has an estimated 70 million osteoporosis pat.
| + | |
| − | </p>
| + | |
| | | | |
| − | <table> | + | <h2>Commercialization analysis</h2> |
| − | <tr>
| + | <div class="column two_thirds_size"> |
| − | <th> Header 1 这是igem hq的表格的写法 </th> <th> Header 2 </th>
| + | <img src="https://static.igem.org/mediawiki/2020/b/b4/T--Fudan--timeline.jpg" alt="commercialization timeline" /> |
| − | </tr> | + | |
| − | <tr> | + | |
| − | <td> Content A 1 </td> <td> Content B 1 </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td> Content A 2 </td> <td> Content B 2 这是igem hq的表格的写法。表格都需要说明，之前之后都会有p来文字 </td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | | + | |
| − | <p></p><!-- 无文字p是换行隔开，在关闭的html元素间回车是没有用的 -->
| + | |
| − | | + | |
| − | <table class="striped"><!-- 这是另一个版本的表格，间隔行有阴影。一个页面不建议交叉两种格式。太宽表格可以加 responsive-table 方便窄屏 -->
| + | |
| − | <tr>
| + | |
| − | <th>Name</th> <th>Item Name</th> <th>Item Price</th>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Alvin</td> <td>Eclair</td> <td>$0.87</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Alan</td> <td>Jellybean</td> <td>$3.76</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Jonathan</td> <td>Lollipop</td> <td>$7.00</td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | | + | |
| − | <p>Calciu 章节内分段 fety. tien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste
| + | |
| − | oporosis patienChina has an estima
| + | |
| − | ted 70 million osteoporosis pat tien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste
| + | |
| − | oporosis patienChina has an estimated 70 mill
| + | |
| − | ion osteoporosis pattien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste
| + | |
| − | oporosis patienChina has an estimated 70 million osteoporosis pat
| + | |
| − | </p>
| + | |
| − | | + | |
| − | <p>From the us 章节内分段 etition.</p>
| + | |
| | </div> | | </div> |
| | + | <div class="column third_size"> |
| | + | <p> We made the market investigations and pitched our commercial plan to potential commercial investors. During these interactions, we improved our existing design for CaAP, and we came up with the CaAsst test strip product, which later got support by our pilot experiments. More details at <a href="/Team:Fudan/Entrepreneurship"> /Team:Fudan/Entrepreneurship</a>.</p></div> |
| | | | |
| − | <div class="clear"></div ><!-- 一个章节结束需要clear来留白 --> | + | <div class="clear"></div> |
| | | | |
| − | <h2>再来例子 Market Analysis</h2>
| + | <div class="column full_size"> |
| − | <div class="column half_size"> | + | <p><b>1. Commercial consult.</b> We contacted factories about our product's cost, more specifically, about the cost of producing pills from lab benches to packages ready for the market. Wescheduled several in-person and online appointments with professional investors in the biochemical industry and corresponded with them back and forth to polish our business plan. We also obtained a letter of intent from them, paving ways for future collaboration. Besides, we asked for advice from peers in the Fudan school of management about our marketing strategies and shaped our business plan to target our potential users more effectively.</p> |
| − | <p> 文本在左侧一半，宽屏 According to the Chinese Dietetic Sociietary Nutrient Intakes of Chinese Residents", the calcium requirement for infants and young children increases with age, from 300mg/day to 800mg/day, and the calcium requirement for adolescents reaches 1000mg/day. The amof the required intake, with the small exception of some coastal and pastoral areas 图片在右侧一半</p> | + | |
| | | | |
| − | <table> | + | <p><b>2. CaAsst developing.</b> To obtain more professional feedback and get our projects wider public known, we participated in the Fanhai Bei innovation competition and won a third-class prize. According the judges' feedback, we modified our business model and found that older people in China lacked an easy method to assess their calcium status. Therefore, we developed our Measurement project CaAsst to provide a solution for the old people to measure their blood calcium status in a portable way. More details at <a href="/Team:Fudan/Measurement">/Team:Fudan/Measurement</a></p></div> |
