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Perilipins promote lipid droplet assembly. To take use of this, we introduced a construct where perilipin Plin3 was expressed from a light-inducible promoter. We found that when grown under light, these cells accumulated substantially higher concentration of lipids (Fig 2), presumably because the induced Plin3 stabilizes TAGs by supporting lipid droplet formation. | Perilipins promote lipid droplet assembly. To take use of this, we introduced a construct where perilipin Plin3 was expressed from a light-inducible promoter. We found that when grown under light, these cells accumulated substantially higher concentration of lipids (Fig 2), presumably because the induced Plin3 stabilizes TAGs by supporting lipid droplet formation. | ||
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− | <img src="https://static.igem.org/mediawiki/2020/ | + | <img src="https://static.igem.org/mediawiki/2020/f/f1/T--Estonia_TUIT--Poster_Results-Fig3.png"> |
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<b>Figure 2.</b> Microscopy images of exemplary cells showing the accumulation of lipids in LDs. (B) Plot showing the average fluorescence intensities of Nile red staining. The bars show median values and its 95% confidence intervals. **** indicates p-value <0.0001 in pairwise comparison by Mann-Whitney U test. | <b>Figure 2.</b> Microscopy images of exemplary cells showing the accumulation of lipids in LDs. (B) Plot showing the average fluorescence intensities of Nile red staining. The bars show median values and its 95% confidence intervals. **** indicates p-value <0.0001 in pairwise comparison by Mann-Whitney U test. | ||
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We used nanoparticles to give our production strain a new quality – the ability to use light as an energy source to drive lipid synthesis (Fig 3). For that, we developed a bioinorganic hybrid platform where a disruption in the oxidative portion of the pentose phosphate pathway by ZWF1 deletion, which renders the cell unable to regenerate NADPH, is complemented by electron donation from an illuminated semiconductor. The experiments show that these biohybrids accumulate higher levels of lipids when grown under light, indicating that they are using light to support the lipid production. | We used nanoparticles to give our production strain a new quality – the ability to use light as an energy source to drive lipid synthesis (Fig 3). For that, we developed a bioinorganic hybrid platform where a disruption in the oxidative portion of the pentose phosphate pathway by ZWF1 deletion, which renders the cell unable to regenerate NADPH, is complemented by electron donation from an illuminated semiconductor. The experiments show that these biohybrids accumulate higher levels of lipids when grown under light, indicating that they are using light to support the lipid production. | ||
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− | <img src="https://static.igem.org/mediawiki/2020/ | + | <img src="https://static.igem.org/mediawiki/2020/9/90/T--Estonia_TUIT--Poster_Results-Fig4.png"> |
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<b>Figure 3.</b> (A) Microscopy images of exemplary cells showing the accumulation of lipids in LDs detected by Nile red staining. (B) Plot showing the average fluorescence intensities of Nile red staining. The bars show median values and its 95% confidence intervals. **** indicates p-value <0.0001 in pair-wise comparison by Mann-Whitney U test. | <b>Figure 3.</b> (A) Microscopy images of exemplary cells showing the accumulation of lipids in LDs detected by Nile red staining. (B) Plot showing the average fluorescence intensities of Nile red staining. The bars show median values and its 95% confidence intervals. **** indicates p-value <0.0001 in pair-wise comparison by Mann-Whitney U test. |
Latest revision as of 12:42, 17 December 2020