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| span.bgMenu, i.bgMenu, a.bgMenu { | | span.bgMenu, i.bgMenu, a.bgMenu { |
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| <!--This is the first row of the poster, it contains the title section ---> | | <!--This is the first row of the poster, it contains the title section ---> |
| <div class="row"> | | <div class="row"> |
− | <div class="section half first_text" id="abstract"> | + | <section><div class="section half first_text" id="abstract" tabindex="2"> |
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| <!--Here is the image used for the full width section, the size is: 1200 x 200 pixels --> | | <!--Here is the image used for the full width section, the size is: 1200 x 200 pixels --> |
| <img src="https://static.igem.org/mediawiki/2020/1/13/T--Fudan--Poster_bone_appetit3.png" alt="abstract" /> | | <img src="https://static.igem.org/mediawiki/2020/1/13/T--Fudan--Poster_bone_appetit3.png" alt="abstract" /> |
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| <!--Info: Place the title and text for this section, it will appear when the user clicks on it | | <!--Info: Place the title and text for this section, it will appear when the user clicks on it |
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| <div class="title"> Bone appetite </div> | | <div class="title"> Bone appetite </div> |
− | <!--Write the text explaining this section -->
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| <div class="text"> | | <div class="text"> |
| <ul> | | <ul> |
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| <p>We introduced the quorum sensing system to colonize bacteria in the intestine. Once the bacteria have successfully colonized the intestinal tract, the luxPR promoter will be activated and a series of short peptides that can bind to calcium and promote calcium absorption of intestinal epithelial cells will be expressed. A kill switch is added accordingly for safety.</p> | | <p>We introduced the quorum sensing system to colonize bacteria in the intestine. Once the bacteria have successfully colonized the intestinal tract, the luxPR promoter will be activated and a series of short peptides that can bind to calcium and promote calcium absorption of intestinal epithelial cells will be expressed. A kill switch is added accordingly for safety.</p> |
| <p>Our project provides a novel delivery method to provide calcium supplements sustainably. And we are actively seeking commercialization possibilities to expand our project impact, to reach more population, for the elderly's good health and well-being.</p> | | <p>Our project provides a novel delivery method to provide calcium supplements sustainably. And we are actively seeking commercialization possibilities to expand our project impact, to reach more population, for the elderly's good health and well-being.</p> |
− | </div> | + | </div> |
| </div> | | </div> |
− | </div> | + | </div></section> |
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− | <div class="section half" id="whatwehavedone"><!-- it will be the default section loaded .first_text --> | + | <section><div class="section half" id="whatwehavedone" tabindex="3"> |
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| <img src="https://static.igem.org/mediawiki/2020/d/d9/T--Fudan--Poster_todays_special3.png" alt="what we have done" /> | | <img src="https://static.igem.org/mediawiki/2020/d/d9/T--Fudan--Poster_todays_special3.png" alt="what we have done" /> |
| | | |
| <div class="info"> | | <div class="info"> |
| <div class="title"> 🎉🎉 What we have achieved 🎉🎉 </div> | | <div class="title"> 🎉🎉 What we have achieved 🎉🎉 </div> |
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| <div class="text"> | | <div class="text"> |
| <h4>Where we came from</h4> | | <h4>Where we came from</h4> |
