Difference between revisions of "Team:XMU-China/Parts"

 
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     <link href="https://cdn.bootcss.com/font-awesome/4.7.0/css/font-awesome.min.css" rel="stylesheet">
 
     <link href="https://cdn.bootcss.com/font-awesome/4.7.0/css/font-awesome.min.css" rel="stylesheet">
 
     <script src="https://2020.igem.org/Team:XMU-China/js/jquery?action=raw&amp;ctype=text/javascript"></script>
 
     <script src="https://2020.igem.org/Team:XMU-China/js/jquery?action=raw&amp;ctype=text/javascript"></script>
−
     <script src="https://2019.igem.org/Team:XMU-China/js/bootstrap?action=raw&amp;ctype=text/javascript"></script>
+
     <script src="https://2020.igem.org/Team:XMU-China/js/bootstrap?action=raw&amp;ctype=text/javascript"></script>
 
</head>
 
</head>
 
<body>
 
<body>
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     <div class="Quick-navigation_word">
 
     <div class="Quick-navigation_word">
 
         <img  src="https://static.igem.org/mediawiki/2020/c/c3/T--XMU-China--right30.png">
 
         <img  src="https://static.igem.org/mediawiki/2020/c/c3/T--XMU-China--right30.png">
−
         <a href="#Basic_Parts" class="Quick-navigation-item">
+
         <a href="#BP" class="Quick-navigation-item">
 
             <img id="turn_img" src="https://static.igem.org/mediawiki/2020/d/d9/T--XMU-China--right31.png">
 
             <img id="turn_img" src="https://static.igem.org/mediawiki/2020/d/d9/T--XMU-China--right31.png">
−
             <a href="#Basic_Parts"id="Quick_A">Basic Parts</a></a>
+
             <a href="#BP"id="Quick_A">Basic Parts</a></a>
−
         <a href="#Composite_Parts" class="Quick-navigation-item" >
+
         <a href="#CP" class="Quick-navigation-item" >
 
             <img id="turn_img" src="https://static.igem.org/mediawiki/2020/f/f6/T--XMU-China--right32.png">
 
             <img id="turn_img" src="https://static.igem.org/mediawiki/2020/f/f6/T--XMU-China--right32.png">
−
             <a href="#Composite_Parts" id="Quick_B">Composite Parts</a></a>
+
             <a href="#CP" id="Quick_B">Composite Parts</a></a>
−
         <a href="#Part_Collection" class="Quick-navigation-item">
+
         <a href="#PC" class="Quick-navigation-item">
 
             <img id="turn_img" src="https://static.igem.org/mediawiki/2020/f/fa/T--XMU-China--right33.png">
 
             <img id="turn_img" src="https://static.igem.org/mediawiki/2020/f/fa/T--XMU-China--right33.png">
−
             <a href="#Part_Collection" id="Quick_C">Part Collection</a></a>
+
             <a href="#PC" id="Quick_C">Part Collection</a></a>
 
     </div>
 
     </div>
 
</nav>
 
</nav>
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         <div class="wrap">
 
         <div class="wrap">
 
             <div class="team">
 
             <div class="team">
−
                 <a href="https://2019.igem.org/Team:XMU-China">
+
                 <a href="https://2020.igem.org/Team:XMU-China">
 
                     <img src="https://static.igem.org/mediawiki/2020/c/cb/T--XMU-China--logo.png" alt="">
 
                     <img src="https://static.igem.org/mediawiki/2020/c/cb/T--XMU-China--logo.png" alt="">
 
                 </a>
 
                 </a>
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                             <a href="https://2020.igem.org/Team:XMU-China/Parts"><li>Parts</li></a>
 
                             <a href="https://2020.igem.org/Team:XMU-China/Parts"><li>Parts</li></a>
 
                             <a href="https://2020.igem.org/Team:XMU-China/Contribution"><li>Contributions</li></a>
 
                             <a href="https://2020.igem.org/Team:XMU-China/Contribution"><li>Contributions</li></a>
−
                             <a href="https://2020.igem.org/Team:XMU-China/Engineering"><li>Engeering Success</li></a>
+
                             <a href="https://2020.igem.org/Team:XMU-China/Engineering"><li>Engineering Success</li></a>
 
                             <a href="https://2020.igem.org/Team:XMU-China/Improve"><li>Improve</li></a>
 
                             <a href="https://2020.igem.org/Team:XMU-China/Improve"><li>Improve</li></a>
 
                         </ul>
 
                         </ul>
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                         <a href="" class="a1">Awards</a>
 
                         <a href="" class="a1">Awards</a>
 
                         <ul class="two">
 
                         <ul class="two">
−
                             <a href="https://2019.igem.org/Team:XMU-China/Judging"><li>Judging</li></a>
+
<a href="https://2020.igem.org/Team:XMU-China/Awards"><li>Awards</li></a>
 +
                             <a href="https://2020.igem.org/Team:XMU-China/Judging"><li>Judging</li></a>
 
                             <a href="https://2020.igem.org/Team:XMU-China/Human_Practices"><li>Best Integrated Human Practices</li></a>
 
                             <a href="https://2020.igem.org/Team:XMU-China/Human_Practices"><li>Best Integrated Human Practices</li></a>
 
                             <a href="https://2020.igem.org/Team:XMU-China/Education"><li>Best Education</li></a>
 
                             <a href="https://2020.igem.org/Team:XMU-China/Education"><li>Best Education</li></a>
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                             <a href="https://2020.igem.org/Team:XMU-China/Model"><li>Best Model</li></a>
 
                             <a href="https://2020.igem.org/Team:XMU-China/Model"><li>Best Model</li></a>
 
                             <a href="https://2020.igem.org/Team:XMU-China/Hardware"><li>Best Hardware</li></a>
 
                             <a href="https://2020.igem.org/Team:XMU-China/Hardware"><li>Best Hardware</li></a>
−
                             <a href="https://2020.igem.org/Team:XMU-China/Entrepreneurship#Basic"><li>Best Basic Parts</li></a>
+
                             <a href="https://2020.igem.org/Team:XMU-China/Parts#Basic"><li>Best Basic Parts</li></a>
−
                             <a href="https://2020.igem.org/Team:XMU-China/Entrepreneurship#Composite"><li>Best Composite Parts</li></a>
+
                             <a href="https://2020.igem.org/Team:XMU-China/Parts#Composite"><li>Best Composite Parts</li></a>
−
                             <a href="https://2020.igem.org/Team:XMU-China/Entrepreneurship#Collection"><li>Best Part Collection</li></a>
+
                             <a href="https://2020.igem.org/Team:XMU-China/Parts#Collection"><li>Best Part Collection</li></a>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
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     <section class="js-scroll-step">
 
     <section class="js-scroll-step">
 
         <div class="headline"><br></div>
 
         <div class="headline"><br></div>
−
         <p class="indent">This year, among the 41 parts designed, ten are basic parts and the other are composite ones. All of the parts are designed by ourselves, and most of them have been tested to insure their reliability. You can <span><a  class="click_here" href="https://2019.igem.org/Team:XMU-China/Design"> click here </a></span> to learn more about our project to see their specific function, or click on the part numbers when browse website (<span><a class="click_here" href="http://parts.igem.org/Main_Page"> parts.igem.org </a></span>).</p>
+
         <p class="indent">This year, among the 103 parts designed, 44 of them are basic parts and the rest are composite parts. All of the parts are designed by ourselves, and most of them have been tested to insure their reliability. You can <span><a  class="click_here" href="https://2020.igem.org/Team:XMU-China/Design"> click here </a></span> to learn more about our project to see their specific function, or click on the part numbers when browse website (<span><a class="click_here" href="http://parts.igem.org/Main_Page"> parts.igem.org </a></span>).</p>
 
     </section>
 
     </section>
−
     <section id="Basic_Parts" class="js-scroll-step">
+
     <section id="BP" class="js-scroll-step">
 
         <div class="headline">
 
         <div class="headline">
−
             <a class="share" href="https://2019.igem.org/Team:XMU-China/Basic_Part"> Basic Parts</a>
+
             Basic Parts
 
         </div>
 
         </div>
−
         <p class="indent">Among the ten basic parts designed this year by ourselves, <span><a class="click_here" href="http://parts.igem.org/Part:BBa_K2922040 ">BBa_K2922040 </a></span> is our favorite one, which comes from human genomes. The gene enables engineering bacteria to form a vesicle which will have great applications in the enrichment of substrate (such as metal ions) through extracellular membrane and the enhancement of cell stress resistance. Besides, many other basic parts have also been designed to construct our project.
+
         <p class="indent">Among the 44 basic parts designed this year by ourselves, <a class="click_here" href="http://parts.igem.org/Part:BBa_K3332011">BBa_K3332011</a> is our favorite one, which comes from homo liver. The gene enables engineering bacteria to efficiently catalyze the reaction of reducing glyoxalic acid and consuming NADPH. Besides, many other basic parts have also been designed to construct our project.
 
