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− | <h1>Safety</h1> | + | <h1 tabIndex="1">The most primary issue to consider</h1> |
| <div class="column full_size"> | | <div class="column full_size"> |
− | <p>Safety is always the most primary issue to consider, especially for projects of which potential consumers are the vulnerable elderly. The risks for everyone who has access to engineered probiotic from production to consumption should be carefully identified and managed. </p> | + | <p>Safety is always the most primary issue to consider, especially for projects in which potential consumers are the vulnerable elderly. The risks for everyone who has access to engineered probiotic from production to consumption should be carefully identified and managed.</p> |
| </div> | | </div> |
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− | <div class="clear extra_space"></div><!-- 一个章节结束需要clear来留白 如果觉得内容间差异大再加上extra_space --> | + | <div class="clear"></div><!-- 一个章节结束需要clear来留白 如果觉得内容间差异大再加上extra_space --> |
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| <h2>Laboratory Safety</h2> | | <h2>Laboratory Safety</h2> |
− | <div class="column two_thirds_size">
| + | <div class="column full_size" > |
− | <img src="https://static.igem.org/mediawiki/2020/8/8b/T--Fudan--map_2.svg" alt="map of calcium intake" /> | + | <p>The Fudan team is fully compliant with the safety and security policies of the iGEM. Safety has been carefully and thoroughly considered throughout our project design and experimental operation.</p></div> |
− | </div>
| + | |
− | <div class="column third_size">
| + | |
− | <p>The Fudan team is fully compliant with the safety and security policies of the iGEM. Safety has been carefully and thoroughly considered throughout our project design and experimental operation. | + | |
− | </div>
| + | |
− | <div class="clear extra_space"></div><!-- 一个章节结束需要clear来留白 如果觉得内容间差异大再加上extra_space -->
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− | <h4>Safety training</h4>
| + | |
− | <div class="column full_size" >
| + | |
− | <p>All members in our team have taken online courses in biosafety, such as hazardous chemicals, laboratory waste, special equipment safety, accident handling, and passed laboratory safety examination required by Assets and Security Department of our university before we entering the lab. </p>
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− | </div>
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| <div class="clear"></div><!-- 一个章节结束需要clear来留白 如果觉得内容间差异大再加上extra_space --> | | <div class="clear"></div><!-- 一个章节结束需要clear来留白 如果觉得内容间差异大再加上extra_space --> |
− | <h4>Lab work</h4>
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− | <div class="column full_size" >
| |
− | <p>Our experiments were all performed in the laboratory of Prof. Liang Cai in School of Life Sciences, which is qualified for Biosafety Level 2. For safety equipment, the lab has biosafety cabinets allowing us to carry out most experiments safely. There is a cabinet to store the hazardous reagents, the key of which only Prof. Cai has and have a dedicated refrigerator for iGEM to store our experimental materials including plasmids and primers. Fire extinguishers, fire hydrants, emergency spray and other safety facilities are also available in the lab to deal with different accidental situations.
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− | As the supervisor of lab, Prof. Cai also provided us with systematic safety guidelines and in field training before we started lab affair. Every time we conducted our experiments, at least one senior student was present overseeing our daily experimental operations following all the guidelines. Some of the guidelines are as follows: </p> | + | <div class="column full_size" > |
− | </div>
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− | <div class="column full_size"><!-- 全幅面的文字内容,上面是否留白可选 -->
| + | <h4>Safety training</h4> |
| + | |
| + | <p>All members of our team have taken online courses in biosafety, such as hazardous chemicals, laboratory waste, special equipment safety, accident handling, and passed the laboratory safety examination required by the Assets and Security Department of our university before we entering the lab.</p> |
| + | |
| + | <h4>Lab work</h4> |
| + | |
| + | <p>Our experiments were all performed in the laboratory of Prof. Liang Cai in the School of Life Sciences, which is qualified for Biosafety Level 2. For safety equipment, the lab has biosafety cabinets allowing us to carry out most experiments safely. There is a cabinet to store the hazardous reagents, the key of which only Prof. Cai has, and have a dedicated refrigerator for iGEM to store our experimental materials including plasmids and primers. Fire extinguishers, fire hydrants, emergency spray, and other safety facilities are also available in the lab to deal with different accidental situations.</p> |
| + | |