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| More than half of our respondents aged between 40 and 60 replied that they know more than 3 seniors with fractures from falls. We realized that health issues surrounding bone health are no longer figures on paper but tangible challenges in many families. <span class="bgMenu">Calcium deficiency is clearly an urgent issue that requires immediate change.</span> However, the current calcium supplementation approaches are inefficient and not user-friendly, particularly towards the elderly. In early 2020, we initiated <i>Bone Appetit</i>, a project that strives to build efficient, user-friendly and sustainable calcium supplementation for the elderly.</p> | | More than half of our respondents aged between 40 and 60 replied that they know more than 3 seniors with fractures from falls. We realized that health issues surrounding bone health are no longer figures on paper but tangible challenges in many families. <span class="bgMenu">Calcium deficiency is clearly an urgent issue that requires immediate change.</span> However, the current calcium supplementation approaches are inefficient and not user-friendly, particularly towards the elderly. In early 2020, we initiated <i>Bone Appetit</i>, a project that strives to build efficient, user-friendly and sustainable calcium supplementation for the elderly.</p> |
| <h4>Where we are now</h4> | | <h4>Where we are now</h4> |
− | <p>In the past 10 months, we combined our work in lab, production, entrepreneurship, education and safety. We developed a thorough proof of our concept involving modeling a kill switch, designing and testing secretion peptides guided calcium absorption peptide (<span class="span_click" onclick=" | + | <p>In the past 10 months, we combined our work in lab, production, entrepreneurship, education and safety. We developed a thorough proof of our concept involving modeling a kill switch, designing and testing secretion peptides guided calcium absorption peptide (<span class="span_click" onclick="window.location='#demo_CaAP'">CaAP</span>), designing, modeling and testing a <span class="span_click" onclick="window.location='#demo_mcbABCD'">MccB17</span> based quorum sensing system, and developing a robust measurement scheme (<span class="span_click" onclick="window.location='#demo_CaAsst'">CaAsst</span>).</p> |
− | $('#section_title').empty();
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− | var pp_title = $('#demo_CaAP > .info > .title').html();
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− | $('#section_title').append(pp_title);
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− | $('#section_text').append(pp_text);
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− | ">CaAP</span>), designing, modeling and testing a <span class="span_click" onclick=" | + | |
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− | var pp_title = $('#demo_mcbABCD > .info > .title').html();
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− | ">MccB17</span> based quorum sensing system, and developing a robust measurement scheme (<span class="span_click" onclick=" | + | |
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− | ">CaAsst</span>).</p> | + | |
| <p>Our overall engineering success could not be achieved without the help of modeling and learning from the experienced. We modeled the initiation and steady states of a quorum sensing system.<br/> | | <p>Our overall engineering success could not be achieved without the help of modeling and learning from the experienced. We modeled the initiation and steady states of a quorum sensing system.<br/> |
| <img src="https://static.igem.org/mediawiki/2020/f/fa/T--Fudan--Poster_model-AHL-reproduction.jpg" alt="AHL production simulation" /><br/> | | <img src="https://static.igem.org/mediawiki/2020/f/fa/T--Fudan--Poster_model-AHL-reproduction.jpg" alt="AHL production simulation" /><br/> |
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| <p>In order to deliver quality education to the elderly, we started educating the young and instruct them to educate their parents and/or grandparents. We formed <a href="/Team:Fudan/Partnership" target="_blank">partnerships</a> with other iGEM teams to better educate the young and those from the rural areas. We actively reached out to high school students, undergraduates and all age groups with accessible educational opportunities.</p> | | <p>In order to deliver quality education to the elderly, we started educating the young and instruct them to educate their parents and/or grandparents. We formed <a href="/Team:Fudan/Partnership" target="_blank">partnerships</a> with other iGEM teams to better educate the young and those from the rural areas. We actively reached out to high school students, undergraduates and all age groups with accessible educational opportunities.</p> |
| <h4>Our contributions to #iGEM</h4> | | <h4>Our contributions to #iGEM</h4> |
