Difference between revisions of "Team:TU Darmstadt/Project/Backbones"

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  <thead>
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          <table class="table table-light">
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    <tr>
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            <tr>
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      <th scope="col">Name</th>
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              <th scope="col">Name</th>
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      <th scope="col">Resistance</th>
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              <th scope="col">Resistance</th>
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      <th scope="col">Size</th>
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              <th scope="col">Size</th>
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      <th scope="col">Features</th>
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              <th scope="col">Features <b>This is a placeholder</b></th>
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    </tr>
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              <th scope="col">Used for the following parts</th>
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  </thead>
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            </tr>
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  <tbody>
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            <tr>
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    <tr>
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              <td>pACYCT2</td>
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      <th scope="row">1</th>
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              <td>Cmp</td>
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      <td>Mark</td>
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              <td>3975 bp</td>
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      <td>Otto</td>
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              <td>
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      <td>@mdo</td>
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                cat promoter, CmR, p15A ori, lacI repressor + lacI promoter, T7
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    </tr>
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                promoter, lac operator, T7 terminator
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    <tr>
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              </td>
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      <th scope="row">2</th>
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              <td><a href=" http://parts.igem.org/Part:BBa_K3187000 "
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      <td>Jacob</td>
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                    target="_blank"> BBa_K3187000</a>, <a href=" http://parts.igem.org/Part:BBa_K3187001 "
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      <td>Thornton</td>
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                    target="_blank"> BBa_K3187001</a>, <a href=" http://parts.igem.org/Part:BBa_K3187003"
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      <td>@fat</td>
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                    target="_blank"> BBa_K3187003</a>, <a href=" http://parts.igem.org/Part:BBa_K3187004" target="_blank"> BBa_K3187004</a>
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    </tr>
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</td>
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    <tr>
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            </tr>
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      <th scope="row">3</th>
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            <tr></tr>
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      <td colspan="2">Larry the Bird</td>
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            <tr>
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      <td>@twitter</td>
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              <td>pDESTara2</td>
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    </tr>
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              <td>Amp</td>
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  </tbody>
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              <td>4556 bp</td>
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</table>
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              <td>AmpR, araBAD promoter, araC, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, rrnB T1 and T7Te terminator
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                promoter, lac operator, T7 terminator</td>
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              <td><a href=" http://parts.igem.org/Part:BBa_K3187010 "
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                    target="_blank"> BBa_K3187010</a>
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</td>
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            </tr>
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            <tr>
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              <td>pET24</td>
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              <td>Kan</td>
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              <td>5236 bp</td>
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              <td>
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                KanR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, f1 ori, lacI
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                repressor + lacI promoter, T7 promoter + lac operator, 6xHis, T7
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                terminator
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              </td>
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              <td><a href=" http://parts.igem.org/Part:BBa_K3187000"
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                    target="_blank"> BBa_K3187000</a>, <a href=" http://parts.igem.org/Part:BBa_K3187002 "
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                    target="_blank"> BBa_K3187002</a>, <a href=" http://parts.igem.org/Part:BBa_K3187005 "
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                    target="_blank"> BBa_K3187005</a>, <a href=" http://parts.igem.org/Part:BBa_K3187006 "
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                    target="_blank"> BBa_K3187006</a>, <a href=" http://parts.igem.org/Part:BBa_K3187008 "
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                    target="_blank"> BBa_K3187008</a>
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</td>
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            </tr>
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            <tr>
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              <td>pSB1C3</td>
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              <td>Cmp</td>
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              <td>2070 bp</td>
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              <td>
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−
                CmR, lambda t0 terminator, high-copy-number
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                ColE1/ pMB1/ pBR322/ pUC ori, his operton Terminator, BioBrick Pre-
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                and Suffix
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              </td>
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              <td><a href=" http://parts.igem.org/Part:BBa_K3187014 "
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                    target="_blank"> BBa_K3187014</a>, <a href=" http://parts.igem.org/Part:BBa_K3187015 "
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                    target="_blank"> BBa_K3187015</a>
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</td>
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            </tr>
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            <tr>
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              <td>pTeTW3con2</td>
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              <td>Cmp</td>
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              <td>4555 bp</td>
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              <td>
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−
                cat promoter, CmR, p15A ori, lacI repressor + lacI promoter,
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                tetR/tetA promoter, tetR repressor, tet operator, T7 promoter +
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                lac operator, T7 terminator
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              </td>
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              <td><a href=" http://parts.igem.org/Part:BBa_K3187011"
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                    target="_blank"> BBa_K3187011</a>, <a href=" http://parts.igem.org/Part:BBa_K3187012"
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                    target="_blank"> BBa_K3187012</a>, <a href=" http://parts.igem.org/Part:BBa_K3187013"
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                    target="_blank"> BBa_K3187013</a>
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</td>
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            </tr>
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            <tr>
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−
           
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−
            </tr>
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          </table>
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        </div>
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      </div>
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Revision as of 14:08, 3 October 2020


Backbones

Backbones Table


University Experts Former iGEMers Others

Backbones

For the in silico design of our experiments for the wetlab we used several different plasmid backbones. Due to Covid-19 we were not able to test any of our constructs in the lab. Therefore, we only planned the shown backbones theoretically. The plasmids would be used for cloning, expression, and genomic integration of our constructs. You can find a list of all plasmids we used for our design and the corresponding parts below.
Name Resistance Size Features
1 Mark Otto @mdo
2 Jacob Thornton @fat
3 Larry the Bird @twitter