         </p>
 
         </p>
 
         <br>
 
         <br>
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             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332011">BBa_K3332011</a></td>
 
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332011">BBa_K3332011</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
−
             <td>GRHPR-his-tag</td>
+
             <td>grhpr-his-tag</td>
 
             <td>Xiaoyu Wang</td>
 
             <td>Xiaoyu Wang</td>
 
             <td>1005</td>
 
             <td>1005</td>
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             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332000">BBa_K3332000</a></td>
 
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332000">BBa_K3332000</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
−
             <td>iNAP-his-tag</td>
+
             <td>inap-his-tag</td>
 
             <td>Shuling Xiao</td>
 
             <td>Shuling Xiao</td>
 
             <td>1167</td>
 
             <td>1167</td>
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         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922001">BBa_K3332001</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332001">BBa_K3332001</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
−
             <td>His-tag-iNAP</td>
+
             <td>his-tag-inap</td>
 
             <td>Shuling Xiao</td>
 
             <td>Shuling Xiao</td>
 
             <td>1167</td>
 
             <td>1167</td>
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         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922002">BBa_K3332002</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332002">BBa_K3332002</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
−
             <td>INPNC-iNAP</td>
+
             <td>inpnc-inap</td>
 
             <td>Shuling Xiao</td>
 
             <td>Shuling Xiao</td>
 
             <td>2128</td>
 
             <td>2128</td>
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         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922024">BBa_K3332003</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332003">BBa_K3332003</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
−
             <td>Lpp-OmpA-iNAP</td>
+
             <td>lpp-ompA-inap</td>
 
             <td>Shuling Xiao</td>
 
             <td>Shuling Xiao</td>
 
             <td>1614</td>
 
             <td>1614</td>
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         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922025">BBa_K3332004</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332004">BBa_K3332004</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
−
             <td>iNAP-AIDA</td>
+
             <td>inap-aida</td>
 
             <td>Shuling Xiao</td>
 
             <td>Shuling Xiao</td>
 
             <td>2631</td>
 
             <td>2631</td>
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         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922026">BBa_K3332005</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332005">BBa_K3332005</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
−
             <td>iNAP-BrkA</td>
+
             <td>inap-brkA</td>
 
             <td>Shuling Xiao</td>
 
             <td>Shuling Xiao</td>
 
             <td>2547</td>
 
             <td>2547</td>
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         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922027">BBa_K3332006</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332006">BBa_K3332006</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
−
             <td>GOX-his-tag</td>
+
             <td>gox-his-tag</td>
 
             <td>Xiaoyu Wang</td>
 
             <td>Xiaoyu Wang</td>
 
             <td>951</td>
 
             <td>951</td>
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         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922028">BBa_K3332007</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332007">BBa_K3332007</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
−
             <td>INPNC-GOX</td>
+
             <td>inpnc-gox</td>
 
             <td>Xiaoyu Wang</td>
 
             <td>Xiaoyu Wang</td>
 
             <td>1908</td>
 
             <td>1908</td>
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         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332008</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332008">BBa_K3332008</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
−
             <td>Lpp-OmpA-GOX</td>
+
             <td>lpp-ompA-gox</td>
 
             <td>Xiaoyu Wang</td>
 
             <td>Xiaoyu Wang</td>
 
             <td>1398</td>
 
             <td>1398</td>
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         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332009</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332009">BBa_K3332009</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
−
             <td>GOX-AIDA</td>
+
             <td>gox-aida</td>
 
             <td>Xiaoyu Wang</td>
 
             <td>Xiaoyu Wang</td>
 
             <td>2415</td>
 
             <td>2415</td>
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         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332010</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332010">BBa_K3332010</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
−
             <td>GOX-BrkA</td>
+
             <td>gox-brkA</td>
 
             <td>Xiaoyu Wang</td>
 
             <td>Xiaoyu Wang</td>
 
             <td>2331</td>
 
             <td>2331</td>
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         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332012</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332012">BBa_K3332012</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
−
             <td>INPNC-GRHPR</td>
+
             <td>inpnc-grhpr</td>
 
             <td>Shi Zhang</td>
 
             <td>Shi Zhang</td>
 
             <td>1962</td>
 
             <td>1962</td>
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         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332013</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332013">BBa_K3332013</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
−
             <td>Lpp-OmpA-GRHPR</td>
+
             <td>lpp-ompA-grhpr</td>
 
             <td>Shi Zhang</td>
 
             <td>Shi Zhang</td>
 
             <td>1452</td>
 
             <td>1452</td>
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         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332014</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332014">BBa_K3332014</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
−
             <td>GRHPR-AIDA</td>
+
             <td>grhpr-aida</td>
 
             <td>Shi Zhang</td>
 
             <td>Shi Zhang</td>
 
             <td>2469</td>
 
             <td>2469</td>
Line 265: Line 266:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332015</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332015">BBa_K3332015</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
−
             <td>GRHPR-BrkA</td>
+
             <td>grhpr-brkA</td>
 
             <td>Shi Zhang</td>
 
             <td>Shi Zhang</td>
 
             <td>2385</td>
 
             <td>2385</td>
Line 273: Line 274:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332016</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332016">BBa_K3332016</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
−
             <td>INPNC-GFP</td>
+
             <td>inpnc-egfp</td>
 
             <td>Shi Zhang</td>
 
             <td>Shi Zhang</td>
 
             <td>1695</td>
 
             <td>1695</td>
Line 281: Line 282:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332017</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332017">BBa_K3332017</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
−
             <td>Lpp-OmpA-GFP</td>
+
             <td>lpp-ompA-egfp</td>
 
             <td>Shi Zhang</td>
 
             <td>Shi Zhang</td>
 
             <td>1185</td>
 
             <td>1185</td>
Line 289: Line 290:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332018</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332018">BBa_K3332018</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
−
             <td>GFP-AIDA</td>
+
             <td>egfp-aida</td>
 
             <td>Yixian Zheng</td>
 
             <td>Yixian Zheng</td>
 
             <td>2199</td>
 
             <td>2199</td>
Line 297: Line 298:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332019</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332019">BBa_K3332019</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
−
             <td>GFP-BrkA</td>
+
             <td>egfp-brkA</td>
 
             <td>Yixian Zheng</td>
 
             <td>Yixian Zheng</td>
 
             <td>2252</td>
 
             <td>2252</td>
Line 305: Line 306:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332020</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332020">BBa_K3332020</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
 
             <td>phnE<sub>1</sub></td>
 
             <td>phnE<sub>1</sub></td>
Line 313: Line 314:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332021</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332021">BBa_K3332021</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
 
             <td>phnE<sub>2</sub></td>
 
             <td>phnE<sub>2</sub></td>
Line 321: Line 322:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332022</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332022">BBa_K3332022</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
 
             <td>phnC</td>
 
             <td>phnC</td>
Line 329: Line 330:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332023</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332023">BBa_K3332023</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
 
             <td>phnD</td>
 
             <td>phnD</td>
Line 337: Line 338:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332024</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332024">BBa_K3332024</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
 
             <td>phnJ</td>
 
             <td>phnJ</td>
Line 345: Line 346:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332025</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332025">BBa_K3332025</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
 
             <td>phnJ_mut21</td>
 
             <td>phnJ_mut21</td>
Line 353: Line 354:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332026</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332026">BBa_K3332026</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
 
             <td>phnJ_mut40&16</td>
 
             <td>phnJ_mut40&16</td>
Line 361: Line 362:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332027</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332027">BBa_K3332027</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
 
             <td>phnJ_mut45</td>
 
             <td>phnJ_mut45</td>
Line 369: Line 370:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332028</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332028">BBa_K3332028</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
 
             <td>phnO-his-tag</td>
 
             <td>phnO-his-tag</td>
Line 377: Line 378:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332029</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332029">BBa_K3332029</a></td>
 