| + | <p>As the supervisor of the lab, Prof. Cai also provided us with systematic safety guidelines and in-field training before we started the lab affair. Every time we conducted our experiments, at least one senior student was present overseeing our daily experimental operations following all the guidelines. Some of the guidelines were as follows: </p> |
| + | |
| <ol> | | <ol> |
− | <li>The experimental bench and the rest of area should be strictly separated. Everyone performing experiments must wear Lab -gown and nitrile gloves before getting into the experimental area. Masks should also be wearing to prevent E. coli from being taken in. Lab -gown, gloves and any other used personal protection equipment must not be taken out of the experimental area.</li> | + | <li>The experimental bench and the rest of the area should be strictly separated. Everyone performing experiments must wear Lab -gown and nitrile gloves before getting into the experimental area. Masks should also be wearing to prevent <i>E. coli</i> from being taken in. Lab -gown, gloves and any other used personal protective equipment must not be taken out of the experimental area.</li> |
− | <li>Experiments using hazardous chemicals like Ethidium Bromide should be conducted on special bench. While conducting, experimenters must wear an additional pair of nitrile gloves to protect themselves and avoid contaminating the inner gloves.</li> | + | <li> Experiments using hazardous chemicals like Ethidium Bromide should be conducted on a special bench. While conducting, experimenters must wear an additional pair of nitrile gloves to protect themselves and avoid contaminating the inner gloves.</li> |
− | <li>If wanting to use the high-handed sterilization pan, students should ask supervisor or other senior students to operate instead. </li> | + | <li>If wanting to use the high-handed sterilization pan, students should ask the supervisor or other senior students to operate instead. </li> |
− | <li>The last person to leave the lab should make sure that water, electricity, and the air conditioner are shut down, and lock all the doors and windows before leaving. </li> | + | <li>The last person to leave the lab should make sure that water, electricity, and the air conditioner are shut down, and lock all the doors and windows before leaving.</li> |
− | </ol></p> | + | </ol> |
| + | |
| <h4>Avoid contamination</h4> | | <h4>Avoid contamination</h4> |
− | <div class="column full_size" >
| + | <p>All kinds of wastes were disposed of following the laboratory waste disposal policy strictly. Normal laboratory wastes are collected and emptied regularly. Contaminative and toxic waste was separately stored according to their chemical properties waiting for specialists to treat. All containers in contact with bacteria were sterilized before and after use. Experiments that might cause bacteria contamination are performed in Biosafety cabinets. Our culture medium and plates were sealed and preserved in separate refrigerators. Besides, our genetic materials were provided directly from Prof. Cai’s lab or ordered from a local biotechnology company, so there was no transfer or shipment problem involved. We have always transferred our information and experimental data with great deliberation.</p> |
− | <p>All kinds of wastes were disposed following the laboratory waste disposal policy strictly. Normal laboratory wastes are collected and emptied regularly. Contaminative and toxic waste was separately stored according to their chemical properties waiting for specialists to treat. | + | |
− |
| + | |
− | All containers in contact with bacteria were sterilized before and after use. Experiments that might cause bacteria contamination are performed in Biosafety cabinets. Our culture medium and plates were sealed and preserved in separate refrigerators. | + | |
| | | |
− | In addition, our genetic materials are provided directly from Prof. Cai’s lab or ordered from local biotechnology company, so there were no transfer or shipment problem involved. We have always transferred our information and experimental data with great deliberation.</p>
| + | </div> |
− | </div></p> | + | |
| <div class="clear"></div><!-- 一个章节结束需要clear来留白 --> | | <div class="clear"></div><!-- 一个章节结束需要clear来留白 --> |
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| <h2>Project design</h2> | | <h2>Project design</h2> |
− | <div class="column half_size"> | + | <div class="column full_size" > |
− | <img src="https://static.igem.org/mediawiki/2020/8/8b/T--Fudan--map_2.svg" alt="map of calcium intake" />
| + | <p>Most of the organisms and parts we have chosen were widely used and we made throughout safety assessment on them before finally decided to use. And we also introduced several devices to guarantee the safety of both users and the environment.</p> |
− | </div>
| + | |
− | <div class="column half_size">
| + | |
− | <p>Most of organisms and parts we have chosen are widely used and we made through out safety assessment on them before finally decided to use. And we also introduced several devices to guarantee the safety of both users and the environment.</p>
