Difference between revisions of "Team:TU Darmstadt/Partnership/Summary And Methods"
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<td scope="row">Used Enzymes</th> | <td scope="row">Used Enzymes</th> | ||
| − | <td>laccases: CotA of <i>Bacillus subtilis</i> and CueO of <i>Escherichia coli</i> | + | <td>laccases: CotA of <i>Bacillus subtilis</i> and CueO of <i>Escherichia coli</i> |
| − | <br>esterase: EreB of <i>Escherichia coli</i></br></td> | + | <br>esterase: EreB of <i>Escherichia coli</i></br></td> |
| − | <td>Laccase of <i>Botrytis aclada</i> and marine unknown</td> | + | <td>Laccase of <i>Botrytis aclada</i> and marine unknown</td> |
| − | <td>Laccases of <i>Trametes versicolor</i> and <i>S. cyaneus</i> </td> | + | <td>Laccases of <i>Trametes versicolor</i> and <i>S. cyaneus</i> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td scope="row">Model Organism</th> | <td scope="row">Model Organism</th> | ||
| − | <td><i> | + | <td><i>Bacillus subtilis</i> and <i>Escherichia coli</i></td> |
| − | <td>green algae <i>Chlamydomonas reinhardtii</i> and <i>Escherichia coli</i> as comparison</td> | + | <td>green algae <i>Chlamydomonas reinhardtii</i> and <i>Escherichia coli</i> as comparison</td> |
| − | <td><i>Pichia pastoris</i> and <i> | + | <td><i>Pichia pastoris</i> and <i>Escherichia coli </i></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td scope="row">Special Technique</th> | <td scope="row">Special Technique</th> | ||
| − | <td>immobilization of the enzymes in the matrix of the <i>Bacillus subtilis</i> biofilm</td> | + | <td>immobilization of the enzymes in the matrix of the <i>Bacillus subtilis</i> biofilm</td> |
<td>laccase secretion and filtration before access to wastewater</td> | <td>laccase secretion and filtration before access to wastewater</td> | ||
<td>Immobilization of the enzymes in a silica foam </td> | <td>Immobilization of the enzymes in a silica foam </td> | ||
| Line 134: | Line 134: | ||
<br>Genomic Integration: Homologous Recombination</br> | <br>Genomic Integration: Homologous Recombination</br> | ||
</td> | </td> | ||
| − | <td><i>Chlamydomonas reinhardtii</i>: MoClo | + | <td><i>Chlamydomonas reinhardtii</i>: MoClo |
| − | <br><i>Escherichia coli</i>: ordered/ synthesized plasmid</br></td> | + | <br><i>Escherichia coli</i>: ordered/ synthesized plasmid</br></td> |
<td>1) Gibson Assembly | <td>1) Gibson Assembly | ||
<br>2) Restriction digestion</br> | <br>2) Restriction digestion</br> | ||
Revision as of 13:52, 4 October 2020
General Workflow
iGEM TU Darmstadt
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iGEM TU Kaiserslautern
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iGEM Stuttgart
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Project Comparison Table
| Issue | Darmstadt | Kaiserslautern | Stuttgart |
|---|---|---|---|
| Used Enzymes | laccases: CotA of Bacillus subtilis and CueO of Escherichia coli
esterase: EreB of Escherichia coli |
Laccase of Botrytis aclada and marine unknown | Laccases of Trametes versicolor and S. cyaneus |
| Model Organism | Bacillus subtilis and Escherichia coli | green algae Chlamydomonas reinhardtii and Escherichia coli as comparison | Pichia pastoris and Escherichia coli |
| Special Technique | immobilization of the enzymes in the matrix of the Bacillus subtilis biofilm | laccase secretion and filtration before access to wastewater | Immobilization of the enzymes in a silica foam |
| Tested Substrates | Laccases:
ABTS, Diclofenac
EreB: Erythromycin, Azithromycin |
ABTS | ABTS, Diclofenac, Carbamazepine |
| Genetic Engineering | Construct Assembly: Gibson Assembly
Genomic Integration: Homologous Recombination |
Chlamydomonas reinhardtii: MoClo
Escherichia coli: ordered/ synthesized plasmid |
1) Gibson Assembly
2) Restriction digestion |
| Fun Facts | only team of RLP |
Wastewater Treatment Plant Implementation