             <td>DNA</td>
 
             <td>DNA</td>
−
             <td>OmpA 5'UTR-phnF 0.97-Hfq binding sequence</td>
+
             <td>ompA 5'UTR-phnF 0.97-hfp binding sequence</td>
 
             <td>Zhichun Yang</td>
 
             <td>Zhichun Yang</td>
 
             <td>223</td>
 
             <td>223</td>
Line 385: Line 386:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332030</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332030">BBa_K3332030</a></td>
 
             <td>DNA</td>
 
             <td>DNA</td>
−
             <td>OmpA 5'UTR-phnF 0.85-Hfq binding sequence</td>
+
             <td>ompA 5'UTR-phnF 0.85-hfp binding sequence</td>
 
             <td>Ruomeng Bi</td>
 
             <td>Ruomeng Bi</td>
 
             <td>223</td>
 
             <td>223</td>
Line 393: Line 394:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332031</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332031">BBa_K3332031</a></td>
 
             <td>DNA</td>
 
             <td>DNA</td>
−
             <td>OmpA 5'UTR-phnF 0.75-Hfq binding sequence</td>
+
             <td>ompA 5'UTR-phnF 0.75-hfp binding sequence</td>
 
             <td>Ruomeng Bi</td>
 
             <td>Ruomeng Bi</td>
 
             <td>223</td>
 
             <td>223</td>
Line 401: Line 402:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332032</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332032">BBa_K3332032</a></td>
 
             <td>DNA</td>
 
             <td>DNA</td>
−
             <td>OmpA 5'UTR-phnJ 0.94-Hfq binding sequence</td>
+
             <td>ompA 5'UTR-phnJ 0.94-hfp binding sequence</td>
 
             <td>Ruomeng Bi</td>
 
             <td>Ruomeng Bi</td>
 
             <td>223</td>
 
             <td>223</td>
Line 409: Line 410:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332033</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332033">BBa_K3332033</a></td>
 
             <td>DNA</td>
 
             <td>DNA</td>
−
             <td>OmpA 5'UTR-phnJ 0.69-Hfq binding sequence</td>
+
             <td>ompA 5'UTR-phnJ 0.69-hfp binding sequence</td>
 
             <td>Ruomeng Bi</td>
 
             <td>Ruomeng Bi</td>
 
             <td>223</td>
 
             <td>223</td>
Line 417: Line 418:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332034</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332034">BBa_K3332034</a></td>
 
             <td>DNA</td>
 
             <td>DNA</td>
−
             <td>PLtetO promoter</td>
+
             <td>pLtetO-1 promoter</td>
 
             <td>Ruomeng Bi</td>
 
             <td>Ruomeng Bi</td>
 
             <td>142</td>
 
             <td>142</td>
Line 425: Line 426:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332035</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332035">BBa_K3332035</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
 
             <td>E0020-ssrAtag（mf-lon）</td>
 
             <td>E0020-ssrAtag（mf-lon）</td>
Line 433: Line 434:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332036</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332036">BBa_K3332036</a></td>
 
             <td>RBS</td>
 
             <td>RBS</td>
 
             <td>RBS1</td>
 
             <td>RBS1</td>
Line 441: Line 442:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332037</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332037">BBa_K3332037</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
 
             <td>LacI-ssrAtag(mf-lon)</td>
 
             <td>LacI-ssrAtag(mf-lon)</td>
Line 449: Line 450:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332038</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332038">BBa_K3332038</a></td>
 
             <td>DNA</td>
 
             <td>DNA</td>
−
             <td>Ptrc-2 promoter</td>
+
             <td>pTrc-2 promoter</td>
 
             <td>Jinzhu Mao</td>
 
             <td>Jinzhu Mao</td>
 
             <td>91</td>
 
             <td>91</td>
Line 457: Line 458:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332039</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332039">BBa_K3332039</a></td>
 
             <td>Coding</td>
 
             <td>Coding</td>
 
             <td>tetR</td>
 
             <td>tetR</td>
Line 465: Line 466:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332040</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332040">BBa_K3332040</a></td>
 
             <td>DNA</td>
 
             <td>DNA</td>
−
             <td>Ptrc-2 derivative promoter</td>
+
             <td>pTrc-2 derivative promoter</td>
 
             <td>Ruomeng Bi</td>
 
             <td>Ruomeng Bi</td>
 
             <td>130</td>
 
             <td>130</td>
Line 473: Line 474:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332042</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332042">BBa_K3332042</a></td>
 
             <td>DNA</td>
 
             <td>DNA</td>
 
             <td>Formaldehyde_derivative-1 promoter</td>
 
             <td>Formaldehyde_derivative-1 promoter</td>
Line 481: Line 482:
 
         <tr>
 
         <tr>
 
         <td></td>
 
         <td></td>
−
         <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332043</a></td>
+
         <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332043">BBa_K3332043</a></td>
 
         <td>DNA</td>
 
         <td>DNA</td>
 
         <td>Formaldehyde_derivative-2 promoter</td>
 
         <td>Formaldehyde_derivative-2 promoter</td>
Line 489: Line 490:
 
         <tr>
 
         <tr>
 
             <td></td>
 
             <td></td>
−
             <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K3332044</a></td>
+
             <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332044">BBa_K3332044</a></td>
 
             <td>DNA</td>
 
             <td>DNA</td>
 
             <td>Formaldehyde_derivative-3 promoter</td>
 
             <td>Formaldehyde_derivative-3 promoter</td>
Line 498: Line 499:
 
</div>
 
</div>
 
     </section>
 
     </section>
−
     <section id="Part_Collection" class="js-scroll-step">
+
     <section id="CP" class="js-scroll-step">
 
         <div class="headline">
 
         <div class="headline">
−
             <a class="share" href="https://2019.igem.org/Team:XMU-China/Part_Collection"> Part Collection</a>
+
             Composite Parts
 
         </div>
 
         </div>
−
         <p class="indent">This year, we have formulated a Part Collection related to secretion of intracellular proteins, which includes two major parts: one is secretion achieved with the help of YebF in the form of fusion proteins, the other is the expression of Kil protein by promoters of different intensities, thus changing cell permeability to different degrees and finally realizing secretion. For our Part Collection, the efficiency of three different intracellular proteins we utilized were well tested, recorded in detail and completely uploaded to the registry, which would be instructive in the field of design and characterization for many teams.
+
         <p class="indent" style="margin-bottom:0;">A series of composite parts have been designed this year, and most of them are useful for our project. And our favorite composite part is <span><a class="click_here" href="http://parts.igem.org/Part:BBa_K3332057 ">BBa_K3332057 </a></span>, which contains the sequence of the anchor protein—INPNC to anchor our favorite basic part—GRHPR. </p>
−
        </p>
+
<p class="indent" style="margin-bottom:0;">The anchor protein is from a kind of autotransporter family which can be anchored to the membrane and work as a bacterial display system. The cleaved passenger domain remains adhered on the bacterial surface and it is conducive to keep the displayed exogenous proteins associated onto the cell. Thus, the functionalized bacteria can be recycled/re-used conveniently.</p><p class="indent">Throughout the season we’ve combined several surface display systems with GRHPR and verified the function of detecting NADPH outside the cell. After a series of experiments, INPNC is determined as the most suitable anchor protein for GRHPR.</p>
 