| + | |
| </div> | | </div> |
| <div class="clear"></div><!-- 一个章节结束需要clear来留白 --> | | <div class="clear"></div><!-- 一个章节结束需要clear来留白 --> |
− |
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| + | <div class="column full_size" > |
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| <h4>Nissle 1917</h4> | | <h4>Nissle 1917</h4> |
− | <div class="column full_size" >
| + | <p>We chose <i>E. coli</i> Nissle 1917 as the main chassis to take effect in our project. <i>E. coli</i> Nissle 1917 has a history of 100 years as a licensed medicinal probiotic and is one of the most intensively investigated bacterial strain used in a probiotic preparation today. Its biosafety and effect have since been extensively shown in numerous trials<sup>[1]</sup>. Neither the gene of prominent virulence or the production of toxins have been found in Nissle 1917 and this strain also has proven to lack immunotoxic properties<sup>[2]</sup>. What’s more, Nissle has the ability to express several fitness factors, such as microcins, adhesins and iron uptake systems that facilitate their colonization within the host<sup>[3]</sup>. In addition, Nissle is in Group 1 and has been handled properly in the Safety Level 2 laboratory. To summarize, Nissle 1917 proves to be the perfect chassis for our project. Unfortunately, due to the limited time, we only verified the designed polypeptide chain can be expressed and function normally in <i>E. coli</i>. We hoped to consummate experiments Nissle 1917 in the future.</p> |
− | <p>We chose E. coli Nissle 1917 as the main chassis to take effect in our project. E. coli Nissle 1917 has a history of 100 years as a licensed medicinal probiotic and is one of the most intensively investigated bacterial strain used in a probiotic preparation today. Its biosafety and effect have since been extensively shown in numerous trials.[1] Neither the gene of prominent virulence or the production of toxins have been found in Nissle 1917 and this strain also has proven to lack immunotoxic properties. [2] What’s more, Nissle has the ability to express several fitness factors, such as microcins, adhesins and iron uptake systems that facilitate their colonization within the host.[3] In addition, Nissle is in Group 1 and have been handled properly in Safety Level 2 laboratory. To summarize, Nissle 1917 proves to be perfect chassis for our project. Unfortunately, due to the limited time, we only verify the designed polypeptide chain can be expressed and function normally in E. coli. We hope to consummate experiments Nissle 1917 in the future. | + | |
− | </div>
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− | <div class="clear"></div>
| + | |
− | <h4>E. coli</h4>
| + | |
− | <div class="column full_size" >
| + | |
− | <p>E. coli strain DH5α and BL21 have been used to carry out molecular cloning and obtain the designed plasmids in our project. They are non-pathogenic and can hardly survive in natural environment. Both strains are in Group 1 and have been handled properly in Safety Level 2 laboratory.</p>
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− | </div>
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− | <div class="clear"></div>
| + | |
− | <h4>Kill switch</h4>
| + | |
− | <div class="column full_size" >
| + | |
− | <p>Although our project remains at an experimental stage, no usage outside the lab or clinical trials are applied, we devised some approaches to avoid potential problems. In order to ensure our engineered probiotic will not jeopardize users or the environment, this year, we introduced two kill switches into our project.
| + | |
| | | |
− | To deprive of the survivability of engineered Nissle in the environment when excreted with feces, we introduced the first kill switch. The first kill switch is a cold triggered suicide device, consisting a toxin-antitoxin system as major functional part and an RNA thermometer to regulate it. The toxin RelE has the ability to shut down protein synthesis and cleave mRNA within the ribosomal A site.[4] This toxin can result in cell death and is expressed constantly. The antitoxin RelB is the neutralizer of RelE therefore is able to inhibit the cleavage of mRNA enabling probiotic to survive. [5] RelB is expressed under the control of an RNA thermometer No-Chill 06. Under the body temperature (37℃),No-Chill 06 unfolded and expose its ribosome binding site (RBS)to express antitoxin RelB. While under 30℃, the expression of RelB is suppressed and RelE will kill the probiotic. (Click to see detailed design.)
| + | <h4><i>E. coli</i></h4> |
| | | |
− | The second kill switch is an L-arabinose-activated promoter followed by a translational inhibitor (MazF). It is introduced to enable users to eliminate the engineered bacteria at will, meanwhile it won’t upset native intestinal flora. Whenever users want to stop using our probiotic, they can intake a L-arabinose tablet which has an easy access in health food store.