| − | <tr> | + | |
| − | <th> 这是igem hq的表格的写法 </th> <th> Header 2 </th> <th> Header 3 </th>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td> Content A 1 </td> <td> Content B 1 </td> <td> Content C 1 </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td colspan=2> 列融合 </td> <td> Content C 2 这是igem hq的表格的写法。半幅或者2/3幅可以用。<br/>行融合<br/>就<br/>用br了 </td>
| + | |
| − | </tr> | + | |
| − | </table> | + | |
| | | | |
| − | </div>
| + | <div class="clear extra_space"></div><!-- 一个章节结束需要clear来留白 因为马上最后一幅，建议extra_space --> |
| − | <div class=" column half_size"> | + | |
| − | <img src="https://static.igem.org/mediawiki/2020/0/00/T--Fudan--calcium_intake_graph.svg" alt="calcium intake graph">
| + | |
| − | </div>
| + | |
| | | | |
| − | <div class="clear extra_space"></div><!-- 一个章节结束需要clear来留白 因为马上最后一幅，建议extra_space -->
| + | <h2>Summary</h2> |
| | | | |
| − | <div class="column full_size"> | + | <div class="column full_size"> |
| − | <p> 每页结束需要小结，可以 ul ol 列表highlight，可以是每页第一段的另一种写法fety. tien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste | + | <p>Our project focus on reducing the impact of osteoporosis, trying search the way to making world better. </p> |
| − | oporosis patienChina has an estima
| + | <div class="highlight decoration_background"> |
| − | ted 70 million osteoporosis pat tien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste
| + | <ol> |
| − | oporosis patienChina has an estimated 70 mi 每页结束需要小结，可以 ul ol 列表highlight，可以是每页第一段的另一种写法</p>
| + | <li> Analyzed the whole ages surveys to investigate public awareness and the current suffering situation of the old people.</li> |
| − | </div>
| + | <li> Interviewed various stakeholders, including clinicians, professors and care workers to gather the limits of current treatments and used these feedbacks to design our project. </li> |
| | + | <li> Connected with members of 19BNU and 19Fudan to gain their valuable insights and outline our wet lab experiments.</li> |
| | + | <li> Made the market investigations and pitched our commercial plan to potential commercial investors.</li> |
| | + | <li> In order to educate the aged people more effectively, we communicated with care workers and created instructional materials on care given.</li> |
| | + | </ol> |
| | + | </div> |
| | + | <p> During these interactions, we improved our existing design for CaAP, and we came up with the CaAsst test strip product, which later got support by our pilot experiments.Our team successfully integrated Human Practice to design and improve our Bone Appetite project. </p> |
| | + | </div> |
| | | | |
| | <div class="column full_size"><div id="FudanSignature"> | | <div class="column full_size"><div id="FudanSignature"> |
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| | <div class="container"><div class="row"> | | <div class="container"><div class="row"> |
| | <div class="col m3 s12 row" | | <div class="col m3 s12 row" |
| − | style="margin-top:4rem" > | + | style="margin-top:4rem" tabIndex="0" > |
| | <a href="http://www.yfc.cn/" target="_blank"><img class="col s3 m6 l4 FudanSponsors" alt="Yunfeng Capital" src="https://static.igem.org/mediawiki/2020/b/ba/T--Fudan--YFcapital.svg"> </a> | | <a href="http://www.yfc.cn/" target="_blank"><img class="col s3 m6 l4 FudanSponsors" alt="Yunfeng Capital" src="https://static.igem.org/mediawiki/2020/b/ba/T--Fudan--YFcapital.svg"> </a> |
| | <a href="http://www.fudan.edu.cn/en/" target="_blank"><img alt="Fudan University" class="col s3 m6 l4 FudanSponsors" src="https://static.igem.org/mediawiki/2020/9/9d/T--Fudan--sponsor2.svg"> </a> | | <a href="http://www.fudan.edu.cn/en/" target="_blank"><img alt="Fudan University" class="col s3 m6 l4 FudanSponsors" src="https://static.igem.org/mediawiki/2020/9/9d/T--Fudan--sponsor2.svg"> </a> |