         <br>
 
         <br>
 
         <div class="table_container">
 
         <div class="table_container">
−
            <table class="table">
+
    <table class="table">
−
                <tr><span>
+
        <tr>
−
                        <th>Register Name</th>
+
            <th>Favorite</th>
−
                        <th>Code used in Lab</th>
+
            <th>Name</th>
−
                        <th>Description</th>
+
            <th>Type</th>
−
                        <th>Types</th>
+
            <th>Description</th>
−
                        <th>Lenth</th>
+
            <th>Designer</th>
−
                </tr>
+
            <th>Length</th>
−
                <tr>
+
        </tr>
−
                    <td><a href="http://parts.igem.org/Part:BBa_K2922000">BBa_K2922000</a></td>
+
        <tr>
−
                    <td>y-bgl1A</td>
+
            <td>R</td>
−
                    <td>The protein beta-glucosidase<br> with protein YebF fused to its<br> N-terminus by GS Linker.</td>
+
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332057">BBa_K3332057</a></td>
−
                    <td>Coding</td>
+
            <td>Composite</td>
−
                    <td>1764</td>
+
            <td>J23100-B0034-inpnc-grhpr</td>
−
                </tr>
+
            <td>Qingliu Wang</td>
−
                <tr>
+
            <td>2160</td>
−
                    <td><a href="http://parts.igem.org/Part:BBa_K2922001">BBa_K2922001</a></td>
+
        </tr>
−
                    <td>y-cenA</td>
+
        <tr>
−
                    <td>The protein endoglucanase A<br>
+
            <td></td>
−
                        with protein YebF fused to its <br>
+
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332045">BBa_K3332045</a></td>
−
                        N-terminus by GS Linker.</td>
+
            <td>Composite</td>
−
                    <td>Coding</td>
+
            <td>J23100-B0034-inap-his-tag-B0015</td>
−
                    <td>1731</td>
+
            <td>Shengyang Zhang</td>
−
                </tr>
+
            <td>1365</td>
−
                <tr>
+
        </tr>
−
                    <td><a href="http://parts.igem.org/Part:BBa_K2922002">BBa_K2922002</a></td>
+
        <tr>
−
                    <td>y-cex</td>
+
            <td></td>
−
                    <td>The protein exoglucanase<br>
+
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332046">BBa_K3332046</a></td>
−
                        with protein YebF fused to its<br>
+
            <td>Composite</td>
−
                        N-terminus by GS Linker. </td>
+
            <td>J23100-B0034-his-tag-inap-B0015</td>
−
                    <td>Coding</td>
+
            <td>Shengyang Zhang</td>
−
                    <td>1839</td>
+
            <td>1365</td>
−
                </tr>
+
        </tr>
−
                <tr>
+
        <tr>
−
                    <td><a href="http://parts.igem.org/Part:BBa_K2922009">BBa_K2922009</a></td>
+
            <td></td>
−
                    <td>109-kil</td>
+
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332047">BBa_K3332047</a></td>
−
                    <td>The expression of Kil protein<br>
+
            <td>Composite</td>
−
                        by J23109  promoter</td>
+
            <td>J23100-B0034-inpnc-inap</td>
−
                    <td>Composite</td>
+
            <td>Shengyang Zhang</td>
−
                    <td>248</td>
+
            <td>2328</td>
−
                </tr>
+
        </tr>
−
                <tr>
+
        <tr>
−
                    <td><a href="http://parts.igem.org/Part:BBa_K2922010">BBa_K2922010</a></td>
+
            <td></td>
−
                    <td>112-kil</td>
+
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332048">BBa_K3332048</a></td>
−
                    <td>The expression of Kil protein<br>
+
            <td>Composite</td>
−
                        by J23112  promoter</td>
+
            <td>J23100-B0034-lpp-ompA-inap</td>
−
                    <td>Composite</td>
+
            <td>Shengyang Zhang</td>
−
                    <td>248</td>
+
            <td>1812</td>
−
                </tr>
+
        </tr>
−
                <tr>
+
        <tr>
−
                    <td><a href="http://parts.igem.org/Part:BBa_K2922011">BBa_K2922011</a></td>
+
            <td></td>
−
                    <td>114-kil</td>
+
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332049">BBa_K3332049</a></td>
−
                    <td>The expression of Kil protein<br>
+
            <td>Composite</td>
−
                        by J23114  promoter</td>
+
            <td>J23100-B0034-inap-aida-B0015</td>
−
                    <td>Composite</td>
+
            <td>Shengyang Zhang</td>
−
                    <td>248</td>
+
            <td>2829</td>
−
                </tr>
+
        </tr>
−
                <tr>
+
        <tr>
−
                    <td><a href="http://parts.igem.org/Part:BBa_K2922012">BBa_K2922012</a></td>
+
            <td></td>
−
                    <td>109-kil-T7-bgl1A</td>
+
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332050">BBa_K3332050</a></td>
−
                    <td>The secretion of <br>beta-glucosidase<br>
+
            <td>Composite</td>
−
                        by <a href="http://parts.igem.org/Part:BBa_K2922009">BBa_K2922009</a>.</td>
+
            <td>J23100-B0034-inap-brkA</td>
−
                    <td>Composite</td>
+
            <td>Shengyang Zhang</td>
−
                    <td>1760</td>
+
            <td>2745</td>
−
                </tr>
+
        </tr>
−
                <tr>
+
        <tr>
−
                    <td><a href="http://parts.igem.org/Part:BBa_K2922013">BBa_K2922013</a></td>
+
            <td></td>
−
                    <td>112-kil-T7-bgl1A</td>
+
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332051">BBa_K3332051</a></td>
−
                    <td>The secretion of <br>beta-glucosidase<br>
+
            <td>Composite</td>
−
                        by <a href="http://parts.igem.org/Part:BBa_K2922010">BBa_K2922010</a>.</td>
+
            <td>J23100-B0034-gox-his-tag-B0015</td>
−
                    <td>Composite</td>
+
            <td>Yangqi Deng</td>
−
                    <td>1760</td>
+
            <td>1149</td>
−
                </tr>
+
        </tr>
−
                <tr>
+
−
                    <td><a href="http://parts.igem.org/Part:BBa_K2922014">BBa_K2922014</a></td>
+
−
                    <td>114-kil-T7-bgl1A</td>
+
−
                    <td>The secretion of <br>beta-glucosidase<br>
+
−
                        by <a href="http://parts.igem.org/Part:BBa_K2922011">BBa_K2922011</a>.</td>
+
−
                    <td>Composite</td>
+
−
                    <td>1760</td>
+
−
                </tr>
+
−
                <tr>
+
−
                    <td><a href="http://parts.igem.org/Part:BBa_K2922015">BBa_K2922015</a></td>
+
−
                    <td>109-kil-T7-cenA</td>
+
−
                    <td>The secretion of endoglucanase<br>
+
−
                        by <a href="http://parts.igem.org/Part:BBa_K2922009">BBa_K2922009</a>.</td>
+
−
                    <td>Composite</td>
+
−
                    <td>1727</td>
+
−
                </tr>
+
−
                <tr>
+
−
                    <td><a href="http://parts.igem.org/Part:BBa_K2922016">BBa_K2922016</a></td>
+
−
                    <td>112-kil-T7-cenA</td>
+
−
                    <td>The secretion of endoglucanase<br>
+
−
                        by <a href="http://parts.igem.org/Part:BBa_K2922010">BBa_K2922010</a>.</td>
+
−
                    <td>Composite</td>
+
−
                    <td>1727</td>
+
−
                </tr>
+
−
                <tr>
+
−
                    <td><a href="http://parts.igem.org/Part:BBa_K2922017">BBa_K2922017</a></td>
+
−
                    <td>114-kil-T7-cenA</td>
+
−
                    <td>The secretion of endoglucanase<br>
+
−
                        by <a href="http://parts.igem.org/Part:BBa_K2922011">BBa_K2922011</a>.</td>
+
−
                    <td>Composite</td>
+
−
                    <td>1727</td>
+
−
                </tr>
+
−
                <tr>
+
−
                    <td><a href="http://parts.igem.org/Part:BBa_K2922018">BBa_K2922018</a></td>
+
−
                    <td>109-kil-T7-cex</td>
+
−
                    <td>The secretion of exoglucanase<br>
+
−
                        by <a href="http://parts.igem.org/Part:BBa_K2922009">BBa_K2922009</a>.</td>
+
−
                    <td>Composite</td>
+
−
                    <td>1835</td>
+
−
                </tr>
+
−
                <tr>
+
−
                    <td><a href="http://parts.igem.org/Part:BBa_K2922019">BBa_K2922019</a></td>
+
−
                    <td>112-kil-T7-cex</td>
+
−
                    <td>The secretion of exoglucanase<br>
+
−
                        by <a href="http://parts.igem.org/Part:BBa_K2922010">BBa_K2922010</a>.</td>
+
−
                    <td>Composite</td>
+
−
                    <td>1835</td>
+
−
                </tr>
+
  