| + | <p><i>E. coli</i> strain DH5α and BL21 have been used to carry out molecular cloning and obtain the designed plasmids in our project. They are non-pathogenic and can hardly survive in the natural environment. Both strains are in Group 1 and have been handled properly in the Safety Level 2 laboratory.</p> |
− | </p> | + | |
− | </div>
| + | |
− | <div class="clear"></div>
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− | <h4>Future expectation</h4>
| + | |
− | <div class="column full_size" >
| + | |
− | <p>Antibiotics resistance gene present a safety concern and is forbidden in probiotic consumption. However, it has been paid relatively less attention by previous iGEM teams working on probiotics. Antibiotics resistance gene is the most commonly used Selective marker gene. While encoded on plasmid, it has the possibility to transfer to intestinal flora in the host and hurries the development of antibiotic-resistant strains[6]. As Our project remains at an experimental stage that won’t implement experiments in vivo and took the huge expenditures of both cost and time in consider, we still used antibiotics resistance genes to make screenings.
| + | |
− | For commercialization plan in the future, we may try recombinase-mediated DNA insertion (RMDI) to replace antibiotics resistance gene to screen [7] or insert our genetic circuits into genome to inhibit the horizontal transfer directly.
| + | |
− | </p> | + | |
− | </div>
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− | <div class="column full_size"><!-- 全幅面的文字内容,上面是否留白可选 -->
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− | </div>
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− | <div class="clear"></div>
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− |
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− | <h2>COVID-19</h2>
| + | |
− | <div class="column third_size">
| + | |
− | <img src="https://static.igem.org/mediawiki/2020/8/8b/T--Fudan--map_2.svg" alt="map of calcium intake" />
| + | |
− | </div>
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− | <div class="column two_thirds_size">
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− | </div>
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− | <div class="clear"></div><!-- 一个章节结束需要clear来留白 -->
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− | <div class="column full_size">
| + | <h4>Kill Switch</h4> |
− | <h4>Project implementation during pandemic</h4> | + | |
− | <p>Given the outbreak of COVID-19 pandemic, we made extra efforts to protect ourselves and our communities. We strictly complied with all guidance from our university and local government the whole time.
| + | |
| | | |
− | The first thing we ought to consider was to guarantee team members’ own health and safety. On account of no admittance to campus by the university, we converted our meetings to online remote conference. Members that got back to school to carry out wet lab experiments have obtained the permission of the university. Though our university located in very low risk area, we wore masks carefully whenever in public occasions to prevent infection. In the meantime, physical condition of every team members was closely monitored by “Hello Fudan” applet.</p>
| + | <p>Although our project remained at an experimental stage, no usage outside the lab or clinical trials were applied, we devised some approaches to avoid potential problems. In order to ensure our engineered probiotic will not jeopardize the environment or users, this year, we integrated two kinds of Kill Switches into our project.</p> |
− | <h4>HP and education during pandemic</h4>
| + | <p>To deprive of the survivability of engineered Nissle in the environment when excreted from the human intestine, we introduced the first Kill Switch. The first Kill Switch is a cold triggered Kill Switch, consisting of a toxin/antitoxin system as a major functional part and an RNA thermometer to regulate it. (Check to <a href="/Team:Fudan/Design#kill_switch">see the detailed design</a> and our consideration in<a href="/Team:Fudan/Engineering"> engineering</a>.) And bacteria escaped from Kill Switch could be eliminated by the complementary automatic toilet cleaner.</p> |