− | <p>To conclude, our team has contributed to the iGEM community in various aspects, from <a href="/Team:Fudan/Inclusion" target="_blank">inclusive</a> <a href="/Team:Fudan/Education" target="_blank">education</a> to contributing <a href="http://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2020&group=Fudan" target="_blank">new parts to the Registry</a>, from <a href="/Team:Fudan/Model" target="_blank">improved modeling</a> to <a href="/Team:Fudan/Measurement" target="_blank">a robust measurement</a> and <a href="/Team:Fudan/Entrepreneurship" target="_blank">a product development plan</a>. We hope that our project is not just limited to providing <span class="bgMenu">solid foundations for future research</span> to build upon, but also contributes to achieving <a class="bgMenu" href="https://www.un.org/sustainabledevelopment/sustainable-development-goals/" target="_blank">the sustainable development goals</a> outlined by the United Nations.</p> | + | <p>To conclude, our team has contributed to the iGEM community in various aspects, from <a href="/Team:Fudan/Inclusion" target="_blank">inclusive</a> <a href="/Team:Fudan/Education" target="_blank">education</a> to contributing <a href="/Team:Fudan/Parts" target="_blank">new parts</a> <a href="http://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2020&group=Fudan" target="_blank">to the Registry</a>, from <a href="/Team:Fudan/Model" target="_blank">improved modeling</a> to <a href="/Team:Fudan/Measurement" target="_blank">a robust measurement</a> and <a href="/Team:Fudan/Entrepreneurship" target="_blank">a product development plan</a>. We hope that our project is not just limited to providing <span class="bgMenu">solid foundations for future research</span> to build upon, but also contributes to achieving <a class="bgMenu" href="https://www.un.org/sustainabledevelopment/sustainable-development-goals/" target="_blank">the sustainable development goals</a> outlined by the United Nations.</p> |
| </div> | | </div> |
| </div> | | </div> |
− | </div> | + | </div></section> |
| </div> | | </div> |
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| <div class="row"> | | <div class="row"> |
− | <!-- This creates a third width section ---> | + | <section><div class="section third" id="sdg3" tabindex="4"> |
− | <div class="section third" id="sdg3">
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− | <!--Here is the image used for the third width section, the size is: 400 x 200 pixels -->
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| <img src="https://static.igem.org/mediawiki/2020/a/a2/T--Fudan--Poster_sdg3e.png" alt="sdg3" /> | | <img src="https://static.igem.org/mediawiki/2020/a/a2/T--Fudan--Poster_sdg3e.png" alt="sdg3" /> |
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| </div> | | </div> |
| </div> | | </div> |
− | </div> | + | </div></section> |
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− | <div class="section third" id="sdg4"> | + | <section><div class="section third" id="sdg4" tabindex="5"> |
| <img src="https://static.igem.org/mediawiki/2020/2/2f/T--Fudan--Poster_sdg4ee.png" alt="sdg4" /> | | <img src="https://static.igem.org/mediawiki/2020/2/2f/T--Fudan--Poster_sdg4ee.png" alt="sdg4" /> |
| | | |
| <div class="info"> | | <div class="info"> |
| <div class="title"> SDG 4: QUALITY EDUCATION </div> | | <div class="title"> SDG 4: QUALITY EDUCATION </div> |
| + | |
| <div class="text"> | | <div class="text"> |
| <p><i class="bgMenu">Ensure inclusive and equitable quality education and promote lifelong learning opportunities for all.</i></p> | | <p><i class="bgMenu">Ensure inclusive and equitable quality education and promote lifelong learning opportunities for all.</i></p> |
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| </div> | | </div> |
| </div> | | </div> |
− | </div> | + | </div></section> |
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− | <div class="section third" id="sdg9"> | + | <section><div class="section third" id="sdg9" tabindex="6"> |
| <img src="https://static.igem.org/mediawiki/2020/d/d8/T--Fudan--Poster_sdg9e.png" alt="sdg9" /> | | <img src="https://static.igem.org/mediawiki/2020/d/d8/T--Fudan--Poster_sdg9e.png" alt="sdg9" /> |
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| <div class="info"> | | <div class="info"> |
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− | <!--Write the title of the section -->
