Line 138: | Line 138: | ||
<div class="containerimg" id=Chapter2> | <div class="containerimg" id=Chapter2> | ||
<figure> | <figure> | ||
− | <img src="https://static.igem.org/mediawiki/2020/6/6e/T--TU_Darmstadt--Composite_Filmies. | + | <img src="https://static.igem.org/mediawiki/2020/6/6e/https://static.igem.org/mediawiki/2020/1/1d/T--TU_Darmstadt--Composite_Filmies.png" width="100%"> |
<figcaption id="Figure#">Figure 2: Composite parts for immobilization on biofilm matrix </figcaption> | <figcaption id="Figure#">Figure 2: Composite parts for immobilization on biofilm matrix </figcaption> | ||
</figure> | </figure> |
Revision as of 19:07, 19 October 2020
Due to Covid-19 we were not able to test any of our designed parts in the lab. Nevertheless, we registered our basic parts, so future teams are easily able to characterize them. For our Composite Parts we only uploaded one for which we can provide modeling data. We refrained from uploading the others to the registry as there exists no primary literature on them, but we still provide schematic images on them.
Basic Parts
Due to Covid-19 we were not able to test any of our designed parts in the lab. Nevertheless, we registered our basic parts, so future teams are easily able to characterize them. For our Composite Parts we refrained from uploading them to the registry as there exists no primary literature on them, but we still provide schematic images on them.
All our basic parts are depicted in the table below. Because we were not able to conduct any lab work , we did not submit any parts for the special prizes Best Basic Part.
Part(BBa_) | Type | Description | Length in bp |
---|---|---|---|
BBa_K3429000 | Promotor | pgrac promotor | 170 |
BBa_K3429001 | Coding Sequence | TasA matrix protein | 783 |
BBa_K3429002 | Coding Sequence | superfolder Green Fluorescent Protein (sfGFP) | 712 |
BBa_K3429003 | Coding Sequence | Protein Linker for fusion proteins in B. subtilis: ARGGGGSGGGGSGS | 42 |
BBa_K3429004 | Terminator | trpA-terminator | 24 |
BBa_K3429005 | Coding Sequence | Ribosomal Protein S2 (rpsB) from B. subtilis | 721 |
BBa_K3429006 | Promotor | Quorum sensing promotor with 3 binding sites (ComA, DegU, Sig A) from B. subtilis. | 192 |
BBa_K3429007 | Terminator | degQ terminator from B. subtilis | 36 |
BBa_K3429008 | Promotor | promotor P-CymRC | 90 |
BBa_K3429009 | Promotor | promotor P-SalTCC | 116 |
BBa_K3429011 | Coding Sequence | Laccase CotA | 1542 |
BBa_K3429012 | Coding Sequence | Blue copper oxidase CueO | 1587 |
Composite Parts
For in vitro characterization
For purification and in vitro characterization of our enzymes we designed the following Composite Parts : cotA, cueO, and ereB. All of these Composite Parts contain a strep-tag for purification and are optimized for E. coli.
For Immobilization on our biofilm
The following parts were designed for immobilization of our enzymes on the TasA matrix protein of our B. subtilis biofilm. In these composite parts the enzymes are genetically fused to TasA. We modeled the structure of the TasA-EreB fusion protein via MD simulation to see whether the enzyme remains stable (BBa_K3429013).
For our kill switch
Hier ne Abbildung?Registry Parts
Kleiner Text zu
Part(BBa_) | Type | Description | Length in bp |
---|---|---|---|