− | <p>During this special time, we especially emphasize social distancing and focus on online interactions. In integrated human practice, we used online questionnaire to investigate public about the awareness of osteoporosis and calcium absorption. To make better access to the old, our target group, considering their weakness and lack of internet skills, we guide the young to help the old in their family to finish it. Besides, we use e-mails to connect with the osteoporosis filed clinician and relevant professors, changing face-to-face interview to the WeChat voice call. Aim at engaging current osteoporosis issues, we search published surveys which we combine with 2018 China guideline for diagnosis and treatment of senile osteoporosis to help us analyze current situation. For education, we cooperate with Tianjin university Team crating an online life science summer camp for middle school students. To avoid public education events, we design online audio course and textbook to introduce basic biology knowledge.</p>
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| </div> | | </div> |
− | <div class="clear"></div><!-- 一个章节结束需要clear来留白 -->
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− | <h4>Reference</h4> | + | <div class="column half_size"> |
− | <p>[1] Ulrich Sonnenborn, Escherichia coli strain Nissle 1917—from bench to bedside and back: history of a special Escherichia coli strain with probiotic properties, FEMS Microbiology Letters, Volume 363, Issue 19, October 2016, fnw212, https://doi.org/10.1093/femsle/fnw212</p>
| + | <img src="https://static.igem.org/mediawiki/2020/2/25/T--Fudan--img_ks4.svg" alt="cold triggered MazF/MazE Kill Switch under body temperature(37℃)" /> |
− | <p>[2] Behnsen, Judith et al. “Probiotics: properties, examples, and specific applications.” Cold Spring Harbor perspectives in medicine vol. 3,3 a010074. 1 Mar. 2013, doi:10.1101/cshperspect.a010074</p>
| + | </div> |
− | <p>[3] Yu, Xiaoli et al. “Bioengineered Escherichia coli Nissle 1917 for tumour-targeting therapy.” Microbial biotechnology vol. 13,3 (2020): 629-636. doi:10.1111/1751-7915.13523</p>
| + | <div class="column half_size"> |
− | <p>[4] Overgaard M , Borch J , Gerdes K . RelB and RelE of Escherichia coli Form a Tight Complex That Represses Transcription via the Ribbon–Helix–Helix Motif in RelB[J]. Journal of Molecular Biology, 2009, 394(2):0-196.</p>
| + | <img src="https://static.igem.org/mediawiki/2020/a/a4/T--Fudan--img_ks3.svg" alt="cold triggered MazF/MazE Kill Switch outside the body(30℃)" /> |
− | <p>[5] Andreas Bøggild, Sofos N, Andersen K R, et al. The Crystal Structure of the Intact E. coli ReIBE Toxin-Antitoxin Complex Provides the Structural Basis for Conditional Cooperativity[J]. Structure, 2012, 20(10):1641-1648.</p>
| + | </div> |
− | <p>[6] de Melo Pereira, G.V., et al., How to select a probiotic? A review and update of methods and criteria. Biotechnology Advances, 2018. 36(8): p. 2060-2076.</p>
| + | |
− | <p>[7] Turan, S., et al., Recombinase-Mediated Cassette Exchange (RMCE): Traditional Concepts and Current Challenges. Journal of Molecular Biology, 2011. 407(2): p. 193-221.</p>
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− | <div class="highlight decoration_B_full"> | + | |
− | <p>
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− | 章节内容的分段就用p开p关,额外需要关注的可以套一个highlight的框,细看 https://2020.igem.org/Resources/Template_Documentation#highlight
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− | </p>
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− | </div>
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− | <p>
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− | 章节内容的分段就用p开p关
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− | </p>
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− | <p>
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− | 章节内容的分段就用p开p关 <a href="https://URL"> EXAM 外部链接 href尽量使用https </a>
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− | </p>
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− | <ul>
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− | <li>注意这里列表的写法 页面字会变深 突出内容 ul是没有标数字的列表 nts</li>
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− | <li>calcium-supple menting pills don’t haat taste</li>
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− | <li>Vegetables conta列表的写法 页面字会变深 突出内容 ol是编数字的 ul是没有标数字的列表 </li>
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− | </ul>
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− | <p>
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− | 章节内容的分段就用p开p关
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− | </p>
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− | </div><!-- 每个幅面结束都需要 关div --> | + | |
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− | <div class="clear extra_space"></div><!-- 一个章节结束需要clear来留白 如果觉得内容间差异大再加上extra_space --> | + | <div class="column full_size" > |
| + | <h6>Figure 1. Cold triggered MazF/MazE Kill Switch under body temperature (37℃) and outside the body (30℃).</h6></div> |
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− | <h2>又来个章节 sis</h2>