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| <div class="title"> SDG 9: INDUSTRY, INNOVATION AND INFRASTRUCTURE </div> | | <div class="title"> SDG 9: INDUSTRY, INNOVATION AND INFRASTRUCTURE </div> |
− | <!--Write the text explaining this section -->
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| <div class="text"> | | <div class="text"> |
| <p><i class="bgMenu">Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation.</i></p> | | <p><i class="bgMenu">Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation.</i></p> |
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| <p>Our project aims at converting engineered probiotic into a sustainable calcium supplement ready for the market. Our product targets seniors suffering calcium deficiency and strives to lower the risks of osteoporosis amongst seniors. Our product is highly efficient and does not require frequent intake.</p> | | <p>Our project aims at converting engineered probiotic into a sustainable calcium supplement ready for the market. Our product targets seniors suffering calcium deficiency and strives to lower the risks of osteoporosis amongst seniors. Our product is highly efficient and does not require frequent intake.</p> |
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− | <p>Our product is a genetically engineered probiotic that can colonize the intestine and secrete <span class="span_click" onclick=" | + | <p>Our product is a genetically engineered probiotic that can colonize the intestine and secrete <span class="span_click" onclick="window.location='#demo_CaAP'">a short peptide - CaAP</span>, which increases the amount of soluble calcium by binding to calcium ions and increasing the absorption of calcium by intestinal epithelium. Compared with other calcium supplement products such as calcium pills, our product has its unique advantages.<br/> |
− | $('#section_title').empty();
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− | ">a short peptide - CaAP</span>, which increases the amount of soluble calcium by binding to calcium ions and increasing the absorption of calcium by intestinal epithelium. Compared with other calcium supplement products such as calcium pills, our product has its unique advantages.<br/> | + | |
| <img src="https://static.igem.org/mediawiki/2020/b/bf/T--Fudan--Poster_filler.jpg" alt="to be replaced" /></p> | | <img src="https://static.igem.org/mediawiki/2020/b/bf/T--Fudan--Poster_filler.jpg" alt="to be replaced" /></p> |
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| </div> | | </div> |
| </div> | | </div> |
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| <div class="row"> | | <div class="row"> |
− | <!-- This creates a third width section with the id 'section_1' ---> | + | <section><div class="section third" id="demo_CaAP" tabindex="7"> |
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| <img src="https://static.igem.org/mediawiki/2020/8/89/T--Fudan--Poster_caapeend.png" alt="demo CaAP" /> | | <img src="https://static.igem.org/mediawiki/2020/8/89/T--Fudan--Poster_caapeend.png" alt="demo CaAP" /> |
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| <div class="info"> | | <div class="info"> |
| <div class="title"> We invented CaAP </div> | | <div class="title"> We invented CaAP </div> |
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| <div class="text"> | | <div class="text"> |
| <p>CaAP stands for <u>Ca</u>lcium <u>A</u>bsorption <u>P</u>eptide.<br/> | | <p>CaAP stands for <u>Ca</u>lcium <u>A</u>bsorption <u>P</u>eptide.<br/> |
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| <p>Driven by a quorum sensing promoter, <i>E. coli</i> only express signal peptide linked CaAP after their colonization. We demonstrated <i>in vitro</i> released CaAP by SDS-PAGE, in trichloroacetic acid precipitated bacterial culture media samples.<br/> | | <p>Driven by a quorum sensing promoter, <i>E. coli</i> only express signal peptide linked CaAP after their colonization. We demonstrated <i>in vitro</i> released CaAP by SDS-PAGE, in trichloroacetic acid precipitated bacterial culture media samples.<br/> |
| <img src="https://static.igem.org/mediawiki/2020/b/bf/T--Fudan--Poster_filler.jpg" alt="to be replaced" /></p> | | <img src="https://static.igem.org/mediawiki/2020/b/bf/T--Fudan--Poster_filler.jpg" alt="to be replaced" /></p> |
