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− | First, we used an agent-based stochastic approach to model the effects of PAH1 and DGA1 overexpression combined with the reduction of TAG lipase activity. The ‘agents’ of the model are enzymatic reactions, for which the parameters can be changed. Overexpression of PAH1 and DGA1 led to an increased activity of PAP and DGAT enzymes that are responsible for the final steps in TAG synthesis. Reduced TAG lipase activity prevented the release of lipid molecules from lipid droplets. | + | First, we used an agent-based stochastic approach to model the effects of PAH1 and DGA1 overexpression combined with the reduction of TAG lipase activity. The ‘agents’ of the model are enzymatic reactions, for which the parameters can be changed. Overexpression of PAH1 and DGA1 led to an increased activity of PAP and DGAT enzymes that are responsible for the final steps in TAG synthesis. Reduced TAG lipase activity prevented the release of lipid molecules from lipid droplets.<br><br> |
<img src="https://static.igem.org/mediawiki/2020/b/b2/T--EStonia_TUIT--Poster_Model5.png"> | <img src="https://static.igem.org/mediawiki/2020/b/b2/T--EStonia_TUIT--Poster_Model5.png"> | ||
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<b><br><br>Survey:<br></b> | <b><br><br>Survey:<br></b> | ||
− | We conducted a public survey to estimate the potential customers’ | + | We conducted a public survey to estimate the potential customers’<br> |
− | 1. demand for different lipid-containing products; | + | 1. demand for different lipid-containing products;<br> |
− | 2. opinion on genetically modified organisms used in production; | + | 2. opinion on genetically modified organisms used in production;<br> |
− | 3. opinion on the environmental footprint of the production. | + | 3. opinion on the environmental footprint of the production.<br> |
The survey showed an increasing awareness of the younger generation about GMO products and the significance of environmental impact and also confirmed their demand for our lipid products. | The survey showed an increasing awareness of the younger generation about GMO products and the significance of environmental impact and also confirmed their demand for our lipid products. | ||
Revision as of 04:38, 11 November 2020