| + | <div class="column full_size" > |
− | <div class="column full_size">
| + | <p>The second Kill Switch is an L-arabinose-activated promoter followed by a translational inhibitor MazF made by 19BNU_China (<a target="_blank" href="http://parts.igem.org/Part:BBa_K3036005">BBa_K3036005</a>). It is introduced to enable users to eliminate the engineered bacteria at will, meanwhile, it won’t upset native intestinal flora. Whenever users want to stop using our probiotic, they can intake an L-arabinose tablet which has easy access from a health food store. </p> |
− | <p>引入文字 ion.</p>
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− | <p>Firstly, the product is designed for oral use and our target user is the elderly. 英文分项不需要ol或ul</p>
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− | <ol>
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− | <li>The produc</li>
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− | <li>The short pep</li>
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− | <li>The expression level of short peptides has a threshold, preventing any excessive calcium absorption that might lead to hypercalcemia 注意句子超长后的回折</li>
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− | <li>There is a kill swit.</li>
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− | </ol>
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− | <p>
| + | <h4>Future expectation</h4> |
− | Based on relevant research, the product is exp 章节内分段 ina. tien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste
| + | |
− | oporosis patienChina has an estimated 70 million osteoporosis pat
| + | |
− | </p>
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− | <p>Compared w 章节内分段 ique advantages tien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste
| + | <p>Antibiotics resistance gene present a safety concern and is forbidden in probiotic consumption. However, it has been paid relatively less attention by previous iGEM teams working on probiotics. Antibiotics resistance gene is the most commonly used Selective marker gene. While encoded on the plasmid, it has the possibility to transfer to intestinal flora in the host and hurries the development of antibiotic-resistant strains<sup>[4]</sup>. Our project remains at an experimental stage that won’t implement experiments in vivo and we took the huge expenditures of both cost and time into consideration, we still used antibiotics resistance genes to make screenings.</p> |
− | oporosis patienChina has an estimated 70 million osteoporosis pat tien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste
| + | <p>For a commercialization plan in the future, we may try recombinase-mediated DNA insertion (RMDI) to replace antibiotics resistance genes to screen<sup>[5]</sup> or insert our genetic circuits into the genome to inhibit the horizontal transfer directly.</p> |
− | oporosis patienChina has an estimated 70 million osteoporosis pat tien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste
| + | </div> |
− | oporosis patienChina has an estimated 70 million osteoporosis pat.
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− | </p>
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− | <table> | + | <div class="clear"></div> |
− | <tr>
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− | <th> Header 1 这是igem hq的表格的写法 </th> <th> Header 2 </th>
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− | </tr>
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− | <td> Content A 1 </td> <td> Content B 1 </td>
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− | <td> Content A 2 </td> <td> Content B 2 这是igem hq的表格的写法。表格都需要说明,之前之后都会有p来文字 </td>
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− | </tr>
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− | </table> | + | |
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− | <p></p><!-- 无文字p是换行隔开,在关闭的html元素间回车是没有用的 --> | + | <h2>Safety during the COVID-19</h2> |
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− | <table class="striped"><!-- 这是另一个版本的表格,间隔行有阴影。一个页面不建议交叉两种格式。太宽表格可以加 responsive-table 方便窄屏 --> | + | <div class="column full_size"> |
− | <tr> | + | <h4>Project implementation during the pandemic</h4> |
− | <th>Name</th> <th>Item Name</th> <th>Item Price</th>
| + | |
− | </tr>
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− | <tr>
| + | |
− | <td>Alvin</td> <td>Eclair</td> <td>$0.87</td>
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− | </tr>
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− | <tr>
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− | <td>Alan</td> <td>Jellybean</td> <td>$3.76</td>