− | </div> | + | </div> |
| </div> | | </div> |
− | </div> | + | </div></section> |
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− | <!-- This creates a third width section with the id 'section_2' ---> | + | <section><div class="section third" id="demo_mcbABCD" tabindex="8"> |
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| <img src="https://static.igem.org/mediawiki/2020/2/2c/T--Fudan--Poster_mcbee.png" alt="demo mcbABCD" /> | | <img src="https://static.igem.org/mediawiki/2020/2/2c/T--Fudan--Poster_mcbee.png" alt="demo mcbABCD" /> |
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| <div class="info"> | | <div class="info"> |
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| <div class="title"> We improved MccB17 expression </div> | | <div class="title"> We improved MccB17 expression </div> |
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| <div class="text"> | | <div class="text"> |
− | <p>Based on previous work, we use AHL+LuxR, TetR and the antimicrobial peptide microcin B17 (MccB17) to achieve an on/off switch-like controlling of bacterial colonization and <span class="span_click" onclick=" | + | <p>Based on previous work, we use AHL+LuxR, TetR and the antimicrobial peptide microcin B17 (MccB17) to achieve an on/off switch-like controlling of bacterial colonization and <span class="span_click" onclick="window.location='#demo_CaAP'">CaAP</span> expression.</p> |
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− | ">CaAP</span> expression.</p> | + | |
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| <p>McbA,B,C,D jointly complete the secretion of MccB17. Among them, peptide product from <i>mcbA</i> is the precursor of the antimicrobial peptide, and McbB,C,D is responsible for the post-translational modification of McbA, to produce MccB17. McbE,F assemble the channel to export MccB17. McbG provides the immunity against MccB17. If the engineered bacteria express only McbA,B,C,D, but neither McbE,F nor McbG, intracellular accumulated MccB17 will kill itself. When the surrounding bacteria absorb MccB17 but not McbG, they would die.</p> | | <p>McbA,B,C,D jointly complete the secretion of MccB17. Among them, peptide product from <i>mcbA</i> is the precursor of the antimicrobial peptide, and McbB,C,D is responsible for the post-translational modification of McbA, to produce MccB17. McbE,F assemble the channel to export MccB17. McbG provides the immunity against MccB17. If the engineered bacteria express only McbA,B,C,D, but neither McbE,F nor McbG, intracellular accumulated MccB17 will kill itself. When the surrounding bacteria absorb MccB17 but not McbG, they would die.</p> |
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| <img src="https://static.igem.org/mediawiki/2020/b/bf/T--Fudan--Poster_filler.jpg" alt="to be replaced by modeling" /></p> | | <img src="https://static.igem.org/mediawiki/2020/b/bf/T--Fudan--Poster_filler.jpg" alt="to be replaced by modeling" /></p> |
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− | <p>At this time, expressed TetR transcription factor will turn off PtetR. As a result, <i>mcbEFG</i> stops expressing, and <span class="span_click" onclick=" | + | <p>At this time, expressed TetR transcription factor will turn off PtetR. As a result, <i>mcbEFG</i> stops expressing, and <span class="span_click" onclick="window.location='#demo_CaAP'">CaAP</span> begins to be produced.</p> |
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− | ">CaAP</span> begins to be produced.</p> | + | |
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| <p>Improved from last year, we: <span class="bgMenu">(1)</span> use low-copy plasmid backbone pFAB to reduce the expression burden on engineered bacteria; <span class="bgMenu">(2)</span> low expression promoters to drive <i>mcbABCD</i>; <span class="bgMenu">(3)</span> PtetR drives <i>mcbEFG</i> from the reverse strand, to avoid translational readthrough; <span class="bgMenu">(4)</span> validate individual McbA/B/C/D/E/F/G expression in an IPTG induction setting.</p> | | <p>Improved from last year, we: <span class="bgMenu">(1)</span> use low-copy plasmid backbone pFAB to reduce the expression burden on engineered bacteria; <span class="bgMenu">(2)</span> low expression promoters to drive <i>mcbABCD</i>; <span class="bgMenu">(3)</span> PtetR drives <i>mcbEFG</i> from the reverse strand, to avoid translational readthrough; <span class="bgMenu">(4)</span> validate individual McbA/B/C/D/E/F/G expression in an IPTG induction setting.</p> |