−
                <tr>
+
        <tr>
−
                    <td><a href="http://parts.igem.org/Part:BBa_K2922020">BBa_K2922020</a></td>
+
            <td></td>
−
                    <td>114-kil-T7-cex</td>
+
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332052">BBa_K3332052</a></td>
−
                    <td>The secretion of exoglucanase<br>
+
            <td>Composite</td>
−
                        by <a href="http://parts.igem.org/Part:BBa_K2922011">BBa_K2922011</a>.</td>
+
            <td>J23100-B0034-inpnc-gox</td>
−
                    <td>Composite</td>
+
            <td>Yangqi Deng</td>
−
                    <td>1835</td>
+
            <td>2106</td>
−
                </tr>
+
        </tr>
−
             </table>
+
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332053">BBa_K3332053</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-lpp-ompA-gox</td>
 +
            <td>Yangqi Deng</td>
 +
            <td>1596</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332054">BBa_K3332054</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-gox-aida-B0015</td>
 +
            <td>Yangqi Deng</td>
 +
            <td>2613</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332055">BBa_K3332055</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-gox-brkA</td>
 +
            <td>Yangqi Deng</td>
 +
            <td>2529</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332056">BBa_K3332056</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-grhpr-his-tag-B0015</td>
 +
            <td>Yangqi Deng</td>
 +
            <td>1203</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332060">BBa_K3332060</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-grhpr-brkA-B0015</td>
 +
            <td>Xiaoyu Wang</td>
 +
            <td>2583</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332058">BBa_K3332058</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-lpp-ompA-grhpr</td>
 +
            <td>Qingliu Wang</td>
 +
            <td>1650</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332059">BBa_K3332059</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-grhpr-aida</td>
 +
            <td>Qingliu Wang</td>
 +
            <td>2667</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332061">BBa_K3332061</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-inpnc-egfp-B0015</td>
 +
            <td>Qingliu Wang</td>
 +
            <td>1893</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332062">BBa_K3332062</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-lpp-ompA-egfp</td>
 +
            <td>Qingliu Wang</td>
 +
            <td>1383</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332063">BBa_K3332063</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-egfp-aida-B0015</td>
 +
            <td>Fude Chen</td>
 +
            <td>2397</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332064">BBa_K3332064</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-egfp-brkA-B0015</td>
 +
            <td>Yuan Li</td>
 +
            <td>2450</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332065">BBa_K3332065</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-phnE<sub>1</sub></td>
 +
            <td>Gezhi Xiao</td>
 +
            <td>1024</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332066">BBa_K3332066</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-phnE<sub>2</sub></td>
 +
            <td>Meihao Ou</td>
 +
            <td>1579</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332067">BBa_K3332067</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-phnE<sub>1</sub>-B0034-phnE<sub>2</sub></td>
 +
            <td>Yuan Li</td>
 +
            <td>2568</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332068">BBa_K3332068</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-phnJ</td>
 +
            <td>Tianyu Feng</td>
 +
            <td>907</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332069">BBa_K3332069</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-phnJ_mut21</td>
 +
            <td>Siru Zhou</td>
 +
            <td>907</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332070">BBa_K3332070</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-phnJ_mut40&16</td>
 +
            <td>Siru Zhou</td>
 +
            <td>907</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332071">BBa_K3332071</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-phnJ_mut45</td>
 +
            <td>Siru Zhou</td>
 +
            <td>907</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332072">BBa_K3332072</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-phnO-his-tag</td>
 +
            <td>Siru Zhou</td>
 +
            <td>681</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332073">BBa_K3332073</a></td>
 +
            <td>Composite</td>
 +
            <td>J23101-ompA 5'UTR-phnF 0.97-hfp binding sequence</td>
 +
            <td>Siru Zhou</td>
 +
            <td>286</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332074">BBa_K3332074</a></td>
 +
            <td>Composite</td>
 +
            <td>J23101-ompA 5'UTR-phnF 0.85-hfp binding sequence</td>
 +
            <td>Siru Zhou</td>
 +
            <td>286</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332075">BBa_K3332075</a></td>
 +
            <td>Composite</td>
 +
            <td>J23101-ompA 5'UTR-phnF 0.75-hfp binding sequence</td>
 +
            <td>Meihao Ou</td>
 +
            <td>286</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332076">BBa_K3332076</a></td>
 +
            <td>Composite</td>
 +
            <td>J23101-ompA 5'UTR-phnJ 0.94-hfp binding sequence</td>
 +
            <td>Meihao Ou</td>
 +
            <td>286</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332077">BBa_K3332077</a></td>
 +
            <td>Composite</td>
 +
            <td>J23101-ompA 5'UTR-phnJ 0.69-hfp binding sequence</td>
 +
            <td>Meihao Ou</td>
 +
            <td>286</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332078">BBa_K3332078</a></td>
 +
            <td>Composite</td>
 +
            <td>Formaldehyde promoter-Inverter-MazF</td>
 +
            <td>Meihao Ou</td>
 +
            <td>2072</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332079">BBa_K3332079</a></td>
 +
            <td>Composite</td>
 +
            <td>pBAD/araC promoter-Inverter-EYFP</td>
 +
            <td>Meihao Ou</td>
 +
            <td>2476</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332080">BBa_K3332080</a></td>
 +
            <td>Composite</td>
 +
            <td>pBAD/araC promoter-cI repressor</td>
 +
            <td>Meihao Ou</td>
 +
            <td>2148</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332081">BBa_K3332081</a></td>
 +
            <td>Composite</td>
 +
            <td>pBAD/araC promoter-Inverter-MazF</td>
 +
            <td>Shichen Geng</td>
 +
            <td>2687</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332082">BBa_K3332082</a></td>
 +
            <td>Composite</td>
 +
            <td>pBAD/araC promoter-B0034-EYFP</td>
 +
            <td>Shichen Geng</td>
 +
            <td>2096</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332083">BBa_K3332083</a></td>
 +
            <td>Composite</td>
 +
            <td>pBAD/araC promoter-B0034-MazF</td>
 +
            <td>Shichen Geng</td>
 +
            <td>1712</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332084">BBa_K3332084</a></td>
 +
            <td>Composite</td>
 +
            <td>pLtetO-1-B0034-ECFP-B0015</td>
 +
            <td>Shichen Geng</td>
 +
            <td>1028</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332085">BBa_K3332085</a></td>
 +
            <td>Composite</td>
 +
            <td>J23106-B0031-tetR-B0015-pLtetO-1-ECFP-B0015</td>
 +
            <td>Shichen Geng</td>
 +
            <td>1921</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332086">BBa_K3332086</a></td>
 +
            <td>Composite</td>
 +
            <td>pTrc-2 promoter-B0034-ECFP-B0015</td>
 +
            <td>Shichen Geng</td>
 +
            <td>977</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332087">BBa_K3332087</a></td>
 +
            <td>Composite</td>
 +
            <td>pTrc-2 derivative promoter-B0034-ECFP-B0015</td>
 +
            <td>Ruijie Mo</td>
 +
            <td>1016</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332088">BBa_K3332088</a></td>
 +
            <td>Composite</td>
 +
            <td>pLtetO-1 promoter-RBS1-LacI-ssrAtag(mf-lon)-B0015-pTrc-2<br> promoter-B0034-ECFP-B0015</td>
 +
            <td>Ruijie Mo</td>
 +
            <td>2472</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332089">BBa_K3332089</a></td>
 +
            <td>Composite</td>
 +
            <td>pLtetO-1 promoter_RBS1-LacI-ssrAtag(mf-lon)-B0015-pTrc-2 derivative promoter-B0034-ECFP-B0015</td>
 +
            <td>Ruijie Mo</td>
 +
            <td>2511</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332090">BBa_K3332090</a></td>
 +
            <td>Composite</td>
 +
            <td>Formaldehyde promoter-B0034-ECFP</td>
 +
            <td>Ruijie Mo</td>
 +
            <td>1481</td>
 +
        </tr>
 +
        <tr>
 +
             <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332091">BBa_K3332091</a></td>
 +
            <td>Composite</td>
 +
            <td>Formaldehyde_derivative-1 promoter-ECFP</td>
 +
            <td>Ruijie Mo</td>
 +
            <td>1467</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332092">BBa_K3332092</a></td>
 +
            <td>Composite</td>
 +
            <td>Formaldehyde_derivative-3 promoter-ECFP</td>
 +
            <td>Shiyi Zhai</td>
 +
            <td>1517</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332093">BBa_K3332093</a></td>
 +
            <td>Composite</td>
 +
            <td>Formaldehyde_derivative-2 promoter-ECFP</td>
 +
            <td>Ruijie Mo</td>
 +
            <td>1531</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332097">BBa_K3332097</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-phnJ-B0015-J23100-B0034-phnE<sub>1</sub>E<sub>2</sub></td>
 +
            <td>Shiyi Zhai</td>
 +
            <td>3620</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332098">BBa_K3332098</a></td>
 +
            <td>Composite</td>
 +
            <td>RNAi sequence of phnF(0.97)-RNAi sequence of phnJ(0.94)</td>
 +
            <td>Shiyi Zhai</td>
 +
            <td>494</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332099">BBa_K3332099</a></td>
 +
            <td>Composite</td>
 +
            <td>RNAi sequence of phnF(0.97)-RNAi sequence of phnJ(0.69)</td>
 +
            <td>Shiyi Zhai</td>
 +
            <td>494</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332100">BBa_K3332100</a></td>
 +
            <td>Composite</td>
 +
            <td>J23101-RNAi sequence of phnF(0.97)-J61048-J23101-RNAi sequence of phnJ(0.69)-J61048-J23100-B0034-phnJ-J23100-B0034-phnO-J23100-B0034-phnE<sub>1</sub>E<sub>2</sub></td>
 +
            <td>Shiyi Zhai</td>
 +
            <td>4674</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332101">BBa_K3332101</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-phnJ_mut21-B0015-J23100-B0034-phnE<sub>1</sub>E<sub>2</sub></td>
 +
            <td>Meihao Ou</td>
 +
            <td>3620</td>
 +
        </tr>
 +
        <tr>
 +
            <td></td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332102">BBa_K3332102</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-phnJ_mut16&40-B0015-J23100-B0034-phnE<sub>1</sub>E<sub>2</sub></td>
 +
            <td>Meihao Ou</td>
 +
            <td>3620</td>
 +
        </tr>
 +
    </table>
 +
</div>
 +
    </section>
 +
    <section id="PC" class="js-scroll-step">
 +
        <div class="headline">
 +
            Part Collection
 