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− | </tr>
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− | <tr>
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− | <td>Jonathan</td> <td>Lollipop</td> <td>$7.00</td>
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− | </tr>
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− | </table> | + | |
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− | <p>Calciu 章节内分段 fety. tien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste | + | <p>Given the outbreak of the COVID-19 pandemic, we made extra efforts to protect ourselves and our communities. We strictly complied with all guidance from our university and local government the whole time. The first thing we ought to consider was to guarantee team members’ own health and safety. On account of no admittance to campus by the university, we converted our meetings to online remote conferences. Members that got back to school to carry out wet lab experiments have obtained the permission of the university. Though our university is located in a very low-risk area, we wore masks carefully whenever in public occasions to prevent infection. In the meantime, the physical condition of every team member was closely monitored by the “Hello Fudan” applet.</p> |
− | oporosis patienChina has an estima
| + | |
− | ted 70 million osteoporosis pat tien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste
| + | |
− | oporosis patienChina has an estimated 70 mill
| + | |
− | ion osteoporosis pattien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste
| + | |
− | oporosis patienChina has an estimated 70 million osteoporosis pat
| + | |
− | </p>
| + | |
| | | |
− | <p>From the us 章节内分段 etition.</p>
| + | <h4>HP and education during pandemic</h4> |
− | </div>
| + | <p>During this particular time, we especially emphasize social distancing and focus on online interactions. In integrated human practice, we used an online questionnaire to investigate the public about osteoporosis awareness and calcium absorption. To better access the old, our target group, considering their weakness and lack of internet skills, we guide the young to help the aged in their family finish it. Besides, we use e-mails to connect with the osteoporosis field clinician and relevant professors, changing face-to-face interviews with the WeChat voice call. Aiming to engage current osteoporosis issues, we investigated published surveys, combining them with the 2018 China guideline for diagnosis and treatment of senile osteoporosis to analyze the current situation. We cooperate with Tianjin university Team for education, creating an online life science summer camp for middle school students. To avoid public education events, we design an online audio course and textbook to introduce basic biology knowledge.</p> |
| + | </div> |
| | | |
− | <div class="clear"></div><!-- 一个章节结束需要clear来留白 -->
| + | <div class="clear"></div><!-- 一个章节结束需要clear来留白 --> |
| | | |
− | <h2>再来例子 Market Analysis</h2> | + | <div class="column full_size"><div class="highlight decoration_background decoration_B_full"> |
− | <div class="column half_size">
| + | <p>We have paid high attention to safety issues on project design, project implementation and health precaution during the COVID-19 pandemic following the instruction of the Safety and Security Community. Our project was accomplished successfully without bringing potential risks to ourselves, our communities and the environment.</p> |
− | <p>文本在左侧一半,宽屏 According to the Chinese Dietetic Sociietary Nutrient Intakes of Chinese Residents", the calcium requirement for infants and young children increases with age, from 300mg/day to 800mg/day, and the calcium requirement for adolescents reaches 1000mg/day. The amof the required intake, with the small exception of some coastal and pastoral areas 图片在右侧一半</p> | + | </div></div> |
| | | |
− | <table> | + | <div class="clear"></div><!-- 一个章节结束需要clear来留白 --> |
− | <tr>
| + | <h2>Reference</h2> |
− | <th> 这是igem hq的表格的写法 </th> <th> Header 2 </th> <th> Header 3 </th>
| + | <div class="column full_size"> |
− | </tr> | + | <p>[1] Ulrich Sonnenborn, <i>Escherichia coli</i> strain Nissle 1917—from bench to bedside and back: history of a special <i>Escherichia coli</i> strain with probiotic properties, FEMS Microbiology Letters, Volume 363, Issue 19, October 2016, fnw212, https://doi.org/10.1093/femsle/fnw212 <br/> |
− | <tr> | + | [2] Behnsen, Judith et al. “Probiotics: properties, examples, and specific applications.” Cold Spring Harbor perspectives in medicine vol. 3,3 a010074. 1 Mar. 2013, doi:10.1101/cshperspect.a010074<br/> |