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| | <div class="col s12 l6 row"> | | <div class="col s12 l6 row"> |
| | <div class="col s12 m4"> | | <div class="col s12 m4"> |
| − | <span><a href="/Team:Fudan/Description#Project">Project</a></span> | + | <span tabIndex="2"><a href="/Team:Fudan/Description#Project">Project</a></span> |
| | <ul><li><a href="/Team:Fudan/Description">Description</a></li> | | <ul><li><a href="/Team:Fudan/Description">Description</a></li> |
| | <li><a href="/Team:Fudan/Sustainable">Sustainable</a></li> | | <li><a href="/Team:Fudan/Sustainable">Sustainable</a></li> |
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| | </ul></div> | | </ul></div> |
| | <div class="col s12 m4"> | | <div class="col s12 m4"> |
| − | <span><a href="/Team:Fudan/Notebook">Notebook</a></span> | + | <span tabIndex="3"><a href="/Team:Fudan/Notebook">Notebook</a></span> |
| | <ul><li><a href="/Team:Fudan/Safety">Safety</a></li> | | <ul><li><a href="/Team:Fudan/Safety">Safety</a></li> |
| | <li><a href="/Team:Fudan/Poster">Poster</a></li> | | <li><a href="/Team:Fudan/Poster">Poster</a></li> |
| Line 552: |
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| | </ul></div> | | </ul></div> |
| | <div class="col s12 m4"> | | <div class="col s12 m4"> |
| − | <span><a href="/Team:Fudan/Results">Results</a></span> | + | <span tabIndex="4"><a href="/Team:Fudan/Results">Results</a></span> |
| | <ul><li><a href="/Team:Fudan/Contribution">Contribution</a></li><!-- replace Judging --> | | <ul><li><a href="/Team:Fudan/Contribution">Contribution</a></li><!-- replace Judging --> |
| | <li><a href="/Team:Fudan/Implementation">Implementation</a></li> | | <li><a href="/Team:Fudan/Implementation">Implementation</a></li> |
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| | <div class="col s12 l6 row"> | | <div class="col s12 l6 row"> |
| | <div class="col s12 m4"> | | <div class="col s12 m4"> |
| − | <span><a href="/Team:Fudan/Parts">Parts</a></span> | + | <span tabIndex="5"><a href="/Team:Fudan/Parts">Parts</a></span> |
| | <ul><li><a href="/Team:Fudan/Parts#Basic">Basic part</a></li> | | <ul><li><a href="/Team:Fudan/Parts#Basic">Basic part</a></li> |
| | <li><a href="/Team:Fudan/Parts#Composite">Composite part</a></li> | | <li><a href="/Team:Fudan/Parts#Composite">Composite part</a></li> |
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| | </ul></div> | | </ul></div> |
| | <div class="col s12 m4"> | | <div class="col s12 m4"> |
| − | <span><a href="/Team:Fudan/Human_Practices">Outreach</a></span> | + | <span tabIndex="6"><a href="/Team:Fudan/Human_Practices">Outreach</a></span> |
| | <ul><li><a href="/Team:Fudan/Human_Practices#Integrated">Integrated HP</a></li> | | <ul><li><a href="/Team:Fudan/Human_Practices#Integrated">Integrated HP</a></li> |
| | <li><a href="/Team:Fudan/Education">Education</a></li> | | <li><a href="/Team:Fudan/Education">Education</a></li> |
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| | </ul></div> | | </ul></div> |
| | <div class="col s12 m4"> | | <div class="col s12 m4"> |
| − | <span><a href="/Team:Fudan/Team">Team</a></span> | + | <span tabIndex="7"><a href="/Team:Fudan/Team">Team</a></span> |
| | <ul><li><a href="/Team:Fudan/Team">Members</a></li> | | <ul><li><a href="/Team:Fudan/Team">Members</a></li> |
| | <li><a href="/Team:Fudan/Attributions">Attributions</a></li> | | <li><a href="/Team:Fudan/Attributions">Attributions</a></li> |
| | <li><a href="/Team:Fudan/Partnership">Partnership</a></li> | | <li><a href="/Team:Fudan/Partnership">Partnership</a></li> |
| | <li><a href="/Team:Fudan/Acknowledgement">Acknowledgement</a></li> | | <li><a href="/Team:Fudan/Acknowledgement">Acknowledgement</a></li> |
| − | <li><a href="https://2019.igem.org/Team:Fudan-TSI/Heritage" target="_blank">Heritage</a></li> | + | <li><a href="/Team:Fudan/Heritage">Heritage</a></li> |
| | </ul></div> | | </ul></div> |
| | <div class="col s12 m4"> </div> | | <div class="col s12 m4"> </div> |