         </div>
 
         </div>
 +
        <p class="indent" style="margin-bottom:0;">This year, we have formulated a Part Collection related to surface display systems, which includes four kinds of anchor proteins: INPNC, AIDA, BrkA and Lpp-OmpA. The cleaved passenger domain remains adhered on the bacterial surface, which is conducive to keep the displayed exogenous proteins associated onto the cell, thus, the functionalized bacteria can be recycled/re-used conveniently.
 +
        </p>
 +
<p class="indent" style="margin-bottom:0;">Users can also combine the anchor proteins with different proteins to verify the function of the anchored proteins and treat it as a nice way to avoid the influence of components inside the cell. For our Part Collection, we’ve characterized three different anchored proteins combined with eGFP, GOX, GRHPR and iNap separately to test its efficacy and uploaded complete and detailed quantitative data to the registry, which is believed to be very instructive for the design and characterization of many teams.</p>
 +
        <br>
 +
        <div class="table_container">
 +
    <table class="table">
 +
        <tr>
 +
            <th>Anchor Protein</th>
 +
            <th>Fusion</th>
 +
            <th>Name</th>
 +
            <th>Type</th>
 +
            <th>Description</th>
 +
            <th>Designer</th>
 +
            <th>Length</th>
 +
        </tr>
 +
        <tr>
 +
            <td rowspan="8">inpnc</td>
 +
            <td rowspan="8">C-terminal</td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332016">BBa_K3332016</a></td>
 +
            <td>Coding</td>
 +
            <td>inpnc-egfp</td>
 +
            <td>Shi Zhang</td>
 +
            <td>1695</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332002">BBa_K3332002</a></td>
 +
            <td>Coding</td>
 +
            <td>inpnc-inap</td>
 +
            <td>Shuling Xiao</td>
 +
            <td>2128</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332007">BBa_K3332007</a></td>
 +
            <td>Coding</td>
 +
            <td>inpnc-gox</td>
 +
            <td>Xiaoyu Wang</td>
 +
            <td>1908</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332012">BBa_K3332012</a></td>
 +
            <td>Coding</td>
 +
            <td>inpnc-grhpr</td>
 +
            <td>Shi Zhang</td>
 +
            <td>1962</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332061">BBa_K3332061</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-inpnc-egfp-B0015</td>
 +
            <td>Qingliu Wang</td>
 +
            <td>1893</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332047">BBa_K3332047</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-inpnc-inap-B0015</td>
 +
            <td>Shengyang Zhang</td>
 +
            <td>2328</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332052">BBa_K3332052</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-inpnc-gox-B0015</td>
 +
            <td>Yangqi Deng</td>
 +
            <td>2106</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332057">BBa_K3332057</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-inpnc-grhpr-B0015</td>
 +
            <td>Qingliu Wang</td>
 +
            <td>2160</td>
 +
        </tr>
 +
        <tr>
 +
            <td rowspan="8">aida</td>
 +
            <td rowspan="8">N-terminal</td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332018">BBa_K3332018</a></td>
 +
            <td>Coding</td>
 +
            <td>egfp-aida</td>
 +
            <td>Yixian Zheng</td>
 +
            <td>2199</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332004">BBa_K3332004</a></td>
 +
            <td>Coding</td>
 +
            <td>inap-aida</td>
 +
            <td>Shuling Xiao</td>
 +
            <td>2631</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332009">BBa_K3332009</a></td>
 +
            <td>Coding</td>
 +
            <td>gox-aida</td>
 +
            <td>Xiaoyu Wang</td>
 +
            <td>2415</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332014">BBa_K3332014</a></td>
 +
            <td>Coding</td>
 +
            <td>grhpr-aida</td>
 +
            <td>Shi Zhang</td>
 +
            <td>2469</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332063">BBa_K3332063</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-egfp-aida-B0015</td>
 +
            <td>Fude Chen</td>
 +
            <td>2397</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332049">BBa_K3332049</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-inap-aida-B0015</td>
 +
            <td>Shengyang Zhang</td>
 +
            <td>2829</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332054">BBa_K3332054</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-gox-aida-B0015</td>
 +
            <td>Yangqi Deng</td>
 +
            <td>2613</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332059">BBa_K3332059</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-grhpr-aida-B0015</td>
 +
            <td>Qingliu Wang</td>
 +
            <td>2667</td>
 +
        </tr>
 +
        <tr>
 +
            <td rowspan="8">brkA</td>
 +
            <td rowspan="8">N-terminal</td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332019">BBa_K3332019</a></td>
 +
            <td>Coding</td>
 +
            <td>egfp-brkA</td>
 +
            <td>Yixian Zheng</td>
 +
            <td>2252</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332005">BBa_K3332005</a></td>
 +
            <td>Coding</td>
 +
            <td>inap-brkA</td>
 +
            <td>Shuling Xiao</td>
 +
            <td>2574</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332010">BBa_K3332010</a></td>
 +
            <td>Coding</td>
 +
            <td>gox-brkA</td>
 +
            <td>Xiaoyu Wang</td>
 +
            <td>2331</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332015">BBa_K3332015</a></td>
 +
            <td>Coding</td>
 +
            <td>grhpr-brkA</td>
 +
            <td>Shi Zhang</td>
 +
            <td>2385</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332064">BBa_K3332064</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-egfp-brkA-B0015</td>
 +
            <td>Fude Chen</td>
 +
            <td>2450</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332050">BBa_K3332050</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-inap-brkA-B0015</td>
 +
            <td>Shengyang Zhang</td>
 +
            <td>2745</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332055">BBa_K3332055</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-gox-brkA-B0015</td>
 +
            <td>Yangqi Deng</td>
 +
            <td>2529</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332060">BBa_K3332060</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-grhpr-brkA-B0015</td>
 +
            <td>Qingliu Wang</td>
 +
            <td>2583</td>
 +
        </tr>
 +
        <tr>
 +
            <td rowspan="8">lpp-ompA</td>
 +
            <td rowspan="8">C-terminal</td>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332017">BBa_K3332017</a></td>
 +
            <td>Coding</td>
 +
            <td>lpp-ompA-egfp</td>
 +
            <td>Shi Zhang</td>
 +
            <td>1185</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332003">BBa_K3332003</a></td>
 +
            <td>Coding</td>
 +
            <td>lpp-ompA-inap</td>
 +
            <td>Shuling Xiao</td>
 +
            <td>1614</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332008">BBa_K3332008</a></td>
 +
            <td>Coding</td>
 +
            <td>lpp-ompA-gox</td>
 +
            <td>Xiaoyu Wang</td>
 +
            <td>1398</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332013">BBa_K3332013</a></td>
 +
            <td>Coding</td>
 +
            <td>lpp-ompA-grhpr</td>
 +
            <td>Shi Zhang</td>
 +
            <td>1452</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332062">BBa_K3332062</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-lpp-ompA-egfp-B0015</td>
 +
            <td>Qingliu Wang</td>
 +
            <td>1383</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332048">BBa_K3332048</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-lpp-ompA-inap-B0015</td>
 +
            <td>Shengyang Zhang</td>
 +
            <td>1812</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332053">BBa_K3332053</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-lpp-ompA-gox-B0015</td>
 +
            <td>Yangqi Deng</td>
 +
            <td>1596</td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3332058">BBa_K3332058</a></td>
 +
            <td>Composite</td>
 +
            <td>J23100-B0034-lpp-ompA-grhpr-B0015</td>
 +
            <td>Qingliu Wang</td>
 +
            <td>1650</td>
 +
        </tr>
 +
    </table>
 +
</div>
 