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| <br/><img src="https://static.igem.org/mediawiki/2020/f/fb/T--Fudan--Poster_flowchart-IPTG.png" alt="flow chart of MccB17 IPTG experiments" /> | | <br/><img src="https://static.igem.org/mediawiki/2020/f/fb/T--Fudan--Poster_flowchart-IPTG.png" alt="flow chart of MccB17 IPTG experiments" /> |
| </p> | | </p> |
− | </div> | + | </div> |
| </div> | | </div> |
− | </div> | + | </div></section> |
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− | <!-- This creates a third width section with the id 'section_3' ---> | + | <section><div class="section third" id="demo_CaAsst" tabindex="9"> |
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| <img src="https://static.igem.org/mediawiki/2020/d/db/T--Fudan--Poster_measee.png" alt="demo CaAsst" /> | | <img src="https://static.igem.org/mediawiki/2020/d/db/T--Fudan--Poster_measee.png" alt="demo CaAsst" /> |
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| <div class="info"> | | <div class="info"> |
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| <div class="title"> We developed CaAsst for measuring Ca<sup>2+</sup> </div> | | <div class="title"> We developed CaAsst for measuring Ca<sup>2+</sup> </div> |
− | <!--Write the text explaining this section -->
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| <div class="text"> | | <div class="text"> |
| <p>Both calcium deficiency or high blood calcium leads to many diseases and severely reduces the quality of life. Therefore, it is important to monitor blood calcium levels when taking calcium supplements.</p> | | <p>Both calcium deficiency or high blood calcium leads to many diseases and severely reduces the quality of life. Therefore, it is important to monitor blood calcium levels when taking calcium supplements.</p> |
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| <img src="https://static.igem.org/mediawiki/2020/7/75/T--Fudan--Poster_Ca2withInterfere.png" alt="prism graph of Ca2+ with interference" /></p> | | <img src="https://static.igem.org/mediawiki/2020/7/75/T--Fudan--Poster_Ca2withInterfere.png" alt="prism graph of Ca2+ with interference" /></p> |
| <p><span class="bgMenu">Further development</span> may focus on improving the stability of application solutions, making glucometer compatible test strips, and low-cost-and-portable measuring hardware.</p> | | <p><span class="bgMenu">Further development</span> may focus on improving the stability of application solutions, making glucometer compatible test strips, and low-cost-and-portable measuring hardware.</p> |
− | </div> | + | </div> |
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− | </div> | + | </div></section> |
| </div> | | </div> |
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− | <div class="section half" id="parts"> | + | <section><div class="section half" id="parts" tabindex="10"> |
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| <img src="https://static.igem.org/mediawiki/2020/f/fd/T--Fudan--Poster_how1.png" alt="parts" /> | | <img src="https://static.igem.org/mediawiki/2020/f/fd/T--Fudan--Poster_how1.png" alt="parts" /> |
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| <div class="info"> | | <div class="info"> |
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| <div class="title"> How to use our parts </div> | | <div class="title"> How to use our parts </div> |
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| <div class="text"> | | <div class="text"> |
− | <p>Our part collection offers a complete array of parts for future researchers to develop other probiotic secreted proteins/peptides. This year, we have <span class="span_click" onclick=" | + | <p>Our part collection offers a complete array of parts for future researchers to develop other probiotic secreted proteins/peptides. This year, we have <span class="span_click" onclick="window.location='#demo_mcbABCD'">MccB17</span> and <span class="span_click" onclick="window.location='#demo_CaAP'">CaAP</span> secreted and experimentally verified.</p> |
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− | ">MccB17</span> and <span class="span_click" onclick=" | + | |
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− | ">CaAP</span> secreted and experimentally verified.</p> | + | |
