Difference between revisions of "Team:TU Darmstadt/Parts"
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| − | <div class="containertext">For our project design we <b>took advantage of the iGEM registry</b> and took several parts from it. The parts are listed in the table below. For the well characterized part <a href="http://parts.igem.org/Part:BBa_K1159000" target="_blank">BBa_K1159000</a> from iGEM TU Munich 2013 we <b>generated the missing <a href="https://2020.igem.org/Team:TU_Darmstadt/Model/Enzyme_Modeling#EreB_CM" target="_blank">modelling data</a></b>. For <a href="http://parts.igem.org/Part:BBa_K1680007" target="_blank"> BBa_K1680007</a> we provide <b>further literature information</b>. | + | <div class="containertext">For our project design we <b>took advantage of the iGEM registry</b> and took several parts from it. The parts are listed in the table below. For the well characterized part <a href="http://parts.igem.org/Part:BBa_K1159000" target="_blank">BBa_K1159000</a> from iGEM TU Munich 2013 we <b>generated the missing <a href="https://2020.igem.org/Team:TU_Darmstadt/Model/Enzyme_Modeling#EreB_CM" target="_blank">modelling data</a></b>. For <a href="http://parts.igem.org/Part:BBa_K1680007" target="_blank"> BBa_K1680007</a> we provide <b>further literature information</b>. Parts we submit for the medal criteria <b>contribution</b> are also marked with this symbol: ♥. |
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| − | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1680007" target="_blank">BBa_K1680007</a></th> | + | <td scope="row">♥ <a href="http://parts.igem.org/Part:BBa_K1680007" target="_blank">BBa_K1680007</a></th> |
<td>Coding Sequence</td> | <td>Coding Sequence</td> | ||
<td>Cre recombinase</td> | <td>Cre recombinase</td> | ||
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| − | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1159000" target="_blank">BBa_K1159000</a></th> | + | <td scope="row">♥ <a href="http://parts.igem.org/Part:BBa_K1159000" target="_blank">BBa_K1159000</a></th> |
<td>Coding Sequence</td> | <td>Coding Sequence</td> | ||
<td>Erythromycin Esterase Type II (EreB) in RFC[25]</td> | <td>Erythromycin Esterase Type II (EreB) in RFC[25]</td> | ||
Revision as of 13:38, 22 October 2020
Due to COVID-19 we were not able to test any of our designed parts in the lab. Nevertheless, we registered our basic parts, so future teams are easily able to characterize them. For our Composite Parts we only uploaded one for which we can provide modeling data. We refrained from uploading the others to the registry as there exists no primary literature on them, but we still provide schematic images on them. We did not submit any parts for the special prizes Best Basic Part and Best Composite Part.
Basic Parts
All basic parts we implemented into the design of our are listed in the table below.
| Part(BBa_) | Type | Description | Length in bp |
|---|---|---|---|
| BBa_K3429000 | Promoter | Pgrac promoter | 170 |
| BBa_K3429001 | Coding Sequence | TasA matrix protein | 783 |
| BBa_K3429002 | Coding Sequence | Superfolder Green Fluorescent Protein (sfGFP) | 712 |
| BBa_K3429003 | Coding Sequence | Protein Linker for fusion proteins: ARGGGGSGGGGSGS | 42 |
| BBa_K3429004 | Terminator | trpA terminator | 24 |
| BBa_K3429005 | Coding Sequence | Ribosomal Protein S2 (rpsB) | 721 |
| BBa_K3429006 | Promoter | PdegQ promoter | 192 |
| BBa_K3429007 | Terminator | degQ terminator | 36 |
| BBa_K3429011 | Coding Sequence | Laccase CotA | 1542 |
| BBa_K3429012 | Coding Sequence | Blue copper oxidase CueO | 1587 |
Composite Parts
For in vitro Characterization
For purification and in vitro characterization of our enzymes we designed the following Composite Parts : cotA, cueO, and ereB. All of these Composite Parts contain a strep-tag for purification and are optimized for E. coli.
For Immobilization on our Biofilm
The following parts were designed for immobilization of our enzymes on the TasA matrix protein of our B. subtilis biofilm. In these composite parts the enzymes are genetically fused to TasA. We modeled the structure of the TasA-EreB fusion protein via MD simulation to see whether the enzyme remains stable (BBa_K3429013).
For our Kill Switch
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Registry Parts
For our project design we took advantage of the iGEM registry and took several parts from it. The parts are listed in the table below. For the well characterized part BBa_K1159000 from iGEM TU Munich 2013 we generated the missing modelling data. For BBa_K1680007 we provide further literature information. Parts we submit for the medal criteria contribution are also marked with this symbol: ♥.
| Part(BBa_) | Type | Description | Length in bp | Part created by |
|---|---|---|---|---|
| BBa_K733002 | Promoter | PXylA promoter | 1387 | iGEM HKUST Hong Kong 2012 |
| BBa_K823003 | Promoter | Pveg promoter | 237 | iGEM LMU Munich 2012 |
| ♥ BBa_K1680007 | Coding Sequence | Cre recombinase | 1029 | iGEM Tuebingen 2015 |
| BBa_I718016 | Recombination Site | lox66 site | 34 | iGEM Paris 2007 |
| BBa_I718017 | Recombination Site | lox71 site | 34 | iGEM Paris 2007 |
| ♥ BBa_K1159000 | Coding Sequence | Erythromycin Esterase Type II (EreB) in RFC[25] | 1254 | iGEM TU Munich 2013 |