     </section>
 
     </section>
 
</div>
 
</div>

Latest revision as of 05:26, 21 December 2020


This year, among the 103 parts designed, 44 of them are basic parts and the rest are composite parts. All of the parts are designed by ourselves, and most of them have been tested to insure their reliability. You can click here to learn more about our project to see their specific function, or click on the part numbers when browse website ( parts.igem.org ).

Basic Parts

Among the 44 basic parts designed this year by ourselves, BBa_K3332011 is our favorite one, which comes from homo liver. The gene enables engineering bacteria to efficiently catalyze the reaction of reducing glyoxalic acid and consuming NADPH. Besides, many other basic parts have also been designed to construct our project.


Favorite Name Type Description Designer Length
R BBa_K3332011 Coding grhpr-his-tag Xiaoyu Wang 1005
BBa_K3332000 Coding inap-his-tag Shuling Xiao 1167
BBa_K3332001 Coding his-tag-inap Shuling Xiao 1167
BBa_K3332002 Coding inpnc-inap Shuling Xiao 2128
BBa_K3332003 Coding lpp-ompA-inap Shuling Xiao 1614
BBa_K3332004 Coding inap-aida Shuling Xiao 2631
BBa_K3332005 Coding inap-brkA Shuling Xiao 2547
BBa_K3332006 Coding gox-his-tag Xiaoyu Wang 951
BBa_K3332007 Coding inpnc-gox Xiaoyu Wang 1908
BBa_K3332008 Coding lpp-ompA-gox Xiaoyu Wang 1398
BBa_K3332009 Coding gox-aida Xiaoyu Wang 2415
BBa_K3332010 Coding gox-brkA Xiaoyu Wang 2331
BBa_K3332012 Coding inpnc-grhpr Shi Zhang 1962
BBa_K3332013 Coding lpp-ompA-grhpr Shi Zhang 1452
BBa_K3332014 Coding grhpr-aida Shi Zhang 2469
BBa_K3332015 Coding grhpr-brkA Shi Zhang 2385
BBa_K3332016 Coding inpnc-egfp Shi Zhang 1695
BBa_K3332017 Coding lpp-ompA-egfp Shi Zhang 1185
BBa_K3332018 Coding egfp-aida Yixian Zheng 2199
BBa_K3332019 Coding egfp-brkA Yixian Zheng 2252
BBa_K3332020 Coding phnE1 Yixian Zheng 963
BBa_K3332021 Coding phnE2 Yixian Zheng 1518
BBa_K3332022 Coding phnC Yixian Zheng 840
BBa_K3332023 Coding phnD Yixian Zheng 906
BBa_K3332024 Coding phnJ Zhichun Yang 846
BBa_K3332025 Coding phnJ_mut21 Zhichun Yang 846
BBa_K3332026 Coding phnJ_mut40&16 Zhichun Yang 846
BBa_K3332027 Coding phnJ_mut45 Zhichun Yang 846
BBa_K3332028 Coding phnO-his-tag Jisheng Xie 483
BBa_K3332029 DNA ompA 5'UTR-phnF 0.97-hfp binding sequence Zhichun Yang 223
BBa_K3332030 DNA ompA 5'UTR-phnF 0.85-hfp binding sequence Ruomeng Bi 223
BBa_K3332031 DNA ompA 5'UTR-phnF 0.75-hfp binding sequence Ruomeng Bi 223
BBa_K3332032 DNA ompA 5'UTR-phnJ 0.94-hfp binding sequence Ruomeng Bi 223
BBa_K3332033 DNA ompA 5'UTR-phnJ 0.69-hfp binding sequence Ruomeng Bi 223
BBa_K3332034 DNA pLtetO-1 promoter Ruomeng Bi 142
BBa_K3332035 Coding E0020-ssrAtag（mf-lon） Ruomeng Bi 819
BBa_K3332036 RBS RBS1 Jinzhu Mao 9
BBa_K3332037 Coding LacI-ssrAtag(mf-lon) Jinzhu Mao 1185
BBa_K3332038 DNA pTrc-2 promoter Jinzhu Mao 91
BBa_K3332039 Coding tetR Jinzhu Mao 624
BBa_K3332040 DNA pTrc-2 derivative promoter Ruomeng Bi 130
BBa_K3332042 DNA Formaldehyde_derivative-1 promoter Jisheng Xie 581
BBa_K3332043 DNA Formaldehyde_derivative-2 promoter Zinuo Huang 645
BBa_K3332044 DNA Formaldehyde_derivative-3 promoter Xingyu Chen 631
Composite Parts

A series of composite parts have been designed this year, and most of them are useful for our project. And our favorite composite part is BBa_K3332057 , which contains the sequence of the anchor protein—INPNC to anchor our favorite basic part—GRHPR.

The anchor protein is from a kind of autotransporter family which can be anchored to the membrane and work as a bacterial display system. The cleaved passenger domain remains adhered on the bacterial surface and it is conducive to keep the displayed exogenous proteins associated onto the cell. Thus, the functionalized bacteria can be recycled/re-used conveniently.

Throughout the season we’ve combined several surface display systems with GRHPR and verified the function of detecting NADPH outside the cell. After a series of experiments, INPNC is determined as the most suitable anchor protein for GRHPR.


