2020 Fudan Protocols
页面摘要,或者是单项奖核心点 Our project aims at converting engineered Our project aims at converting engineered Our project aims at converting engineered Our project aims at converting engi neered Our project aims at converting engineered Our project aims at converting engineered Our project aims at converting engineered Our project aims at converting engineered
Cell Cryopreservation
Cryopreservation is a technique that store cells at a very low temperatures (-80℃) to reduce cell metabolic damage and enable long-term storage.
- Add cryoprotectant: Add 80% glycerin 500μL and bacterial fluid 1 000μL into Cryopreservation vials. Mix upside down gently.
- Seal the vials: Stick sellotape around the label.
- Store the vials: Store prepared vials in refrigerator at -80℃.
Cell Recovery
Bacteria preserved at -80 ℃ need to be recovery to restores cell growth.
- Prepare plate: Take out the plate with relevant resistance from 4 degrees and wait for the temperature to the room temperature. Mark at the bottom of the plate.
- Recover the cell: The -80 ° C frozen strain was removed and placed on the ice quickly. Take a ring of the upper layer of melt bacteria liquid by the sterilized inoculation ring. Coated the plate and draw a line.
- Culture the recovered cell: Incubate at 37 degrees overnight./li>
PCR
PCR is performed for amplify DNA fragments in our project.
- Set up PCR system (25µl):
Reagent Volume ddH2O To 25 µl 2 x Phanta Max Buffer 12.5 µl dNTP 0.5 µl Phanta 0.5 µl Template
Plasmid: 0.5 µl
PCR product: 1µl
Forward Primer 1 µl Reverse Primer 1 µl - Place the PCR tubes in a PCR amplifier.
- Set up reaction program:
Procedure Temperature Time Cycle Initialization 95℃ 30s 1 Denaturation 95℃ 15s 40 Annealing 72℃ 15s Phanta 0.5 µl Template
Plasmid: 0.5 µl
PCR product: 1µl
Forward Primer 1 µl Reverse Primer 1 µl - Incubate: Incubate at 16℃ until the PCR product is picked up.
Cell Cryopreservation2
Cryopreservation is a technique that store cells at a very low temperatures (-80℃) to reduce cell metabolic damage and enable long-term storage.
- Add cryoprotectant: Add 80% glycerin 500μL and bacterial fluid 1 000μL into Cryopreservation vials. Mix upside down gently.
- Seal the vials: Stick sellotape around the label.
- The expression level of short peptides has a threshold, preventing any excessive calcium absorption that might lead to hypercalcemia 注意句子超长后的回折
- Store the vials: Store prepared vials in refrigerator at -80℃.
Cell Cryopreservation2
Cryopreservation is a technique that store cells at a very low temperatures (-80℃) to reduce cell metabolic damage and enable long-term storage.
- Add cryoprotectant: Add 80% glycerin 500μL and bacterial fluid 1 000μL into Cryopreservation vials. Mix upside down gently.
- Seal the vials: Stick sellotape around the label.
- The expression level of short peptides has a threshold, preventing any excessive calcium absorption that might lead to hypercalcemia 注意句子超长后的回折
- Store the vials: Store prepared vials in refrigerator at -80℃.
However, for the Chinese population, the bottlenecks are as follows:
- 注意这里列表的写法 页面字会变深 突出内容 ol是编数字的 nts
- calcium-supplementing pills daste
- The body's absorptioes as calcium intake increases
- Vegetables conta列表的写法 页面字会变深 突出内容 ol是编数字的 ul是没有标数字的列表
However, the currently popular calcium supplement method on the market is either to adjust diet or to consume a lot of calcium supplementing pills. For the elderly, they suffer from poor memory or neglect the importance of calcium pills, often forgetting to consume the supplement; for children, they often refuse to take calcium pills or to maintain some diet high in calcium, thus requiring the "liquid calcium" method to ensure sufficient calcium intake. Ho可以 继续大段内容
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章节内容的分段就用p开p关
章节内容的分段就用p开p关,额外需要关注的可以套一个highlight的框,细看 https://2020.igem.org/Resources/Template_Documentation#highlight
章节内容的分段就用p开p关
章节内容的分段就用p开p关 EXAM 外部链接 href尽量使用https
- 注意这里列表的写法 页面字会变深 突出内容 ul是没有标数字的列表 nts
- calcium-supple menting pills don’t haat taste
- Vegetables conta列表的写法 页面字会变深 突出内容 ol是编数字的 ul是没有标数字的列表
章节内容的分段就用p开p关
又来个章节 sis
引入文字 ion.
Firstly, the product is designed for oral use and our target user is the elderly. 英文分项不需要ol或ul
- The produc
- The short pep
- The expression level of short peptides has a threshold, preventing any excessive calcium absorption that might lead to hypercalcemia 注意句子超长后的回折
- There is a kill swit.
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Header 1 这是igem hq的表格的写法 | Header 2 |
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Content A 1 | Content B 1 |
Content A 2 | Content B 2 这是igem hq的表格的写法。表格都需要说明,之前之后都会有p来文字 |
Name | Item Name | Item Price |
---|---|---|
Alvin | Eclair | $0.87 |
Alan | Jellybean | $3.76 |
Jonathan | Lollipop | $7.00 |
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From the us 章节内分段 etition.
再来例子 Market Analysis
文本在左侧一半,宽屏 According to the Chinese Dietetic Sociietary Nutrient Intakes of Chinese Residents", the calcium requirement for infants and young children increases with age, from 300mg/day to 800mg/day, and the calcium requirement for adolescents reaches 1000mg/day. The amof the required intake, with the small exception of some coastal and pastoral areas 图片在右侧一半
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Content A 1 | Content B 1 | Content C 1 |
列融合 | Content C 2 这是igem hq的表格的写法。半幅或者2/3幅可以用。 行融合 就 用br了 |
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Signature: Jingqi