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− | We made different deletions of major TAG lipases TGL3, TGL4 and TGL5 and analyzed the effect of these modifications on the lipid levels. We found that deletion of TAG lipases results in a considerable increase in lipid levels, with the triple deletion having the greatest effect (Fig 1). | + | We made different deletions of major TAG lipases TGL3, TGL4 and TGL5 and analyzed the effect of these modifications on the lipid levels. We found that deletion of TAG lipases results in a considerable increase in lipid levels, with the triple deletion having the greatest effect (Fig 1). <br><br> |
− | <img src="https://static.igem.org/mediawiki/2020/b/bb/T--Estonia_TUIT--Poster_Results-Fig2.png"> | + | <img src="https://static.igem.org/mediawiki/2020/b/bb/T--Estonia_TUIT--Poster_Results-Fig2.png"> <br><br> |
<b>Figure 1.</b> Mean Nile red fluorescence intensities in single cells from the indicated strains. The bars show median and its 95% confidence intervals. ****, **, and ns note p-values <0.0001, <0.01, and >0.05, respectively, of pairwise comparisons with wild-type using Mann-Whitney U test. | <b>Figure 1.</b> Mean Nile red fluorescence intensities in single cells from the indicated strains. The bars show median and its 95% confidence intervals. ****, **, and ns note p-values <0.0001, <0.01, and >0.05, respectively, of pairwise comparisons with wild-type using Mann-Whitney U test. |
Revision as of 04:24, 11 November 2020