− | <td> Content A 1 </td> <td> Content B 1 </td> <td> Content C 1 </td>
| + | [3] Yu, Xiaoli et al. “Bioengineered <i>Escherichia coli</i> Nissle 1917 for tumour-targeting therapy.” Microbial biotechnology vol. 13,3 (2020): 629-636. doi:10.1111/1751-7915.13523<br/> |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td colspan=2> 列融合 </td> <td> Content C 2 这是igem hq的表格的写法。半幅或者2/3幅可以用。<br/>行融合<br/>就<br/>用br了 </td>
| + | |
− | </tr>
| + | |
− | </table> | + | |
| | | |
− | </div>
| + | [4] de Melo Pereira, G.V., et al., How to select a probiotic? A review and update of methods and criteria. Biotechnology Advances, 2018. 36(8): p. 2060-2076.<br/> |
− | <div class="column half_size">
| + | [5] Turan, S., et al., Recombinase-Mediated Cassette Exchange (RMCE): Traditional Concepts and Current Challenges. Journal of Molecular Biology, 2011. 407(2): p. 193-221.</p> |
− | <img src="https://static.igem.org/mediawiki/2020/0/00/T--Fudan--calcium_intake_graph.svg" alt="calcium intake graph">
| + | </div> |
− | </div>
| + | <div class="clear"></div><!-- 一个章节结束需要clear来留白 --> |
| | | |
− | <div class="clear extra_space"></div><!-- 一个章节结束需要clear来留白 因为马上最后一幅,建议extra_space -->
| + | <div class="column full_size"><div id="FudanSignature"> |
− | | + | <p>Signature: <a href="/Team:Fudan/Attributions#ZhangJQ" class="z-depth-2">Jingqi</a> |
− | <div class="column full_size">
| + | </p> |
− | <p>每页结束需要小结,可以 ul ol 列表highlight,可以是每页第一段的另一种写法fety. tien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste
| + | </div></div> |
− | oporosis patienChina has an estima
| + | |
− | ted 70 million osteoporosis pat tien China has an estimated 70 million osteoporosis patien China has an estimated 70 million osteoporosis patienChina has an estimated 70 million oste
| + | |
− | oporosis patienChina has an estimated 70 mi 每页结束需要小结,可以 ul ol 列表highlight,可以是每页第一段的另一种写法</p>
| + | |
− | </div>
| + | |
− | | + | |
− | <div class="column full_size"><div id="FudanSignature">
| + | |
− | <p>Signature: <a href="/Team:Fudan/Attributions#ZhangJQ" class="z-depth-2">Jingqi</a>
| + | |
− | </p>
| + | |
− | </div></div>
| + | |
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| <a href="http://www.yfc.cn/" target="_blank"><img class="col s3 m6 l4 FudanSponsors" alt="Yunfeng Capital" src="https://static.igem.org/mediawiki/2020/b/ba/T--Fudan--YFcapital.svg"> </a> | | <a href="http://www.yfc.cn/" target="_blank"><img class="col s3 m6 l4 FudanSponsors" alt="Yunfeng Capital" src="https://static.igem.org/mediawiki/2020/b/ba/T--Fudan--YFcapital.svg"> </a> |
| <a href="http://www.fudan.edu.cn/en/" target="_blank"><img alt="Fudan University" class="col s3 m6 l4 FudanSponsors" src="https://static.igem.org/mediawiki/2020/9/9d/T--Fudan--sponsor2.svg"> </a> | | <a href="http://www.fudan.edu.cn/en/" target="_blank"><img alt="Fudan University" class="col s3 m6 l4 FudanSponsors" src="https://static.igem.org/mediawiki/2020/9/9d/T--Fudan--sponsor2.svg"> </a> |
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| <ul><li><a href="/Team:Fudan/Description">Description</a></li> | | <ul><li><a href="/Team:Fudan/Description">Description</a></li> |
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| <ul><li><a href="/Team:Fudan/Safety">Safety</a></li> | | <ul><li><a href="/Team:Fudan/Safety">Safety</a></li> |
| <li><a href="/Team:Fudan/Poster">Poster</a></li> | | <li><a href="/Team:Fudan/Poster">Poster</a></li> |
− | <li><a href="#">Video</a></li> | + | <li><a href="https://video.igem.org/videos/watch/7a0fee51-a637-4caf-aa97-a1d9bcafd3ba" target="_blank">Promotion</a></li> |
| + | <li><a href="#" target="_blank">Presentation</a></li> |
| + | <li><a href="/Team:Fudan/Entrepreneurship">Entrepreneurship</a></li> |
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| <li><a href="/Team:Fudan/Engineering">Engineering</a></li> | | <li><a href="/Team:Fudan/Engineering">Engineering</a></li> |
| <li><a href="/Team:Fudan/Measurement">Measurement</a></li> | | <li><a href="/Team:Fudan/Measurement">Measurement</a></li> |
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| <li><a href="/Team:Fudan/Attributions">Attributions</a></li> | | <li><a href="/Team:Fudan/Attributions">Attributions</a></li> |
| <li><a href="/Team:Fudan/Partnership">Partnership</a></li> | | <li><a href="/Team:Fudan/Partnership">Partnership</a></li> |
| <li><a href="/Team:Fudan/Acknowledgement">Acknowledgement</a></li> | | <li><a href="/Team:Fudan/Acknowledgement">Acknowledgement</a></li> |
− | <li><a href="https://2019.igem.org/Team:Fudan-TSI/Heritage" target="_blank">Heritage</a></li> | + | <li><a href="/Team:Fudan/Heritage">Heritage</a></li> |
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