Favorite Name Type Description Designer Length
R BBa_K3332057 Composite J23100-B0034-inpnc-grhpr Qingliu Wang 2160
BBa_K3332045 Composite J23100-B0034-inap-his-tag-B0015 Shengyang Zhang 1365
BBa_K3332046 Composite J23100-B0034-his-tag-inap-B0015 Shengyang Zhang 1365
BBa_K3332047 Composite J23100-B0034-inpnc-inap Shengyang Zhang 2328
BBa_K3332048 Composite J23100-B0034-lpp-ompA-inap Shengyang Zhang 1812
BBa_K3332049 Composite J23100-B0034-inap-aida-B0015 Shengyang Zhang 2829
BBa_K3332050 Composite J23100-B0034-inap-brkA Shengyang Zhang 2745
BBa_K3332051 Composite J23100-B0034-gox-his-tag-B0015 Yangqi Deng 1149
BBa_K3332052 Composite J23100-B0034-inpnc-gox Yangqi Deng 2106
BBa_K3332053 Composite J23100-B0034-lpp-ompA-gox Yangqi Deng 1596
BBa_K3332054 Composite J23100-B0034-gox-aida-B0015 Yangqi Deng 2613
BBa_K3332055 Composite J23100-B0034-gox-brkA Yangqi Deng 2529
BBa_K3332056 Composite J23100-B0034-grhpr-his-tag-B0015 Yangqi Deng 1203
BBa_K3332060 Composite J23100-B0034-grhpr-brkA-B0015 Xiaoyu Wang 2583
BBa_K3332058 Composite J23100-B0034-lpp-ompA-grhpr Qingliu Wang 1650
BBa_K3332059 Composite J23100-B0034-grhpr-aida Qingliu Wang 2667
BBa_K3332061 Composite J23100-B0034-inpnc-egfp-B0015 Qingliu Wang 1893
BBa_K3332062 Composite J23100-B0034-lpp-ompA-egfp Qingliu Wang 1383
BBa_K3332063 Composite J23100-B0034-egfp-aida-B0015 Fude Chen 2397
BBa_K3332064 Composite J23100-B0034-egfp-brkA-B0015 Yuan Li 2450
BBa_K3332065 Composite J23100-B0034-phnE1 Gezhi Xiao 1024
BBa_K3332066 Composite J23100-B0034-phnE2 Meihao Ou 1579
BBa_K3332067 Composite J23100-B0034-phnE1-B0034-phnE2 Yuan Li 2568
BBa_K3332068 Composite J23100-B0034-phnJ Tianyu Feng 907
BBa_K3332069 Composite J23100-B0034-phnJ_mut21 Siru Zhou 907
BBa_K3332070 Composite J23100-B0034-phnJ_mut40&16 Siru Zhou 907
BBa_K3332071 Composite J23100-B0034-phnJ_mut45 Siru Zhou 907
BBa_K3332072 Composite J23100-B0034-phnO-his-tag Siru Zhou 681
BBa_K3332073 Composite J23101-ompA 5'UTR-phnF 0.97-hfp binding sequence Siru Zhou 286
BBa_K3332074 Composite J23101-ompA 5'UTR-phnF 0.85-hfp binding sequence Siru Zhou 286
BBa_K3332075 Composite J23101-ompA 5'UTR-phnF 0.75-hfp binding sequence Meihao Ou 286
BBa_K3332076 Composite J23101-ompA 5'UTR-phnJ 0.94-hfp binding sequence Meihao Ou 286
BBa_K3332077 Composite J23101-ompA 5'UTR-phnJ 0.69-hfp binding sequence Meihao Ou 286
BBa_K3332078 Composite Formaldehyde promoter-Inverter-MazF Meihao Ou 2072
BBa_K3332079 Composite pBAD/araC promoter-Inverter-EYFP Meihao Ou 2476
BBa_K3332080 Composite pBAD/araC promoter-cI repressor Meihao Ou 2148
BBa_K3332081 Composite pBAD/araC promoter-Inverter-MazF Shichen Geng 2687
BBa_K3332082 Composite pBAD/araC promoter-B0034-EYFP Shichen Geng 2096
BBa_K3332083 Composite pBAD/araC promoter-B0034-MazF Shichen Geng 1712
BBa_K3332084 Composite pLtetO-1-B0034-ECFP-B0015 Shichen Geng 1028
BBa_K3332085 Composite J23106-B0031-tetR-B0015-pLtetO-1-ECFP-B0015 Shichen Geng 1921
BBa_K3332086 Composite pTrc-2 promoter-B0034-ECFP-B0015 Shichen Geng 977
BBa_K3332087 Composite pTrc-2 derivative promoter-B0034-ECFP-B0015 Ruijie Mo 1016
BBa_K3332088 Composite pLtetO-1 promoter-RBS1-LacI-ssrAtag(mf-lon)-B0015-pTrc-2
promoter-B0034-ECFP-B0015		 Ruijie Mo 2472
BBa_K3332089 Composite pLtetO-1 promoter_RBS1-LacI-ssrAtag(mf-lon)-B0015-pTrc-2 derivative promoter-B0034-ECFP-B0015 Ruijie Mo 2511
BBa_K3332090 Composite Formaldehyde promoter-B0034-ECFP Ruijie Mo 1481
BBa_K3332091 Composite Formaldehyde_derivative-1 promoter-ECFP Ruijie Mo 1467
BBa_K3332092 Composite Formaldehyde_derivative-3 promoter-ECFP Shiyi Zhai 1517
BBa_K3332093 Composite Formaldehyde_derivative-2 promoter-ECFP Ruijie Mo 1531
BBa_K3332097 Composite J23100-B0034-phnJ-B0015-J23100-B0034-phnE1E2 Shiyi Zhai 3620
BBa_K3332098 Composite RNAi sequence of phnF(0.97)-RNAi sequence of phnJ(0.94) Shiyi Zhai 494
BBa_K3332099 Composite RNAi sequence of phnF(0.97)-RNAi sequence of phnJ(0.69) Shiyi Zhai 494
BBa_K3332100 Composite J23101-RNAi sequence of phnF(0.97)-J61048-J23101-RNAi sequence of phnJ(0.69)-J61048-J23100-B0034-phnJ-J23100-B0034-phnO-J23100-B0034-phnE1E2 Shiyi Zhai 4674
BBa_K3332101 Composite J23100-B0034-phnJ_mut21-B0015-J23100-B0034-phnE1E2 Meihao Ou 3620
BBa_K3332102 Composite J23100-B0034-phnJ_mut16&40-B0015-J23100-B0034-phnE1E2 Meihao Ou 3620
Part Collection

This year, we have formulated a Part Collection related to surface display systems, which includes four kinds of anchor proteins: INPNC, AIDA, BrkA and Lpp-OmpA. The cleaved passenger domain remains adhered on the bacterial surface, which is conducive to keep the displayed exogenous proteins associated onto the cell, thus, the functionalized bacteria can be recycled/re-used conveniently.

Users can also combine the anchor proteins with different proteins to verify the function of the anchored proteins and treat it as a nice way to avoid the influence of components inside the cell. For our Part Collection, we’ve characterized three different anchored proteins combined with eGFP, GOX, GRHPR and iNap separately to test its efficacy and uploaded complete and detailed quantitative data to the registry, which is believed to be very instructive for the design and characterization of many teams.


Anchor Protein Fusion Name Type Description Designer Length
inpnc C-terminal BBa_K3332016 Coding inpnc-egfp Shi Zhang 1695
BBa_K3332002 Coding inpnc-inap Shuling Xiao 2128
BBa_K3332007 Coding inpnc-gox Xiaoyu Wang 1908
BBa_K3332012 Coding inpnc-grhpr Shi Zhang 1962
BBa_K3332061 Composite J23100-B0034-inpnc-egfp-B0015 Qingliu Wang 1893
BBa_K3332047 Composite J23100-B0034-inpnc-inap-B0015 Shengyang Zhang 2328
BBa_K3332052 Composite J23100-B0034-inpnc-gox-B0015 Yangqi Deng 2106
BBa_K3332057 Composite J23100-B0034-inpnc-grhpr-B0015 Qingliu Wang 2160
aida N-terminal BBa_K3332018 Coding egfp-aida Yixian Zheng 2199
BBa_K3332004 Coding inap-aida Shuling Xiao 2631
BBa_K3332009 Coding gox-aida Xiaoyu Wang 2415
BBa_K3332014 Coding grhpr-aida Shi Zhang 2469
BBa_K3332063 Composite J23100-B0034-egfp-aida-B0015 Fude Chen 2397
BBa_K3332049 Composite J23100-B0034-inap-aida-B0015 Shengyang Zhang 2829
BBa_K3332054 Composite J23100-B0034-gox-aida-B0015 Yangqi Deng 2613
BBa_K3332059 Composite J23100-B0034-grhpr-aida-B0015 Qingliu Wang 2667
brkA N-terminal BBa_K3332019 Coding egfp-brkA Yixian Zheng 2252
BBa_K3332005 Coding inap-brkA Shuling Xiao 2574
BBa_K3332010 Coding gox-brkA Xiaoyu Wang 2331
BBa_K3332015 Coding grhpr-brkA Shi Zhang 2385
BBa_K3332064 Composite J23100-B0034-egfp-brkA-B0015 Fude Chen 2450
BBa_K3332050 Composite J23100-B0034-inap-brkA-B0015 Shengyang Zhang 2745
BBa_K3332055 Composite J23100-B0034-gox-brkA-B0015 Yangqi Deng 2529
BBa_K3332060 Composite J23100-B0034-grhpr-brkA-B0015 Qingliu Wang 2583
lpp-ompA C-terminal BBa_K3332017 Coding lpp-ompA-egfp Shi Zhang 1185
BBa_K3332003 Coding lpp-ompA-inap Shuling Xiao 1614
BBa_K3332008 Coding lpp-ompA-gox Xiaoyu Wang 1398
BBa_K3332013 Coding lpp-ompA-grhpr Shi Zhang 1452
BBa_K3332062 Composite J23100-B0034-lpp-ompA-egfp-B0015 Qingliu Wang 1383
BBa_K3332048 Composite J23100-B0034-lpp-ompA-inap-B0015 Shengyang Zhang 1812
BBa_K3332053 Composite J23100-B0034-lpp-ompA-gox-B0015 Yangqi Deng 1596
BBa_K3332058 Composite J23100-B0034-lpp-ompA-grhpr-B0015 Qingliu Wang 1650

Xiamen University, Fujian, China

No.422, Siming South Road, Fujian, P.R.China 361005