| <p>We contributed 3 probiotic signal peptides (<a href="http://parts.igem.org/Part:BBa_K3606042" target="_blank">NSP4</a>, <a href="http://parts.igem.org/Part:BBa_K3606043" target="_blank">OmpA</a>, <a href="http://parts.igem.org/Part:BBa_K3606030" target="_blank">DsbA</a>), 14 low-expression promoters (BBa_K3606008 ~ BBa_K3606023) in a low-copy plasmid backbone, PtetR promoter on the reverse strand (BBa_K3606030), mcbEFG on the reverse strand (BBa_K3606821), luxPR promoter driven various proteins, PlacIq_lacI_Ptac promoter driven various proteins, <a href="http://parts.igem.org/Part:BBa_K3606007" target="_blank">PtetO3</a> (BBa_K3606007, improved from <a href="http://parts.igem.org/Part:BBa_R0040" target="_blank">PtetR</a>), <i>etc</i>.</p> | | <p>We contributed 3 probiotic signal peptides (<a href="http://parts.igem.org/Part:BBa_K3606042" target="_blank">NSP4</a>, <a href="http://parts.igem.org/Part:BBa_K3606043" target="_blank">OmpA</a>, <a href="http://parts.igem.org/Part:BBa_K3606030" target="_blank">DsbA</a>), 14 low-expression promoters (BBa_K3606008 ~ BBa_K3606023) in a low-copy plasmid backbone, PtetR promoter on the reverse strand (BBa_K3606030), mcbEFG on the reverse strand (BBa_K3606821), luxPR promoter driven various proteins, PlacIq_lacI_Ptac promoter driven various proteins, <a href="http://parts.igem.org/Part:BBa_K3606007" target="_blank">PtetO3</a> (BBa_K3606007, improved from <a href="http://parts.igem.org/Part:BBa_R0040" target="_blank">PtetR</a>), <i>etc</i>.</p> |
| <p>We not only provide sequences and references for these parts, but also experimental results and procedures. Most of the parts were assembled together using high-fidelity PCR and ClonExpress ligation reactions. All plasmids were sequencing verified and available upon email request <span class="bgMenu">igem@fudan.edu.cn</span>.</p> | | <p>We not only provide sequences and references for these parts, but also experimental results and procedures. Most of the parts were assembled together using high-fidelity PCR and ClonExpress ligation reactions. All plasmids were sequencing verified and available upon email request <span class="bgMenu">igem@fudan.edu.cn</span>.</p> |
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| <img src="https://static.igem.org/mediawiki/2020/9/9d/T--Fudan--Poster_ack1.png" alt="references" /> | | <img src="https://static.igem.org/mediawiki/2020/9/9d/T--Fudan--Poster_ack1.png" alt="references" /> |
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| <div class="title"> Acknowledgements </div> | | <div class="title"> Acknowledgements </div> |
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| <div class="text"> | | <div class="text"> |
− | <p>Our project wouldn’t be complete without the help of many dedicated people. <span class="bgMenu">Bone appetite</span>.</p> | + | <p>Our project wouldn’t be complete without the help of many dedicated people.</p> |
| <p>We give special thanks to the institutions, organizations, professors, experts, key stakeholders who have provided tremendous help to us along the way. Our project wouldn’t be complete without their dedication. </p> | | <p>We give special thanks to the institutions, organizations, professors, experts, key stakeholders who have provided tremendous help to us along the way. Our project wouldn’t be complete without their dedication. </p> |
| <p>We were also fortunate enough to have advisors, experts and senior undergraduate students who offered vital advice and contribution in the making of this project.</p> | | <p>We were also fortunate enough to have advisors, experts and senior undergraduate students who offered vital advice and contribution in the making of this project.</p> |
− | <p>We would also like the acknowledge our collaboration with other teams from the iGEM community, including 19Fudan, 19BNU-China, TJUSLS-China, SCU-China. They have helped us with perfecting education work and guided us through conducting experiments.</p> | + | <p>We would also like to acknowledge our collaboration with other teams from the iGEM community, including 19Fudan, 19BNU-China, TJUSLS-China, and SCU-China. They have helped us with perfecting education work and guided us through conducting experiments.</p> |
| <p>References: 只需要核心文献</p> | | <p>References: 只需要核心文献</p> |
| <ul> | | <ul> |
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